Lutein plus Water Chestnut (Trapa bispinosa Roxb.) Extract Inhibits the Development of Cataracts and Induces Antioxidant Gene Expression in Lens Epithelial Cells

Abstract

Age-related cataract (ARC) is the major cause of blindness worldwide. The most significant factors are the maximal exposure of the eye lens to environmental stressors, including oxidative and glycative load. The administration of antioxidant and antiglycative supplements may reduce the risk of cataract progression. In this study, the effects of lutein (LU) and water chestnut (Trapa bispinosa Roxb.) extract (TBE) on cataracts and the expression of antioxidant-related genes were assessed in Shumiya cataract rats (SCRs). LU+TBE or castor oil (COil) as a control was administered to 6- or 9-week-old cataractous SCRs and noncataractous SCRs via a feeding needle for 3 or 4 weeks. Five-week-old SCRs were provided ad libitum access to solid regular chow containing LU, TBE, LU+TBE, or the same chow without LU and/or TBE for 3 weeks. Lenses from all rats were then extracted and photographed. The right eyes of the rats were processed for histological observation, and the left eyes were used for total RNA extraction from lens epithelial cells (LEC). The mRNA levels of antioxidant proteins, peroxiredoxin 6, and catalase were examined using real-time quantitative polymerase chain reaction. Lens opacity appeared in all cataractous SCRs that began receiving LU+TBE at 9 weeks of age. However, compared to the COil group, lens opacity was decreased in the cataractous LU+TBE SCRs in all experiments. The mRNA expression levels of peroxiredoxin 6 and catalase in LECs of cataractous SCRs and cultured human LECs increased after the administration of LU+TBE. Collectively, our results highlight the anticataract and antioxidative effects of LT+TBE in SCRs. LT+TBE supplementation may, thus, be useful in delaying cataract progression.

Effect of a Lens Protein in Low-Temperature Culture of Novel Immortalized Human Lens Epithelial Cells (iHLEC-NY2)

“…The relative humidity was set at 50% considering the typical ambient condition. The effect of relative humidity is not high in a resting condition [ 23 , 24 ]. For test subjects exposed to solar radiation, an additional heat load obtained from the electromagnetic computation was applied.…”

Identification of Differential Gene Expression Pattern in Lens Epithelial Cells Derived from Cataractous and Noncataractous Lenses of Shumiya Cataract Rat

“…Cat+ and Cat- SCRs were distinguished via PCR using genomic DNA isolated from the tails of 4-week-old rats. The amplified products were then separated on a 15% gel via polyacrylamide gel electrophoresis (PAGE) to detect Lss mutation ( Lss s / Lss s or Lss s /Lss l ) as reported in a previous study of ours [ 20 ]. The sequences of primers used to detect Lss mutation were as follows: 5′-GCACACTGGACTGTGGCTGG-3′ and 5′-GCCACAGCATTGTAGAGTCGCT-3′.…”

Potensi Ekstrak Jagung Sebagai Pencegahan Katarak

“…1, November 2022, hlm. [14][15][16][17][18][19][20] e-ISSN 2715-4432 PUSLITBANG Sinergis Asa Professional, Jember pembedahan sesegera mungkin. Komplikasi serius lainnya dapat disebabkan oleh waktu operasi yang tertunda.…”

“…Hasil menunjukkan bahwa lutein memiliki peran potensial dalam mengurangi risiko katarak. Fungsi antioksidan 20 Lutein memainkan peran antioksidan kunci di retina dan dengan demikian melindungi mata dari peradangan dan sampai saat ini, sebagian besar data telah diperoleh pada fungsi pelindung lutein dalam kesehatan mata dalam kaitannya dengan degenerasi makula terkait usia.…”