Lupine embryo axes under salinity stress. I. Ultrastructural response

Wojtyla, Łukasz; Kubala, Szymon; Garnczarska, Małgorzata

doi:10.1007/s11738-013-1258-1

Cited by 9 publications

(14 citation statements)

References 34 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…The fixed samples were rinsed three times with cacodylate buffer (0.05 m, pH 6.8; Polysciences), dehydrated in a graded ethanol series (10-100%), and postfixed with 1% osmium tetraoxide for 2 h at room temperature. Fixed material was counterstained, embedded in lowviscosity resin, and processed as described previously (Bagniewska-Zadworna et al, 2012;Wojtyla et al, 2013). Ultrathin sections (0.1 µm) were cut with a diamond knife on an ultramicrotome EM UC6 (Leica-Reichert, Bensheim, Germany) and collected on Formvar-coated copper grids.…”

Section: Cytology-related Analysesmentioning

confidence: 99%

Physio-Genetic Dissection of Dark-Induced Leaf Senescence and Timing Its Reversal in Barley

et al. 2018

Self Cite

View full text Add to dashboard Cite

Senescence is a ubiquitous characteristic in the biological world. From an ontogenetic perspective, senescence is now established as a developmental and genetic program acquired during evolution (Wojciechowska et al., 2018). Like in other organisms, senescence in plants is genetically programmed (Nam, 1997; van Doorn and Woltering, 2004; Wojciechowska et al., 2018). In plants, senescence is a prelude to cell (organ) death, and during this process metabolites and macromolecules released are salvaged for utilization by the plant for growth. Generally, senescence occurs prior to programmed cell death (PCD), since symptomatic leaf yellowing can be reversed based on the timing of senescence while PCD is a terminal, irreversible program. It has been suggested that the term "PCD" in plants should be restricted to the specific stage of intrinsic senescence program when it has reached a "point of no return" and leaf yellowing is no longer reversible (Mattoo and Handa, 2003). Programmed cell death in plants was described as a sequential process that included apoptosis-like necrosis and autophagy (van Doorn et al., 2011). Autophagy under normal growth conditions favors turnover of cellular components for maintaining homeostasis,

show abstract

Section: Cytology-related Analysesmentioning

confidence: 99%

Physio-Genetic Dissection of Dark-Induced Leaf Senescence and Timing Its Reversal in Barley

et al. 2018

Self Cite

View full text Add to dashboard Cite

show abstract

“…Our previous study focused on ultrastructural characterization of lupine embryo axes cells under salinity stress. Ultrastructural observations and analysis of DNA degradation confirmed the activation of PCD reaction in lupine embryo axes as a response to high salt concentration (Wojtyla et al 2013 ). According to the current knowledge of the exposure of plants to salinity, high salt concentration leads to salt shock.…”

Section: Introductionmentioning

confidence: 73%

Lupine embryo axes under salinity stress. II. Mitochondrial proteome response

2013

Self Cite

View full text Add to dashboard Cite

Germination is the first step of plant growth in plant life cycle. An embryonic radicle protruding the seed coat is the first part of plant which has direct contact with external environment including salt-affected soil. In embryo axes, mitochondria are the main energy producer. To understand better salinity impact on mitochondria functioning, this study was focused on the effect of NaCl stress onto mitochondria proteome. Mitochondria were isolated from yellow lupine (Lupine luteus L. ‘Mister’) embryo axes cultured in vitro for 12 h with 250 and 500 mM NaCl. Two-dimensional gel electrophoresis of mitochondrial proteins isolated from NaCl-treated axes demonstrated significant changes in proteins abundances as a response to salinity treatment. Twenty-one spots showing significant changes in protein expression profiles both under 250 and 500 mM NaCl treatment were selected for tandem mass spectrometry identification. This approach revealed proteins associated with different metabolic processes that represent enzymes of tricarboxylic acid cycle, mitochondrial electron transport chain, enzymes and proteins involved in mitochondria biogenesis and stresses response. Among proteins involved in mitochondria biogenesis, mitochondrial import inner membrane translocase, subunit Tim17/22, mitochondrial-processing peptidase subunit alpha-1, mitochondrial elongation factor Tu and chaperonins CPN60 were revealed. Finally, formate dehydrogenase 1 was found to accumulate in lupine embryo axes mitochondria under salinity. The functions of identified proteins are discussed in relation to salinity stress response, including salinity-induced PCD.Electronic supplementary materialThe online version of this article (doi:10.1007/s11738-013-1273-2) contains supplementary material, which is available to authorized users.

show abstract

“…Cristae organization and mitochondrial interior in salt-treated embryo axes remained as in control. Changes in mitochondria ultrastructure in lupine embryo axes during salt stress were noticed after 12 hours of culture with 0.25 and 0.5 M NaCl [13]. Oxygen consumption by mitochondria isolated from 24 h salt stressed embryo axes was higher, compared with mitochondria isolated from control embryo axes.…”

Section: Discussionmentioning

confidence: 92%

“…It is likely that these responses probably differ according to species, plant organ, developmental stage, and the severity of salinity treatment [39]. It is suggested that the ability to maintain respiratory homeostasis is linked to acquisition of salinity tolerance [39], whereas decreased rate of respiration is associated with loss of stress tolerance and could be regarded as one of the symptoms of programmed cell death [13]. Other characteristic feature observed in ultrastructure analysis is reorganization of RER.…”

Section: Discussionmentioning

confidence: 99%

“…Stress caused by salinity may also lead to PCD induction. It was shown by integrating the ultrastructural changes with observation of nuclear DNA fragmentation that strong salinity stress (0.25 and 0.5 M NaCl for 12 h) leads to PCD execution in lupine embryo axes [13]. In order to protect cells from the toxic effects of NaCl, plants have evolved different adaptive and protective mechanisms, which minimize the effects of stress and help to maintain homeostasis.…”

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

Ultrastructural and antioxidative changes in lupine embryo axes in response to salt stress

2013

Self Cite

View full text Add to dashboard Cite

Embryo axes of lupine (Lupinus luteus L. 'Mister') were subjected to 0.1 M NaCl salt stress for 24 and 48 h. The ultrastructure modification and adjustment of antioxidant enzymes activities and izoenzymes profiles were observed. In cells of lupine embryo axes grown for 48 hours in medium with 0.1 M NaCl mitochondria took the forked shape and bulges of the outer mitochondrial membranes appeared. Moreover, the inflating and swelling of rough endoplasmic reticulum (RER) lumen and fragmentation of RER were noticed. The level of H 2 O 2 was higher in salt treated embryo axes after 24 hours and increase of thiobarbituric acid reactive substances was observed after both 24 and 48 h of salt treatment. Native gel electrophoresis showed increased intensities of bands for catalase isozymes in response to salt stress, whereas activity of catalase was higher only in embryo axes grown for 48 h in control conditions. Appearance of two new isoforms of ascorbate peroxidase was observed after 48 h only under control condition, however increased activities were stated for both control and salt-stress condition after 48 h. No changes in isozymes pattern for superoxide dismutase were observed, but significant decrease in superoxide dismutase activity was noticed in relation to time and salt stress. Possible role of these enzymes in salt stress tolerance is discussed. The 0.1 M salt stress is regarded as a middle stress for lupine embryo axes and the efficiency of stress prevention mechanisms is proposed. Keywords: salt stress, lupine, ultrastructure, antioxidantJournal homepage: pbsociety.org.pl/journals/index.php/asbp ORIGINAL RESEARCH PAPER Received: 2013.04.18 Accepted: 2013.09.26 Published electronically: 2013.11.12 Acta Soc Bot Pol 82(4):303-311 DOI: 10.5586/asbp.2013 Ultrastructural and antioxidative changes in lupine embryo axes in response to salt stress Łukasz Wojtyla*, Magda Grabsztunowicz, Małgorzata Garnczarska

show abstract

Lupine embryo axes under salinity stress. I. Ultrastructural response

Cited by 9 publications

References 34 publications

Physio-Genetic Dissection of Dark-Induced Leaf Senescence and Timing Its Reversal in Barley

Physio-Genetic Dissection of Dark-Induced Leaf Senescence and Timing Its Reversal in Barley

Lupine embryo axes under salinity stress. II. Mitochondrial proteome response

Ultrastructural and antioxidative changes in lupine embryo axes in response to salt stress

Contact Info

Product

Resources

About