Luteimonas saliphila sp. nov. and Luteimonas salinisoli sp. nov., two novel strains isolated from saline soils

Abstract

Two Gram-stain-negative, motile with single polar flagellum, rod-shaped bacterial strains, named SJ-9T and SJ-92T, were isolated from saline soils from Inner Mongolia, PR China. SJ-9T and SJ-92T grew at pH 6.5–10.0 and 7.0–11.0, 10–35 °C, and in the presence of 0–5 % and 0–8 % NaCl, respectively. Both strains were positive for oxidase, and negative for catalase. The results of phylogenetic analysis based on 16S rRNA gene sequences indicated that SJ-9T clustered with Luteimonas marina FR1330T (sharing 97.9 % 16S rRNA gene similarity), Luteimonas huabeiensis HB2T (96.5 %), ‘Luteimonas wenzhouensis’ YD-1 (96.6 %), and Luteimonas composti CC-YY255T (95.1 %), and shared low 16S rRNA gene similarities (<97.0 %) with all the other type strains; while SJ-92T clustered with Luteimonas aestuarii B9T (98.2 %), and shared low 16S rRNA gene similarities (<98.0 %) with all the other type strains. The two strains shared 97.4 % 16S rRNA gene similarity with each other. The major cellular fatty acids of both strains are iso-C15 : 0 and summed feature 9 (C16 : 0 10-methyl and/or iso-C17 : 1ω9c). The major polar lipids of both strains are diphosphatidylglycerol, phosphatidylglycerol and phosphatidylethanolamine. The only respiratory quinone for both strains is ubiquinone-8 (Q-8). The genomic DNA G+C contents are 69.3 and 70.4 mol%, respectively. The digital DNA–DNA hybridization (dDDH) and average nucleotide identity by blast (ANIb) values between the two strains were 22.6 and 77.5 %, while the values between SJ-9T and ‘L. wenzhouensis’ YD-1, L. marina FR1330T, and L. huabeiensis HB2T were 38.1, 39.2, and 21.9 %, and 82.5, 84.4, and 78.5 %, while those between SJ-92T and L. aestuarii B9T were 21.3 and 76.7 %. On the basis of the phenotypic, physiological and phylogenetic results, SJ-9T and SJ-92T represent two novel species of the genus Luteimonas , for which the names Luteimonas saliphila [type stain SJ-9T (=CGMCC 1.17377T=KCTC 82248T)] and Luteimonas salinisoli [type strain SJ-92T (=CGMCC 1.17695T=KCTC 82208T)] are proposed.

Ruania suaedae sp. nov. and Ruania halotolerans sp. nov., two actinobacteria isolated from saline soil, and reclassification of Haloactinobacterium kanbiaonis as Occultella kanbiaonis comb. nov.

“…Saline-alkaline soils cover a wide area of the planet and harbour considerable bacterial diversity with many new species described recently [6,[9][10][11][12][13][14][15]. During a survey of the cultivated bacterial diversity in saline soil in northern China, two strains with high 16S rRNA gene similarities to Ruania strains were isolated from saline soil.…”

Three novel Luteimonas species from a root and rhizosphere soil of Kalidium cuspidatum: Luteimonas endophytica sp. nov., Luteimonas rhizosphaericola sp. nov. and Luteimonas kalidii sp. nov.

“…gen. n. kalidii of the plant Kalidium cuspidatum). *Data for reference strains were compiled from the literature [5,7,14,58,59]. †These data were calculated from the whole genome sequence.…”

“…viscosa' CGMCC 1.12158 T ; 6, L. wenzhouensis YD-1 T ; 7, L. marina KCTC 12327 T ; 8, L. composti DSM 19790 T ; 9, L. salinisoli SJ-92 T . Data were from this study and our previous research [7] except where indicated otherwise. All the strains are positive for arginine dihydrolase, hydrolysis of aesculin and assimilaton of maltose, fructose and esterase lipase (C8), and negative for glucose fermentation, sunflower acid, citric acid, phenylacetic acid and β-fucosidase.…”

“…The Luteimonas strains usually are aerobic, heterotrophic, Gram-stain-negative, yellow-coloured bacteria with summed feature 9 and iso-C 15 : 0 as the predominant fatty acids, ubiquinone-8 (Q-8) as the major respiratory quinone, and genomic DNA G+C contents of 64.3-73.4 mol% [4]. The Luteimonas strains were isolated from various habitants, such as sea water [5], soil [4,[6][7][8][9][10], rhizosphere soil [11,12], sediment [13,14], fresh water [15], food waste [16], plant [17] and intertidal macroalga [18]. Some Luteimonas species not only play a significant role in the ecosystem, but also have a potential value in the field of basic research and biological engineering.…”