Luteal blood flow is a more appropriate indicator for luteal function during the bovine estrous cycle than luteal size

Herzog, Kathrin; Brockhan-Lüdemann, Maren; Kaske, Martin; Beindorff, Nicola; Paul, Vijay; Niemann, Heiner; Bollwein, Heinrich

doi:10.1016/j.theriogenology.2009.11.016

Cited by 149 publications

(169 citation statements)

References 24 publications

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“…The expression patterns of STAR and HSD3B mRNA were determined in order to validate the allocation of CLs to their respective experimental groups (early, mid-or late luteal phase). Increased expression of STAR and HSD3B between the early and mid-luteal phase, and decreased expression between the mid-and late luteal phase were consistent with changes in steroidogenic capacity of the CL throughout its lifespan (Herzog et al 2010). Determination of plasma P 4 concentrations, luteal size and blood flow further confirmed that only tissue derived from functional CLs was used.…”

Section: Discussionsupporting

confidence: 53%

LPS-mediated effects and spatio-temporal expression of TLR2 and TLR4 in the bovine corpus luteum

Lüttgenau¹,

Herzog²,

Strüve³

et al. 2016

Reproduction

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When given intravenously (iv), lipopolysaccharide (LPS) transiently suppresses the structure and function of the bovine corpus luteum (CL). This is associated with increased release of prostaglandin (PG) F 2a metabolite. The underlying regulatory mechanisms of this process remain, however, obscure. Therefore, the aims of this study were: i) to investigate the expression of the LPS receptor toll-like receptor 4 (TLR4) and 2 (TLR2) in the bovine CL during early, mid-and late luteal phases; and ii) to further dissect the mechanisms of LPS-mediated suppression of luteal function. As revealed by semi-quantitative qPCR and immunohistochemistry, both receptors were detectable throughout the luteal lifespan. Their mRNA levels increased from the early toward the mid-luteal phase; no further changes were observed thereafter. The TLR4 protein seemed more highly represented than TLR2. The cellular localization of TLRs was in blood vessels; weaker signals were observed in luteal cells. Additionally, cows were treated either with LPS (iv, 0.5 mg/kg BW) or with saline on Day 10 after ovulation. Samples were collected 1200 h after treatment and on Day 10 of the respective subsequent (untreated) cycle. The mRNA expression of several possible regulatory factors was investigated, revealing the suppression of PGF 2a receptor (PTGFR), STAR protein and 3b-hydroxysteroid dehydrogenase, compared with controls and subsequent cycles. The expression of TLR2 and TLR4, interleukin 1a (IL1A) and 1b (IL1B) and of PGF 2a and PGE 2 synthases (HSD20A and mPTGES respectively) was increased. The results demonstrate the presence of TLR2 and TLR4 in the bovine CL, and implicate their possible involvement in the deleterious effects of LPS on its function.

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Section: Discussionsupporting

confidence: 53%

LPS-mediated effects and spatio-temporal expression of TLR2 and TLR4 in the bovine corpus luteum

Lüttgenau¹,

Herzog²,

Strüve³

et al. 2016

Reproduction

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“…Various authors classify the bovine oestrous cycle into three different luteal periods on the basis of plasma progesterone levels [7]: growth (Days 4 to 7), static (8 to 16), and regression (17 to 21). Accordingly to other authors [1], we divided the oestrous cycle into four phases; in particular, the above reported static phase was subdivided into mid and late.…”

Section: Resultsmentioning

confidence: 99%

Immunopresence and functional activity of prostaglandin-endoperoxide synthases and nitric oxide synthases in bovine corpora lutea during diestrus

et al. 2013

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“…Mean diameters and calculated cross-sectional areas of the CL ranged from 25.5 to 33.0 mm and 5.11 to 8.55 cm 2 , respectively, with the exception of only one CL with a diameter and area of 22.5 mm and 3.98 cm 2 , respectively (for the latter CL, P 4 concentration in a blood sample collected immediately before slaughter was 7.2 ng/ml). Based on cross-sectional areas (nZ10) or plasma P 4 concentration (nZ1), CL were designated as mid-cycle (Days 8-16; Day 1Zovulation) according to Herzog et al (2010).…”

Section: Discussionmentioning

confidence: 99%

Lipopolysaccharide enhances apoptosis of corpus luteum in isolated perfused bovine ovaries in vitro

Lüttgenau¹,

Möller²,

Kradolfer³

et al. 2016

Reproduction

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Lipopolysaccharide (LPS), the endotoxin of Gram-negative bacteria, has detrimental effects on the structure and function of bovine corpus luteum (CL) in vivo. The objective was to investigate whether these effects were mediated directly by LPS or via LPS-induced release of PGF 2a . Bovine ovaries with a mid-cycle CL were collected immediately after slaughter and isolated perfused for 240 min. After 60 min of equilibration, LPS (0.5 mg/ml) was added to the medium of five ovaries, whereas an additional six ovaries were not treated with LPS (control). After 210 min of perfusion, all ovaries were treated with 500 iu of hCG. In the effluent perfusate, concentrations of progesterone (P 4 ) and PGF 2a were measured every 10 and 30 min, respectively. Punch biopsies of the CL were collected every 60 min and used for RT-qPCR to evaluate mRNA expression of receptors for LPS (TLR2, -4) and LH (LHCGR); the cytokine TNFA; steroidogenic (STAR, HSD3B), angiogenic (VEGFA 121 , FGF2), and vasoactive (EDN1) factors; and factors of prostaglandin synthesis (PGES, PGFS, PTGFR) and apoptosis (CASP3, -8, -9). Treatment with LPS abolished the hCG-induced increase in P 4 (P%0.05); however, there was a tendency (PZ0.10) for increased release of PGF 2a at 70 min after LPS challenge. Furthermore, mRNA abundance of TLR2, TNFA, CASP3, CASP8, PGES, PGFS, and VEGFA 121 increased (P%0.05) after LPS treatment, whereas all other factors remained unchanged (PO0.05).In conclusion, reduced P 4 responsiveness to hCG in LPS-treated ovaries in vitro was not due to reduced steroidogenesis, but was attributed to enhanced apoptosis. However, an impact of luteal PGF 2a could not be excluded.

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Luteal blood flow is a more appropriate indicator for luteal function during the bovine estrous cycle than luteal size

Cited by 149 publications

References 24 publications

LPS-mediated effects and spatio-temporal expression of TLR2 and TLR4 in the bovine corpus luteum

LPS-mediated effects and spatio-temporal expression of TLR2 and TLR4 in the bovine corpus luteum

Immunopresence and functional activity of prostaglandin-endoperoxide synthases and nitric oxide synthases in bovine corpora lutea during diestrus

Lipopolysaccharide enhances apoptosis of corpus luteum in isolated perfused bovine ovaries in vitro

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