Lung resistance-related protein/major vault protein and vaults in multidrug-resistant cancer

Scheffer, George L.; Schroeijers, Anouk B.; Izquierdo, Miguel; Wiemer, Erik A.C.; Scheper, Rik J.

doi:10.1097/00001622-200011000-00007

Cited by 159 publications

(130 citation statements)

References 59 publications

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“…Increased levels of MVP expression have been linked to multidrug resistance (MDR) (Scheffer et al, 2000) and can be of clinical value in predicting the response of tumors to chemotherapy (Hofer and Hermann, 2001). Our studies indicate that MVP is selectively expressed compared to astrocytes and at higher frequency in glioblastomas compared to low-grade astrocytomas.…”

Section: Major Vault Proteinmentioning

confidence: 99%

Identification of differentially expressed proteins in human glioblastoma cell lines and tumors

Zhang

Tremblay

McDermid

et al. 2003

Glia

112

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KEY WORDSproteomics; matrix-assisted laser desorption ionization time-of-flight (MALDI-TOF); liquid chromatography tandem mass spectrometry (LC-MS-MS); glioblastoma; U87MG; major vault protein; cystatin B; Hsp27; tissue transglutaminase; epidermal growth factor receptor ABSTRACT An in-frame deletion of 801 bp in exons 2-7 (type III mutation) of the epidermal growth factor receptor (EGFR) is detected at high incidence in primary glioblastoma tumors. A proteomic approach was used to generate differential protein expression maps of fetal human astrocytes (FHA), human glioblastoma cell lines U87MG and U87MG expressing type III EGFR deletion (U87MG⌬EGFR) that confers high malignancy to tumor cells. Two-dimensional gel electrophoresis followed by in-gel digestion of separated spots and protein identification by LC-MS-MS and matrix-assisted laser desorption ionization time-of-flight mass spectrometry (MALDI-TOF MS) identified 23 proteins expressed at higher levels or exclusively in FHA and 29 proteins expressed at higher levels or exclusively in U87MG cells. Three proteins, ubiquitin, cystatin B, and tissue transglutaminase (TTG), were upregulated in U87MG⌬EGFR relative to U87MG. Four proteins highly expressed by U87MG cells, Hsp27, major vault protein, TTG, and cystatin B, were analyzed by Western blot, ELISA, or RT-PCR in cell extracts and in tissue samples of glioblastoma multiforme (GBM; grade IV), low-grade astrocytomas (grades I and II), and nonmalignant brain lesions. All four proteins were highly expressed in GBM tissues compared to nonmalignant brain. These proteins may be used as diagnostic or functional (e.g., multiple drug resistance, invasiveness) markers for glioblastoma tumors. GLIA 42:194 -208, 2003.

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Section: Major Vault Proteinmentioning

confidence: 99%

Identification of differentially expressed proteins in human glioblastoma cell lines and tumors

Zhang

Tremblay

McDermid

et al. 2003

Glia

112

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“…Although the exact function of vaults is not yet known, in humans, the elevated vault levels have been used to predict whether tumors will acquire multidrug resistance (reviewed in Ref. 4). They have a mass of 13 MDa, and their three-dimensional reconstruction to 22-Å resolution indicated that the inside of the barrel is spacious enough to encompass an intact ribosome, consistent with a carrier/sequestration capacity (5,6).…”

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confidence: 99%

The La RNA-binding Protein Interacts with the Vault RNA and Is a Vault-associated Protein

Kickhoefer

Poderycki

Chan

et al. 2002

Journal of Biological Chemistry

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Vaults are highly conserved ubiquitous ribonucleoprotein particles with an undefined function. Three protein species (p240/TEP1, p193/VPARP, and p100/MVP) and a small RNA comprise the 13-MDa vault particle. The expression of the unique 100-kDa major vault protein is sufficient to form the basic vault structure. Previously, we have shown that stable association of the vault RNA with the vault particle is dependent on its interaction with the p240/TEP1 protein. To identify other proteins that interact with the vault RNA, we used a UV-cross-linking assay. We find that a portion of the vault RNA is complexed with the La autoantigen in a separate smaller ribonucleoprotein particle. La interacts with the vault RNA (both in vivo and in vitro) presumably through binding to 3 -uridylates. Moreover, we also demonstrate that the La autoantigen is the 50-kDa protein that we have previously reported as a protein that co-purifies with vaults.

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“…The precise cellular function(s) of the vault complex are not yet completely clear (for reviews see Refs. [1][2][3].…”

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confidence: 99%

Multiple Human Vault RNAs

Zon¹,

Mossink²,

Schoester³

et al. 2001

Journal of Biological Chemistry

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Human vaults are intracellular ribonucleoprotein particles believed to be involved in multidrug resistance. The complex consists of a major vault protein (MVP), two minor vault proteins (VPARP and TEP1), and several small untranslated RNA molecules. Three human vault RNA genes (HVG1-3) have been described, and a fourth was found in a homology search (HVG4). In the literature only the association of hvg1 with vaults was shown in vivo. However, in a yeast three-hybrid screen the association of hvg1, hvg2, and hvg4 with TEP1 was demonstrated. In this study we investigated the expression and vault association of different vault RNAs in a variety of cell lines, including pairs of drugsensitive and drug-resistant cells. HVG1-3 are expressed in all cell lines examined, however, none of the cell lines expressed HVG4. This probably is a consequence of the absence of essential external polymerase III promoter elements. The bulk of the vault RNA associated with vaults was hvg1. Interestingly, an increased amount of hvg3 was bound to vaults isolated from multidrug-resistant cell lines. Our findings suggest that vaults bind the RNA molecules with different affinities in different situations. The ratio in which the vault RNAs are associated with vaults might be of functional importance.

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Lung resistance-related protein/major vault protein and vaults in multidrug-resistant cancer

Cited by 159 publications

References 59 publications

Identification of differentially expressed proteins in human glioblastoma cell lines and tumors

Identification of differentially expressed proteins in human glioblastoma cell lines and tumors

The La RNA-binding Protein Interacts with the Vault RNA and Is a Vault-associated Protein

Multiple Human Vault RNAs

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