Luteal toxicity evaluation in rats

Taketa, Yoshikazu

doi:10.1293/tox.2021-0058

Cited by 4 publications

(2 citation statements)

References 47 publications

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“…Follicles were classified as cystic based on criteria given by Lara et al [ 30 ]. CL were classified as CL from the present and CL from the previous cycles (presence of fibrous tissue formation and necrosis), according to criteria given by Taketa [ 31 ] and Sato et al [ 32 ]. Only large CL (above 800 µm in diameter) were counted.…”

Section: Methodsmentioning

confidence: 99%

Rat Ovarian Function Is Impaired during Experimental Autoimmune Encephalomyelitis

Milosevic

Lavrnja

Savić

et al. 2023

Cells

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Multiple sclerosis (MS) is an autoimmune disease affecting the CNS and occurring far more prevalently in women than in men. In both MS and its animal models, sex hormones play important immunomodulatory roles. We have previously shown that experimental autoimmune encephalomyelitis (EAE) affects the hypothalamic–pituitary–gonadal axis in rats of both sexes and induces an arrest in the estrous cycle in females. To investigate the gonadal status in female rats with EAE, we explored ovarian morphometric parameters, circulating and intraovarian sex steroid levels, and the expression of steroidogenic machinery components in the ovarian tissue. A prolonged state of diestrus was recorded during the peak of EAE, with maintenance of the corpora lutea, elevated intraovarian progesterone levels, and increased gene and protein expression of StAR, similar to the state of pseudopregnancy. The decrease in CYP17A1 protein expression was followed by a decrease in ovarian testosterone and estradiol levels. On the contrary, serum testosterone levels were slightly increased. With unchanged serum estradiol levels, these results point at extra-gonadal sites of sex steroid biosynthesis and catabolism as important regulators of their circulating levels. Our study suggests alterations in the function of the female reproductive system during central autoimmunity and highlights the bidirectional relationships between hormonal status and EAE.

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Section: Methodsmentioning

confidence: 99%

Rat Ovarian Function Is Impaired during Experimental Autoimmune Encephalomyelitis

Milosevic

Lavrnja

Savić

et al. 2023

Cells

View full text Add to dashboard Cite

show abstract

“…Atretic follicles were identified by degenerated oocytes with pyknosis in granulosa cells and granulosa cell debris in the antral cavity [ 34 ]. Corpus luteum is identified by when one of the following observations, such as basophilic cytoplasm, larger size with polygonal and thinly vacuolated luteal cells, apoptosis of luteal cells, and vacuolation, and increased cytoplasmic fibrous tissue and decreased vacuolation [ 35 ]. Cystic follicles were identified by cystic follicles with an inner thickened theca cell layer and irregular thickness of granulosa cells [ 36 ].…”

Section: Collection and Authentication Of Plant Materialsmentioning

confidence: 99%