Lung tissue apoptosis in abdominal hypertension

Akbulut, Gökhan; Yazıcıoğlu, Murat Burç; Şahin, Önder; Tosun, Murat; Dılek, Osman Nuri

doi:10.1007/s00068-010-0068-z

Cited by 1 publication

(2 citation statements)

References 26 publications

(41 reference statements)

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“…It means that CO 2 pneumoperitoneum in higher pressures have lethal effects on kidney tubular cells. Generally, apoptotic pathways were activated by p53 expression in our before studies [10][11][12][13][14].However, in this study, we detected that p53 expression was detected only 20 mmHg applied groups. This protection might be related with mechanical resistance of renal capsule and also retroperitoneal location of kidneys.…”

Section: Discussioncontrasting

confidence: 82%

“…However, it was revealed that the side effects of CO 2 pneumoperitoneum such as organ ischemia, cell death, physiopathological changes were minimal and reversible without any apparent sequel when the shorter time and lower pressure was used. Because we have studied the effects of CO 2 pneumoperitoneum in different intraabdominal organs in our different studies before [10][11][12][13][14], we have standardized CO 2 pressure for experimental studies.…”

Section: Discussionmentioning

confidence: 99%

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P53 related apoptosis in kidneys in CO2 pneumoperitoneum rat model: an immunohistochemical study

et al. 2014

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Laparoscopic surgery techniques have been increasingly preferred to classic laparotomy by surgeons since 1987. However, this method may have some side effects on different intraabdominal organs including kidneys. The aim of this study is to evaluate the effects of different pressures of CO2 on p53 related apoptosis in kidneys. Totally 24 male rats were divided into four equal groups. CO2 is insufflated into rats' intraabdominal cavity in two different pressures of 10 and 20 mmHg during 1 h. However, in sham group, only cannula was inserted, but no gas was insufflated. After 1 h, 30 min reperfusion was applied. At last, the kidneys were excised and p53 expression and apoptosis were evaluated immunohistochemically. All the data revealed that the number of apoptotic cell in kidney' tubular cells significantly increases in proportion to CO2 pressure level. On the other hand, p53 expression was detected only in the highest pressure. Because the low CO2 pressured group' rats had no p53 expression in kidneys, we suggest that this method can be safely used for abdominal surgery. At the same time, increasing in the number of apoptotic cells parallel to pressure also suggest that CO2 pressure level and application time are very important parameters during CO2 pneumoperitoneum.

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