Luteinizing hormone and follicle stimulating hormone induce premature condensation of chromatin in goat (Capra hircus) oocytes

Kumar, Jitendra; Osborn, J. C.; Cameron, A. W. N.

doi:10.1071/rd9910585

Cited by 10 publications

(7 citation statements)

References 19 publications

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“…There is some evidence to support this hypothesis. First, we have shown that increasing the level of LH bioactivity during superovulation of goats with FSH, through the administration of subovulatory doses of hCG, resulted in chromatin condensation without nuclear membrane break¬ down (Kumar et al, 1991). Second, Bomsel-Helmreich et al (1989) have found that low doses of hCG initiated chromatin condensation and not germinal vesicle breakdown in rabbit oocytes, whereas with increasing doses of hCG, more advanced stages of nuclear maturation occurred.…”

Section: Discussionmentioning

confidence: 91%

Follicular steroidogenesis and oocyte maturation after superovulation of goats (Capra hircus) with gonadotrophins

Kumar

Osborn²,

Cameron³

et al. 1992

Reproduction

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Follicles were sampled at three different times after treatment with 1200 iu pregnant mares' serum gonadotrophin (PMSG) or 12 mg ovine follicle-stimulating hormone (FSH), and from untreated control animals. The meiotic status and protein synthesis of the oocyte from each follicle was determined and correlated with the intrafollicular concentration of oestradiol and progesterone. Significantly higher amounts of oestradiol were present in PMSG-treated animals at sponge withdrawal than in FSH-treated and control goats. Twenty hours later, both oestradiol and progesterone concentrations in the PMSG group were higher than those in the FSH group, and were equivalent to control animals at the onset of oestrus. At 18 h after the administration of human chorionic gonadotrophin (hCG), oestradiol decreased markedly in all three treatment groups, whereas progesterone remained significantly higher in PMSG-treated follicles. Although these high concentrations of intrafollicular steroids were associated with a higher incidence of premature condensation of chromatin in oocytes, the two events were not causally related. Moreover, cytoplasmic maturation was not prematurely activated in these oocytes and a changed pattern of protein synthesis was observed in oocytes from all three treatment groups after the hCG injection. Whereas disturbances in follicular steroidogenesis of oestradiol and progesterone occur in vivo in goats superovulated with PMSG, they do not underlie the premature activation of the initial stages of nuclear maturation in oocytes but are associated with normal cytoplasmic maturation.

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Section: Discussionmentioning

confidence: 91%

Follicular steroidogenesis and oocyte maturation after superovulation of goats (Capra hircus) with gonadotrophins

Kumar

Osborn²,

Cameron³

et al. 1992

Reproduction

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“…It implies that the superovulation effect was not boosted up by high and low dosages of exogenous pFSH. In superovulated does, reduced fertility occurred due to prolonged elevation in estrogen concentrations resulting from the large anovulatory follicles and the abnormal maturation of oocytes (Cameron et al, 1988; Kumar et al, 1991; Ryan et al, 1991). Moreover, high estradiol concentration around and/or beyond ovulation induced disorders on the later reproductive process of fertilization and embryo development (Veiga-Lopez et al, 2006).…”

Section: Discussionmentioning

confidence: 99%

Follicle Stimulating Hormone (FSH) Dosage Based on Body Weight Enhances Ovulatory Responses and Subsequent Embryo Production in Goats

Rahman

Khadijah

Abdullah

2014

Asian Australas. J. Anim. Sci

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An experiment was conducted to evaluate the efficacy of porcine follicle stimulating hormone (pFSH) dosage based on body weight (BW) on ovarian responses of crossbred does. Thirty donor does were divided into 3 groups getting pFSH dosages of 3, 5, and 8 mg pFSH per kg BW, respectively, and were named as pFSH-3, pFSH-5 and pFSH-8, respectively. Estrus was synchronized by inserting a controlled internal drug release (CIDR) device and a single injection of prostaglandin F2α (PGF2α). The pFSH treatments were administered twice a day through 6 decreasing dosages (25, 25, 15, 15, 10, and 10% of total pFSH amount; decreasing daily). Ovarian responses were evaluated on Day 7 after CIDR removal. After CIDR removal, estrus was observed 3 times in a day and pFSH treatments were initiated at 2 days before the CIDR removal. All does in pFSH-5 and pFSH-8 showed estrus signs while half of the does in pFSH-3 showed estrus signs. No differences (p>0.05) were observed on the corpus luteum and total ovarian stimulation among the treatment groups, while total and transferable embryos were higher (p<0.05) in pFSH-5 (7.00 and 6.71) than pFSH-3 (3.00 and 2.80) and pFSH-8 (2.00 and 1.50), respectively. In conclusion, 5 mg pFSH per kg BW dosage gave a higher number of embryos than 3 and 8 mg pFSH per kg BW dosages. The results indicated that the dosage of pFSH based on BW is an important consideration for superovulation in goats.

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“…Furthermore, unfertilised ova may also be attributed to the abnormal maturation of oocytes, due to premature activation of the initial stages of meiotic oocyte maturation. This abnormality usually occurs as a result of increased blood LH concentrations following eCG superovulation treatment -as this hormone exhibits both LH and FSH bioactivity (Cameron et al, 1988 andKumar et al, 1991).…”

Section: Discussionmentioning

confidence: 99%

Preliminary results evaluating a simplified superovulation protocol in Boer goats

Lehloenya

2013

Small Ruminant Research

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This study was conducted to evaluate the use of one shot follicle stimulating hormone (FSH) plus equine chorionic gonadotrophin (eCG) (simplified) superovulation protocol in mature Boer goat does. Eighteen does used were allocated into two groups, during the natural breeding season (autumn). The two group were Group 1: the control (n = 9); multiple FSH protocol and Group 2: treatment group (n = 9); one shot FSH plus eCG protocol. All does were synchronised for oestrus, using controlled internal drug release dispensers (CIDR's) for 9 days. Does in the multiple FSH protocol group were superovulated with 200 μg pFSH/doe, administered i.m. in 7 dosages at 12 h intervals, starting 48 h prior to CIDR removal. In the treatment group (one shot FSH plus eCG protocol), the superovulation treatment was administered once as 200 μg FSH plus 350 IU eCG, 48 h prior to CIDR removal. All does were then observed for oestrous behaviour twice daily, at 12 h intervals following CIDR removal for a period of 72 h. Cervical inseminations (with 0.1 ml fresh undiluted Boer goat semen) were performed 36 h and 48 h following CIDR removal. Embryos were then surgically flushed on day 6 following the second artificial insemination. The time interval from CIDR removal to onset of oestrus was not affected by treatment. The duration of the oestrous period however was significantly longer in the one shot group, compared to the multiple FSH protocol. Does from the one shot FSH plus eCG protocol recorded a significantly (p < 0.05) lower mean number of CL's, structures (unfertilised ova plus embryos) and (p < 0.01) embryos recovered. A significantly (p < 0.01) higher number of unfertilised ova were observed from the one shot FSH plus eCG group (4.06 ± 0.9), compared to the multiple FSH group (0.24 ± 0.1). However, the number of degenerated embryos was not affected by treatment. The total number of transferable embryos was significantly lower in the one shot FSH plus eCG protocol (0.22 ± 0.2), compared to the multiple FSH protocol (9.56 ± 1.9). The size of follicles was not affected by treatment, however the total number of follicles at CIDR removal was significantly lower in the one shot FSH plus eCG group (11.67 ± 0.7), compared to the multiple FSH group (14.83 ± 0.7). AI might have been performed at the wrong time, because of the duration of the oestrous period being longer in Group 2. The multiple FSH superovulation protocol overall seemed to be more efficient in the Boer goat. More research is needed on the doses of gonadotrophins administered and the time of AI, in order for the simplified protocol (Group 2) to be effective.

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Luteinizing hormone and follicle stimulating hormone induce premature condensation of chromatin in goat (Capra hircus) oocytes

Cited by 10 publications

References 19 publications

Follicular steroidogenesis and oocyte maturation after superovulation of goats (Capra hircus) with gonadotrophins

Follicular steroidogenesis and oocyte maturation after superovulation of goats (Capra hircus) with gonadotrophins

Follicle Stimulating Hormone (FSH) Dosage Based on Body Weight Enhances Ovulatory Responses and Subsequent Embryo Production in Goats

Preliminary results evaluating a simplified superovulation protocol in Boer goats

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