Luteinizing Hormone Effect on Luteal Cells Is Dependent on the Corpus Luteum Stage in Felids

Hryciuk, Michał M; Jewgenow, Katarina; Braun, Beate C.

doi:10.3390/ani11010179

Cited by 4 publications

(2 citation statements)

References 39 publications

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“…Experiment 2: to study the effect of PNX-14 on steroidogenesis, cells were treated with PNX-14 at 1, 10, 100, or 1000 nM and 100 ng/mL of LH alone or with 10 nM of PNX-14. For steroidogenesis, the effect of PNX-14 (10 nM) induced by LH was studied; LH is a well-known stimulator of steroid synthesis in luteal cells [ 26 ]. The PNX-14 doses were chosen based on previous studies concerning the effect of PNX on ovarian physiology [ 7 , 10 ] and our preliminary data.…”

Section: Methodsmentioning

confidence: 99%

Phoenixin-14 as a novel direct regulator of porcine luteal cell functions

Mlyczyńska,

Kurowska,

Wachowska

et al. 2023

Biology of Reproduction

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Phoenixin is a neuropeptide with a well-established role in the central regulation of reproductive processes; however, knowledge regarding its role in the ovary is limited. One of the main active phoenixin isoforms is phoenixin-14, which acts through G protein–coupled receptor 173. Our research hypothesis was that phoenixin-14 is expressed in porcine corpus luteum and exerts luteotropic action by affecting the endocrine function of luteal cells through G protein–coupled receptor 173 and protein kinase signaling. Luteal cells were cultured to investigate the effect of phoenixin-14 (1–1000 nM) on endocrine function. We showed that phoenixin-14 and G protein–coupled receptor 173 are produced locally in porcine corpus luteum and their levels change during the estrous cycle. We detected phoenixin-14 immunostaining in the cytoplasm and G protein–coupled receptor 173 in the cell membrane. Plasma phoenixin levels were highest during the early luteal phase. Interestingly, insulin, luteinizing hormone, progesterone, and prostaglandins decreased phoenixin-14 levels in luteal cells. Phoenixin-14 increased progesterone, estradiol, and prostaglandin E2 secretion, but decreased prostaglandin F2α, upregulated the expression of steroidogenic enzymes, and downregulated receptors for luteinizing hormone and prostaglandin. Also, phoenixin-14 increased the expression of G protein–coupled receptor 173 and the phosphorylation of extracellular signal-regulated kinase 1/2, protein kinase B, inhibited the phosphorylation of protein kinase A, and had mixed effect on AMP-activated protein kinase alpha and protein kinase C. G protein–coupled receptor 173 and extracellular signal-regulated kinase 1/2 mediated the effect of phoenixin-14 on endocrine function of luteal cells. Our results suggest that phoenixin is produced by porcine luteal cells and can be a new regulator of their function.

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Section: Methodsmentioning

confidence: 99%

Phoenixin-14 as a novel direct regulator of porcine luteal cell functions

Mlyczyńska,

Kurowska,

Wachowska

et al. 2023

Biology of Reproduction

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“…Reproductive cycle of the animals play important role in productivity of the animals in terms of milk yield and meat. Earlier attempts were focused on the control of follicle growth and luteal span (Hryciuk et al, 2021;Channo et al, 2022). There are two methods of manipulating corpus luteum (CL) using either Progestins or injecting with luteolytic agents.…”

Section: Introductionmentioning

confidence: 99%

Comparative Efficacy of Medroxyprogesterone Acetate (Progestin) Sponges with Co-Synch Protocol for Estrus Synchronization in Holstein Friesian Cattle

BABAN ALI,

ASMATULLAH KAKA,

AKEEL AHMED MEMON

et al. 2023

USJAS

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The study involved forty non-pregnant, first-parity Holstein Friesian cows housed under semi-intensive management conditions. They were divided into four groups: Group A received a 7-day CIDR followed by a PGF2α injection, Group B received a 12-day CIDR followed by a PGF2α injection, Group C underwent a 10-day Co-Synch protocol, and Group D served as the control. Estrus symptoms were weak in all groups, while strong signs of standing heat were observed. Group B showed the highest estrus response, earliest onset of estrus, longest duration, and highest conception rate, resulting in positive pregnancies. In contrast, Group D had no pregnancies. These findings suggest that the extended CIDR treatment in Group B led to improved reproductive outcomes, indicating its potential as an effective method for estrus synchronization in Holstein Friesian cattle.

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Development of a 3D in vitro model to study corpus luteum of felids based on luteinized cells from antral follicles

Hryciuk,

Schröter,

Claaßen

et al. 2024

Cell Tissue Res

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The study aimed to establish a long-term 3D cell culture model using luteinized follicular cells to investigate the functionality and life cycle of the CL in felids. A mixture of cell types from antral follicles was luteinized in vitro and cultured for up to 23 days. The method, initially applied to the domestic cat, was later extended to Persian and Clouded leopards. Antral follicles were isolated and digested with enzymes; then, the cells were subjected to culture. Experimental subsets were treated with/without 1 µg/mL cloprostenol to validate the cell culture model’s suitability for functional studies. In domestic cat samples, microscopic evaluation indicated luteinization, which was confirmed by increased progestagen concentrations and IHC staining for HSD3B and CYP11A1. The gene expression of selected steroidogenic factors (HSD3B1, STAR, CYP11A1) and hormone receptors (LHCGR, PTGFR, PRLR) significantly increased, while CYP17A1 expression decreased. Cloprostenol treatment resulted in reduction of steroidogenic activity, proving its suitability for functional studies. Persian and Clouded leopards’ cell cultures exhibited similar patterns in progestagen secretion and gene expression, compared to domestic cats. This model, with its defined luteinization, as well as high and stable progestagen production, allows future investigation of factors regulating CL life cycle and function.

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Luteinizing Hormone Effect on Luteal Cells Is Dependent on the Corpus Luteum Stage in Felids

Cited by 4 publications

References 39 publications

Phoenixin-14 as a novel direct regulator of porcine luteal cell functions

Phoenixin-14 as a novel direct regulator of porcine luteal cell functions

Comparative Efficacy of Medroxyprogesterone Acetate (Progestin) Sponges with Co-Synch Protocol for Estrus Synchronization in Holstein Friesian Cattle

Development of a 3D in vitro model to study corpus luteum of felids based on luteinized cells from antral follicles

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