Lumisphere-induced phagocytosis and chemiluminescence of human polymorphonuclear leukocytes of normal adults and the patients with various respiratory diseases.

Kohashi, Osamu; Kohashi, Yukiko; Shibata, Maki; Uchida, Takahumi; S, Hosaka; Hashimoto, Shuichi; Mitsuyama, Takasi; Shigematsu, Nobuaki

doi:10.2492/jsir1981.8.41

Cited by 2 publications

(3 citation statements)

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“…This burst consists of the release of chemically reactive molecules such as superoxide anions, hydrogen peroxide, hypochlorous acid, and hydroxyl radicals. The generation of these reactive oxygen species can be quantified in the presence of chemiluminogenic probes such as luminol (12,21). The luminol-dependent CL response, therefore, is a useful method for examining the oxygen-dependent killing activity of PMNs triggered by bacteria (10).…”

Section: Discussionmentioning

confidence: 99%

Effect of a factor released by K562 malignant cells in culture on human neutrophil bactericidal activity

Amar¹,

Amit²,

Babin-Chevaye³

et al. 1991

Infect Immun

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We have previously demonstrated that K562 malignant cells in culture contain and release a low-molecularmass (8-kDa) factor that inhibits adherence-related functions of neutrophils but does not alter fMet-Leu-Pheor phorbol ester-induced oxidative burst (M. Amar, N. Amit, T. Pham Huu, S. Chollet-Martin, M. T. Labro, M. A. Gougerot-Pocidalo, and J. Hakim, J. Immunol. 144:4749-4756, 1990). In this study, we investigated the effects of this factor, referred to as inhibitory factor 1 (IF1), on the bactericidal activity of human polymorphonuclear cells (PMNs) on Staphylococcus aureus opsonized in various ways. S. aureus was used either nonopsonized or opsonized with heat-inactivated serum or normal serum containing complement factors.The bactericidal activity of PMNs preincubated with IFl-treated or control medium was examined by counting the surviving bacteria. The ability of IFl-treated PMNs to kill bacteria was diminished when they were opsonized with normal serum. When S. aureus was not opsonized or was opsonized with heat-inactivated seruin, the bactericidal activity of IFl-treated PMNs was similar to that of controls. Likewise, the phagocytosis of IFl-treated PMNs was diminished when S. aureus was opsonized with normal serum but was not altered when S. aureus was not opsonized or was opsonized with heat-inactivated serum. These results suggest that the decrease in killing might be due to defective ingestion. The chemiluminescence response of IFl-treated PMNs was inhibited when S. aureus was not opsonized or was opsonized with normal serum. No effect on chemiluminescence was observed when S. aureus was opsonized with heat-inactivated serum. These results suggest that IF1 interferes not only with S. aureus stimulation of PMNs via complement receptors but also with oxygen-dependent bactericidal activity.

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Section: Discussionmentioning

confidence: 99%

Effect of a factor released by K562 malignant cells in culture on human neutrophil bactericidal activity

Amar¹,

Amit²,

Babin-Chevaye³

et al. 1991

Infect Immun

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show abstract

“…brane oxidase, which in turn triggers the respiratory burst and produces chemically reactive molecules such as superoxide anion (02), hydrogen peroxide (H202), singlet oxygen (102), and hydroxy radical (OH .). The generation of these reactive oxygen species can be quantified as CL in the presence of chemiluminogenic probes such as luminol (1,14,40,41). The luminol-dependent CL response, therefore, is likely to prove a useful method for examining the intracellular killing by PMNs.…”

Section: Discussionmentioning

confidence: 99%

“…It is already known that PMNs emit chemiluminescence (CL) during the process of phagocytosis, which correlates well with the bactericidal activity of PMNs (1,14,40). The purpose of this study was to investigate the effects of an important pathogen (B. gingivalis) on the bactericidal activity of PMNs by measuring the bacterial phagocytic killing, luminol-dependent CL, and superoxide anion (02-) production.…”

mentioning

confidence: 99%

Suppression of bactericidal activity of human polymorphonuclear leukocytes by Bacteroides gingivalis

1990

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The direct effects of the culture supernatant of oral microorganisms on the bactericidal activity of human polymorphonuclear leukocytes (PMNs) were investigated. The bactericidal activity of PMNs, which were preincubated with the supernatant of Bacteroides gingivalis, Bacteroides intermedius, Bacteroides melaninogenicus or phosphate-buffered saline, was examined by counting the surviving bacteria. B. gingivalis-treated PMNs were found to have a diminished ability for killing bacteria in the presence or absence of serum. The chemiluminescence response of PMNs, which were preincubated with the culture supernatant of B. gingivalis, was strikingly reduced compared with that of PMNs preincubated with phosphate-buffered saline or other bacterial culture supernatants. The production of superoxide anion (02-) by PMNs stimulated with either formyl-methionyl-leucyl-phenylalanine or phorbol myristate acetate was reduced in both cases after the PMNs were preincubated with the culture supernatant of B. gingivalis. However, it was observed that there was more reduction in superoxide anion (02) production stimulated with formyl-methionyl-leucyl-phenyalanine compared with that stimulated with phorbol myristate acetate. These results suggest that B. gingivalis releases a factor which interferes with the bactericidal activity of PMNs by modulating the generation of reactive oxygen species. These suppressive effects on bactericidal activity may be important in the pathogenesis of this microorganism. Chronic inflammatory periodontal disease is initiated by bacterial infection. The role of specific gram-negative bacteria in the etiology and pathogenesis of human periodontal disease has been increasingly appreciated in recent years (20, 37). It is generally accepted that Bacteroides gingivalis is closely associated with adult periodontitis (7, 11, 21, 23). Furthermore, the interrelationship between a progression of inflammatory periodontal disease and polymorphonuclear leukocytes (PMNs), which act as one of the host defense systems against bacterial infection, has been widely investigated (2, 4, 9, 15, 27, 44). It has been reported that patients with a diminished function of PMNs often suffered from severe periodontal breakdown (5, 6, 39). Loesche et al. (16) recently reported that gingival crevicular PMNs collected from diseased sites had a diminished ability to form oxidized products after phorbol myristate acetate (PMA) stimulation. This result suggests that the functions of PMNs are affected by the bacteria found in subgingival plaque at the site of periodontal pockets. Van Dyke (43) reported that the soluble products of periodontopathic microorganisms modulated the receptors of the PMNs. It has been also reported that several factors from B. gingivalis affected the function of PMNs (12, 19, 22, 24). We recently reported (18) that the soluble factor from B. gingivalis suppressed the superoxide anion (02-) production of PMNs via the modulation of cell surface receptors and internal cellular events. Because of this result,

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Lumisphere-induced phagocytosis and chemiluminescence of human polymorphonuclear leukocytes of normal adults and the patients with various respiratory diseases.

Cited by 2 publications

References 0 publications

Effect of a factor released by K562 malignant cells in culture on human neutrophil bactericidal activity

Effect of a factor released by K562 malignant cells in culture on human neutrophil bactericidal activity

Suppression of bactericidal activity of human polymorphonuclear leukocytes by Bacteroides gingivalis

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