“…The immunological panel for SLE-associated biomarkers included: (i) the complement fractions C3, C4 (Cobas 500 ® , Roche Diagnostics GmBH, Germany) and 50 % concentration haemolytic (CH50; SpaPlus ® , The Binding Site, Birmingham, UK); (ii) IgG anti-double stranded (ds)DNA and anti-chromatin Abs (Bioplex ® , Biorad, Hercules, CA); (iii) IgG anti-extractable nuclear (ENA) Abs against sicca syndrome (SS)A 52 kDa, SSA 60 kDa, SSB, Smith (Sm), Sm-ribonucleoprotein (RNP), RNP A, and RNP 68 kDa antigens (Bioplex ® ); (iv) IgG anti-ribosomal Abs (Bioplex ® ); (v) IgG anti-C1q Abs (Quanta-lite ® , Werfen, Barcellona, Spain); and (vi) the spot urinary sCD163 (ELLA ® , Bio-techne, Minneapolis, MN) that was evaluated together with proteinuria and creatinuria (Cobas 500 ® ) in order to express sCD163 as a ratio to creatinuria, proteinuria, or not as well as the ratio of urine protein excretion to creatinine or PCR [ [22] , [23] , [24] , [25] , [26] ]. Cut-offs were fixed as recommended by the providers (C3 low <0.72 g/L; C4 low <0.11 g/L, CH50 low <31 %; anti-dsDNA Abs ≥10 international units [IU]/mL, anti-ENA/-Ribosomal/-Chromatin Abs ≥1.0 arbitrary units [AU]mL, and anti-C1q Abs ≥20 AU/mL) with the exception of the normalized urinary sCD163/creatinuria ratio as no consensus existed.…”