Luteimonas tolerans sp. nov., isolated from hexachlorocyclohexane-contaminated soil

Abstract

A Gram-stain-negative, aerobic, rod-shaped, non-spore-forming, yellow pigmented bacterial strain (UM1 T ) was isolated from the hexachlorocyclohexane (HCH)-contaminated dumpsite located at Ummari village in Lucknow, India. Phylogenetic analysis based on 16S rRNA gene sequences revealed that strain UM1 T belongs to the genus Luteimonas with Luteimonas aestuarii B9 T as the closest neighbour (97.2 % 16S rRNA gene sequence similarity). The DNA G+C content of strain UM1 T was 64.3 mol%. The major polar lipids were diphosphatidylglycerol (DPG), phosphatidylethanolamine (PE) and phosphatidylglycerol (PG). Main fatty acids were iso-C 15 : 0 , iso-C 11 : 0 , iso-C 11 : 0 3-OH, iso-C 17 : 0 and summed feature 9 (C 16 : 0 10-methyl and/or iso-C 17 : 1 v9c). Ubiquinone (Q-8) was the only respiratory quinone. Spermidine was detected as the major polyamine. The DNA-DNA relatedness value of strain UM1 T with respect to its closest neighbour Luteimonas aestuarii B9 T was well below 70 % (,49 %). Thus, data obtained from phylogenetic analysis, DNA-DNA hybridization, and chemotaxonomical and biochemical analyses supports classification of strain UM1 T as representative of a novel species of the genus Luteimonas, for which the name Luteimonas tolerans sp. nov. is proposed. The type strain is UM1 T (5DSM 28473 T 5MCC 2572 T 5KCTC 42936 T ).

Defining the Environmental Adaptations of Genus Devosia: Insights into its Expansive Short Peptide Transport System and Positively Selected Genes

“…In an effort to characterize the culturable diversity of soil contaminated with HCH [12][13][14][15][16][17][18] , we isolated and characterized four novel members of the genus Devosia viz. D. chinhatensis IPL18 7 , D. crocina IPL20 8 , D. albogilva IPL15 8 and D. lucknowensis L15 19 .…”

Luteimonas gilva sp. nov., isolated from farmland soil

“…The genus currently comprises 19 recognized species with validly published names. These members have been isolated from various environments such as sea water [2], fresh water [3], stratum water [4], soil [5,6], hexachlorocyclohexane-contaminated soil [7], seashore sediment [8], deep-sea sediment [9,10], food waste [11] and rhizosphere soil [12,13,14]. Cells of the Luteimonas strains are described as being Gram-negative, yellow-coloured and rod-shaped.…”

Three novel Luteimonas species from a root and rhizosphere soil of Kalidium cuspidatum: Luteimonas endophytica sp. nov., Luteimonas rhizosphaericola sp. nov. and Luteimonas kalidii sp. nov.

“…The Luteimonas strains usually are aerobic, heterotrophic, Gram-stain-negative, yellow-coloured bacteria with summed feature 9 and iso-C 15 : 0 as the predominant fatty acids, ubiquinone-8 (Q-8) as the major respiratory quinone, and genomic DNA G+C contents of 64.3-73.4 mol% [4]. The Luteimonas strains were isolated from various habitants, such as sea water [5], soil [4,[6][7][8][9][10], rhizosphere soil [11,12], sediment [13,14], fresh water [15], food waste [16], plant [17] and intertidal macroalga [18]. Some Luteimonas species not only play a significant role in the ecosystem, but also have a potential value in the field of basic research and biological engineering.…”