Lung Cancer and Its Early Detection Using Biomarker-Based Biosensors

Arya, Sunil K.; Bhansali, Shekhar

doi:10.1021/cr100420s

Cited by 247 publications

(128 citation statements)

References 103 publications

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“…These methods are invasive, time‐consuming, expensive, and labor‐intensive. In this context, biosensors offer a reliable, user‐friendly, increased assay speed, high sensitivity and require low sample volumes 7, 8…”

Section: Introductionmentioning

confidence: 99%

Biofunctionalized Nanostructured Zirconia for Biomedical Application: A Smart Approach for Oral Cancer Detection

et al. 2015

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Results of the studies are reported relating to application of the silanized nanostructured zirconia, electrophoretically deposited onto indium tin oxide (ITO) coated glass for covalent immobilization of the monoclonal antibodies (anti‐CYFRA‐21‐1). This biosensing platform has been utilized for a simple, efficient, noninvasive, and label‐free detection of oral cancer via cyclic voltammetry technique. The results of electrochemical response studies conducted on bovine serum albumin (BSA)/anti‐CYFRA‐21‐1/3‐aminopropyl triethoxy silane (APTES)/ZrO2/ITO immunoelectrode reveal that this immunoelectrode can be used to measure CYFRA‐21‐1 (oral cancer biomarker) concentration in saliva samples, with a high sensitivity of 2.2 mA mL ng−1, a linear detection range of 2–16 ng mL−1, and stability of six weeks. The results of these studies have been validated via enzyme‐linked immunosorbent assay.

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Section: Introductionmentioning

confidence: 99%

Biofunctionalized Nanostructured Zirconia for Biomedical Application: A Smart Approach for Oral Cancer Detection

et al. 2015

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“…1 The determination of cancer biomarkers is a useful task in patient diagnosis or clinical management. 2 In particular, the levels of biomarkers have been analyzed to predict the behavior and¯gure out the stage of certain cancers.…”

Section: Introductionmentioning

confidence: 99%

Two Types of Immunoassay Based on Nile Blue Labeling Polydopamine Nanospheres

Liu

et al. 2017

NANO

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The sandwich-type immunoassays have been developed by using electrochemical and surfaceenhanced Raman scattering (SERS) techniques for the detection of carcinoembryonic antigen (CEA). Nile blue as a kind of Raman dye has been decorated on nanospheres with polydopamine resin (PDR) via -stacking interaction. The Nile blue displays the strong SERS signals as well as a characteristic electrochemical reduction peak at À0.33 V (versus Ag/AgCl). The implementation of the PDR nanospheres mixing with Au nanoparticles (AuNPs/PDR) exhibits a better electrical conductivity and large SERS enhancement. The immunoassays based on Nile bluelabeled AuNPs/PDR nanospheres have been fabricated by using electrochemical and SERS techniques for the detection of CEA. The proposed immunoassay shows higher sensitivity, high selectivity, lower detection limit and long-term stability. The performances of the electrochemical immunoassay are better than that of SERS immunoassay. For the electrochemical immunoassay, the linear range occurs from 1 pg/mL to 100 ng/mL (R ¼ 0:995) with a detection limit of 0.68 pg/ mL and signal-to-noise ratio of 3 in the detection of CEA. The data for the analysis of human serum samples by using the electrochemical method are acceptably consistent with those obtained from the enzyme-linked immunosorbent assay (ELISA). The proposed immunoassay exhibits a promising potential for application in clinical diagnosis.

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“…Immunoassays of protein biomarkers constitute a very powerful tool in the early diagnosis, prognosis, and treatment of serious pathologies such as neurological disorders, cardiovascular diseases and cancer [1][2][3]. So far, label-free immunosensors based on electrochemical or piezoelectric principles have been widely reported in immunoassays related to pathologies or biomedical research fields [4][5][6].…”

Section: Introductionmentioning

confidence: 99%

Human heart failure biomarker immunosensor based on excessively tilted fiber gratings

Luo¹,

Wu²,

Zhang³

et al. 2016

Biomed. Opt. Express

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Abstract:A label-free immunosensor platform based on excessively tilted fiber gratings (ExTFGs) was developed for highly specific and fast detection of human N-terminal pro-B-type natriuretic peptide (NT-proBNP), which is considered a powerful biomarker for prognosis and risk stratification of heart failure (HF). High-purity anti-NT-proBNP monoclonal antibodies (MAbs) prepared in our laboratory were immobilized on fiber surface through the staphylococcal protein A (SPA) method for subsequent specific binding of the targeted NTproBNP. Utilizing fiber optic grating demodulation system (FOGDS), immunoassays were carried out in vitro by monitoring the resonance wavelength shift of Ex-TFG biosensor with immobilized anti-NT-proBNP MAbs. Lowest detectable concentration of ~0.5ng/mL for NTproBNP was obtained, and average sensitivity for NT-proBNP at a concentration range of 0~1.0 ng/mL was approximately 45.967 pm/(ng/mL). Several human serum samples were assessed by the proposed Ex-TFG biomarker sensor, with high specificity for NT-proBNP, indicating potential application in early diagnosing patients with acute HF symptoms. , 3023-3027 (2015).

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Lung Cancer and Its Early Detection Using Biomarker-Based Biosensors

Cited by 247 publications

References 103 publications

Biofunctionalized Nanostructured Zirconia for Biomedical Application: A Smart Approach for Oral Cancer Detection

Biofunctionalized Nanostructured Zirconia for Biomedical Application: A Smart Approach for Oral Cancer Detection

Two Types of Immunoassay Based on Nile Blue Labeling Polydopamine Nanospheres

Human heart failure biomarker immunosensor based on excessively tilted fiber gratings

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