Lung epithelial H292 cells induce differentiation of immature human HMC‐1 mast cells by interleukin‐6 and stem cell factor

Pompen, M.; Bs, Smids; Kp, Dingemans; Jansen, H. M.; Ta, Out; Lutter, René

doi:10.1046/j.1365-2222.2000.00881.x

Cited by 15 publications

(8 citation statements)

References 31 publications

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“…These findings are consistent with a report that corneal basal epithelial cells immunostain for SCF 5. These regenerated epithelial cells are an important source of a variety of cytokines, and chemotactic and growth factors, including SCF 22. Thus, our results provide more evidence that regenerated epithelial cells, indeed, contribute to the healing process.…”

Section: Discussionsupporting

confidence: 93%

Progenitor Cells in Healing after Pterygium Excision

Lee

Kim

2007

Yonsei Med J

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Bone marrow derived progenitor cells were reported to be involved in the pathogenesis of pterygium and have been suggested to be important in angiogenesis and the repair process after tissue damage. In order to investigate the involvement of these cells in wound healing after a pterygium excision, immunohistochemical staining was performed with a temporary amniotic membrane, applied to the bare sclera, after a pterygium excision using various progenitor cell markers, including CD34, c-kit, STRO-1, and AC133, to determine the expression levels of the participating cells. CD34-positive cells were observed along with some round or spindle-shaped mononuclear cells on the stromal side of the amniotic membrane. Some CD34-positive, large, and round or spindle-shaped cells formed clusters resembling small vessels in some regions of the amniotic membrane. c-kit was expressed in the epithelium that had grown over the amniotic membrane and in the spindle-shaped or round mononuclear cells in the stroma. Many stellate- to spindle-shaped fibroblast like cells expressed STRO-1, and AC133 was expressed in some round and ovoid cells. Overall, these results suggest that adult bone marrow-derived progenitor cells, such as endothelial progenitor cells and mesenchymal stem cells, are involved in the wound healing process post-excision in patients with pterygium.

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Section: Discussionsupporting

confidence: 93%

Progenitor Cells in Healing after Pterygium Excision

Lee

Kim

2007

Yonsei Med J

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“…Peripheral blood eosinophils have been used in some co-culture studies with primary nasal epithelial cells, mainly to evaluate the effect of AEC-soluble factors on eosinophil migration and activation, but also how eosinophil soluble factors affect nasal epithelium [178,179,180]. Similar studies with AEC-conditioned medium have also been used to investigate mast cell proliferation and one group has investigated mast cell adhesion to tracheal epithelial cells in a canine system [181,182]. However, to the authors' knowledge, ILC2s or basophils have yet to be used in any AEC co-culture model.…”

Section: The Accompanying Co-cultured Cellsmentioning

confidence: 99%

Polarized Airway Epithelial Models for Immunological Co-Culture Studies

Papazian

Würtzen

Hansen

2016

Int Arch Allergy Immunol

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Epithelial cells line all cavities and surfaces throughout the body and play a substantial role in maintaining tissue homeostasis. Asthma and other atopic diseases are increasing worldwide and allergic disorders are hypothesized to be a consequence of a combination of dysregulation of the epithelial response towards environmental antigens and genetic susceptibility, resulting in inflammation and T cell-derived immune responses. In vivo animal models have long been used to study immune homeostasis of the airways but are limited by species restriction and lack of exposure to a natural environment of both potential allergens and microflora. Limitations of these models prompt a need to develop new human cell-based in vitro models. A variety of co-culture systems for modelling the respiratory epithelium exist and are available to the scientific community. The models have become increasingly sophisticated and specific care needs to be taken with regard to cell types, culture medium and culture models, depending on the aim of the study. Although great strides have been made, there is still a need for further optimization, and optimally also for standardization, in order for in vitro co-culture models to become powerful tools in the discovery of key molecules dictating immunity and/or tolerance, and for understanding the complex interplay that takes place between mucosa, airway epithelium and resident or infiltrating immune cells. This review focuses on current knowledge and the advantages and limitations of the different cell types and culture methods used in co-culture models of the human airways.

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“…Human airway and nasal epithelial cells express the prosurvival and chemotactic MCspecific mediator stem cell factor, 91,92 suggesting that epithelial cells can recruit and support local MC survival. Coculture of MCs with epithelial cells results in proliferation of immature MCs, 93 increases survival of mature MCs, 91 and increases adhesion of MCs to the epithelium. 94 Subsequent MC activation by IgE/antigen in this coculture model is reduced, suggesting that in the normal, noninflamed situation epithelial cells actively suppress MC function.…”

Section: Interactions With Cells Of the Immune Systemmentioning

confidence: 99%