Lung-migrating digenean parasites: in vitro influence on nitric oxide production from normal rat pulmonary macrophages

Andrade, M. Amparo; Siles-Lucas, Mar; López‐Abán, Julio; Pérez-Arellano, José Luís; Vélez, Iván Darío; Muro, Antonio

doi:10.1016/j.exppara.2004.12.008

Cited by 5 publications

(7 citation statements)

References 15 publications

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“…Here, we investigate the possible influence of different A. suum extracts and molecules on NO production by host cells, and demonstrate that excretory/secretory (ES), but not somatic antigens from both adult worms and larvae trigger macrophage NO release in vitro . The stimulation of NO production by these antigens follows a similar pattern than that previously observed by us with antigens from other parasites (10). Thus, T. canis ES molecules trigger macrophage NO production in vitro in a dose‐dependent and specific manner (3).…”

Section: Discussionsupporting

confidence: 85%

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Antigens from Ascaris suum trigger in vitro macrophage NO production

Andrade

Siles‐Lucas

López‐Abán

et al. 2005

Parasite Immunology

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SUMMARY We investigated the in vitro effect of total excretory/secretory and somatic antigens from Ascaris suum adults (ESA and SA) and larvae 3 (ESL3 and SL3), and of 10 purified protein fractions from ESA components on rat alveolar macrophage nitric oxide (NO) production. Our results showed that in vitro incubation of macrophages with SA and SL3 antigens of A. suum did not result in NO release from cells, whereas incubation with ESA or ESL3 antigens resulted in the stimulation of NO production by these cells, both in a specific (inhibited by L-NAME and L-canavanine) and dose-dependent manner. In addition, we could demonstrate that a purified ESA fraction consisting of three Coomassie-stained bands of approximately 37, 44 and 46 kDa is involved in the in vitro triggering of NO production by host cells. These three bands were subjected to MALDI-peptide mass fingerprint, showing similarities with phosphoglycerate kinase, elongation factor Tu and enolase molecules, respectively. Future studies will focus on the characterization of these parasite-derived molecules.

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Section: Discussionsupporting

confidence: 85%

“…Alveolar macrophages were cultured as previously described (10). The cells were resuspended in Dulbecco‐modified Eagle medium (DMEM) supplemented with 10%γ‐irradiated foetal bovine serum, 2 m m glutamine and 100 U/mL−100 µg/mL penicillin–streptomycin (Sigma Chemical Co.) and plated (1 × 10 6 cells per well).…”

Section: Methodsmentioning

confidence: 99%

Antigens from Ascaris suum trigger in vitro macrophage NO production

Andrade

Siles‐Lucas

López‐Abán

et al. 2005

Parasite Immunology

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“…On the other hand, different effects were observed when macrophages were incubated with antigens from Paragonimus mexicanus and Schistosoma bovis. We have observed that excretory-secretory products from Paragonimus mexicanus adult worms trigger NO production from alveolar macrophages in vitro in a specific and concentration-dependent manner [28]. Our results also demonstrated that the stimulation of NO production by alveolar macrophages was accompanied by an increase in iNOS mRNA detection.…”

Section: Do Helminths Induce No Production By the Host Cells And supporting

confidence: 70%

“…The MALDI-peptide mass fingerprinting analysis showed similarities with two glycolytic enzymes (enolase and phosphoglycerate kinase) and with proteins involved in catalysing the elongation of peptide chains in protein biosynthesis (the elongation factor Tu ). These proteins are crucial for survival and key components of the protein synthesis machinery [28]. We also analyzed different parasite proteins to define the specific Dirofilaria immitis somatic adult-worm antigens involved in host cell NO secretion.…”

Section: Do Helminths Induce No Production By the Host Cells And mentioning

confidence: 99%

Nitric Oxide and Respiratory Helminthic Diseases

Muro

Pérez-Arellano

2010

Journal of Biomedicine and Biotechnology

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Nitric oxide (NO) is a very simple molecule that displays very important functions both in helminths (mainly those involved in respiratory pathology) and in mammalian hosts. In this paper we review four issues related to interaction of NO and lung helminthic diseases. Firstly, we evaluated data available on the NO synthesis and release by helminths and their biological role. Next, we summarized the effect of antigens obtained from different phases of the biological cycle on NO production by host mammalian cells (mainly from human sources). Thirdly, we revised the evaluation of NO on the biological activities and/or the viability of respiratory helminths. Lastly, the deleterious consequences of increased production of NO during helminthic human infection are detailed.

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“…NO plays an important role in many infectious diseases due to both its direct effector function and its potent immunoregulatory properties (6). It has been shown that excretory-secretory products from P. mexicanus adult worms trigger NO production from alveolar macrophages (1). Further studies are needed to reveal the exact effect of NO production on the host immune system.…”

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confidence: 99%

Pulmonary Paragonimiasis Diagnosed by Fine-Needle Aspiration Biopsy

et al. 2008

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We report a case of paragonimiasis involving a 12-year-old Latin American boy. The diagnosis was made by fine-needle aspiration biopsy of a pulmonary nodule. Identification of the species by morphometric analysis of the eggs indicated that the infection was caused by Paragonimus mexicanus. CASE REPORTA 12-year-old boy with a 2-year history of seizures, headaches, and intermittent hemoptysis presented to the Ben Taub General Hospital emergency room after a cluster of five seizure attacks. The boy was from El Salvador, had been in the United States for 14 months, and had been treated with phenytoin for his seizures. On presentation, his head computed tomography (CT) scan showed a calcified cystic lesion in the left anterior frontal area. The electroencephalograph was abnormal, with recurrent slow wave spikes in the left temporal lobe and a frontal slow-wave spike. He was diagnosed with symptomatic localized epilepsy with secondary generalized tonic/clonic seizures. He was started on carbamazepine (Tegretol) and followed monthly by the Neurology Service. He had been seizure free for 9 months when he presented to the emergency room for a second time with two episodes of selflimited tonic/clonic seizure at school, each lasting less than 1 minute. There was no loss of bladder or bowel control. The patient reported a 1-year history of intermittent hemoptysis. His sputum was reported to be blood streaked with no gross blood. He had an occasional productive cough but no hematemesis, nasal congestion, chest pain, or shortness of breath. He occasionally had fever but no chills or night sweats. The patient's mother reported that he had a 20-pound weight loss over the previous year. No diarrhea was reported. His physical activity was normal and he did not complain of fatigue. Headaches had been worse over the past few months; therefore, he had missed several days of school. The headaches were frontal, throbbing, and associated with photophobia. He was also complained of problems with visual acuity. He denied any syncope, vomiting, or persistent poor vision. His headaches were relieved with acetaminophen (Tylenol).His physical examination was unremarkable. His laboratory investigation documented a normal complete blood count with no eosinophilia. His metabolic panel was also normal. His cerebrospinal fluid sample had four white cells and no red cells; cerebrospinal fluid culture was negative for bacteria, fungi, and viruses. Abdominal and pelvic CT scans were normal. His chest X-ray and CT scan showed multiple lung nodules measuring up to 2.2 cm but no lymphadenopathy (Fig. 1). Pulmonary angiography showed no evidence of hypervascular tumor or arteriovenous malformation. A head CT scan revealed a focus of encephalomalacia in the left superior frontal gyrus involving the underlying white matter and a 2-to 3-mm focal calcification. A CT-guided lung biopsy using fine-needle aspiration showed abundant necrotic cellular debris surrounding numerous thick-walled parasite eggs morphologically consistent with those of the trematode or...

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Lung-migrating digenean parasites: in vitro influence on nitric oxide production from normal rat pulmonary macrophages

Cited by 5 publications

References 15 publications

Antigens from Ascaris suum trigger in vitro macrophage NO production

Antigens from Ascaris suum trigger in vitro macrophage NO production

Nitric Oxide and Respiratory Helminthic Diseases

Pulmonary Paragonimiasis Diagnosed by Fine-Needle Aspiration Biopsy

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