Lung tissue regeneration after induced injury in Runx3 KO mice

Abstract

Runx3 is essential for normal murine lung development, and Runx3 knockout (KO) mice, which die soon after birth, exhibit alveolar hyperplasia. Wound healing, tissue repair, and regeneration mechanisms are necessary in humans for proper early lung development. Previous studies have reported that various signaling molecules, such as pErk, Tgf-beta1, CCSP, pJnk, Smad3, and HSP70 are closely related to wound healing. In order to confirm the relationship between lung defects caused by the loss of function of Runx3 and wound healing, we have localized various wound-healing markers after laser irradiation in wild-type and in Runx3 KO mouse lungs at post-natal day 1. Our results indicate that pERK, Tgf-beta1, CCSP, pJnk, and HSP70 are dramatically down-regulated by loss of Runx3 during lung wound healing. However, Smad3 is up-regulated in the Runx3 KO laser-irradiated lung region. Therefore, the lung wound-healing mechanism is inhibited in the Runx3 KO mouse, which shows abnormal lung architecture, by reduced pErk, Tgf-beta1, CCSP, pJnk, and HSP70 and by induced Smad3.

Repair after acute lung injury: molecular mechanisms and therapeutic opportunities

“…In many cases, these studies are still at the in vitro stages, such as the use of vimentin to improve wound repair [30], or just suggest a possible beneficial role (e.g., connexins [31], adrenomedullin [32] or a possible modulation of the transcription factors, FoxM1 and Runx3 [33,34]) and need further research to prove their viability in vivo . Improvement of plasma membrane repair is a possible direct treatment.…”

Repair after Acute Lung Injury: Molecular Mechanisms and Therapeutic Opportunities

“…In many cases, these studies are still at the in vitro stages, such as the use of vimentin to improve wound repair [30], or just suggest a possible benefi cial role (e.g., connexins [31], adrenomedullin [32] or a possible modulation of the transcription factors, FoxM1 and Runx3 [33,34]) and need further research to prove their viability in vivo. Improvement of plasma membrane repair is a possible direct treatment.…”

Effects of paeonol on intracellular calcium concentration and expression of RUNX3 in LoVo human colon cancer cells

“…RUNX3 is an important target of the TGF-β signaling pathway (24) and an inhibitor of the Wnt pathway (25,26). Previous studies have revealed that the central role of RUNX3 in tumor formation may be associated with its developmental significance (27,28). The major reason for the lost or decreased expression of RUNX3 is hypermethylation of the RUNX3 proximal promoter CpG island, which is involved in the formation of a number of cancer types, including breast (29), gastric (30,31) and colon cancer (32,33).…”