Luteinizing Hormone-Releasing Hormone Neurons Express c-<i>fos</i>Antigen after Steroid Activation*

Hoffman, Gloria E.; Lee, W.-S.; Attardi, Barbara; Yann, V.; Fitzsimmons, Mark D.

doi:10.1210/endo-126-3-1736

Cited by 199 publications

(108 citation statements)

References 11 publications

Supporting

Mentioning

100

Contrasting

Order By: Relevance

“…In the spinal cord, noxious and electrical stimuli induce c-fos gene expression in some dorsal horn neurones (Hunt, Pini & Evans, 1987;Wisden et al 1990); in the hippocampus, administration of convulsants leads to induction to c-fos (Morgan, Cohen, Hempstead & Curran, 1987;Kaczmark et at. 1988); in the paraventricular nucleus, adrenalectomy leads to an increase in c-fos protein (Jacobson, Sharp & Dallman, 1990); and luteinizing hormone-releasing hormone (LHRH) neurones in the preoptic area show increased immunoreactive c-fos after estrogen treatment (Hoffman, Lee, Attardi, Yann & Fitzsimmons, 1990). Physiological stimuli will also result in activation of c-fos: in the suprachiasmatic nucleus, photic stimulation results in increased levels of c-fos mRNA (Kornhauser et at.…”

Section: C-fos Mrna In Oxytocin Neurones Discussionmentioning

confidence: 99%

Electrical activation and c‐fos mRNA expression in rat neurosecretory neurones after systemic administration of cholecystokinin.

Hamamura

Leng

Emson

et al. 1991

The Journal of Physiology

View full text Add to dashboard Cite

SUMMARY1. The expression of c-fos mRNA in the rat hypothalamus was examined by in situ hybridization following systemic administration of cholecystokinin (CCK), a procedure known to activate magnocellular oxytocin neurones but not magnocellular vasopressin neurones.2. Conscious male rats were given a single i.P. injection of 50 jug/kg CCK. c-fos mRNA signal was apparent in the supraoptic and paraventricular nuclei in rats killed 10 min after injection but not in uninjected or saline-(vehicle) injected rats. The density of c-fos mRNA at both sites was further elevated in rats killed 30 min or 60 min following injection, and was absent in rats killed 4 h after injection.3. In the paraventricular nucleus the most dense expression of c-fos mRNA following CCK administration was in the medial, mainly parvocellular portion of the nucleus, in an area corresponding to the distribution ofcorticotrophin-releasing factor mRNA determined by in situ hybridization in adjacent sections.4. The i.P. injection of CCK increased plasma oxytocin concentrations, measured by specific radiommunoassay from 13 ± 5 pg/ml in control rats to 107 + 9 pg/ml in the rats killed 10 min after injection, a similar response to that observed previously in urethane-anaesthetized rats.5. In each of six urethane-anaesthetized rats, recordings were made from single neurones in the supraoptic nucleus, identified antidronomically as projecting to the posterior pituitary and identified electrophysiologically as putative oxytocin neurones. Following i.P. injection of 50 jug/kg CCK, the neurones increased their firing rate by a mean of 1-3 +0-2 spikes/s averaged over the O min following injection.6. From the appearance of c-fos mRNA in supraoptic neurones following CCK administration we conclude that this message is expressed in magnocellular oxytocin neurones, since vasopressin neuronal activity and vasopressin release is known to be t Permanent address:

show abstract

Section: C-fos Mrna In Oxytocin Neurones Discussionmentioning

confidence: 99%

Electrical activation and c‐fos mRNA expression in rat neurosecretory neurones after systemic administration of cholecystokinin.

Hamamura

Leng

Emson

et al. 1991

The Journal of Physiology

View full text Add to dashboard Cite

show abstract

“…The block was immersed in the same fixative as used for the perfusion for 24 h at 4 C, and then in 0.1 M PB containing 20% sucrose at 4 C until it sank. Forty µm coronal sections were cut serially on the freezing microtome, and were kept in the cryoprotectant solution [37] at 20 C until they were processed for histochemistry.…”

Section: Methodsmentioning

confidence: 99%

Nitric Oxide in the Ventromedial Nucleus of the Hypothalamus Mediates Action of Estrogen on Goat Ingestive Behavior.

Aoyama

Takeuchi

Mori

et al. 1998

Journal of Reproduction and Development

View full text Add to dashboard Cite

Abstract. To examine the hypothesis that whether nitric oxide (NO) in the brain is involved in actions of estrogen on reproduction and ingestion in ruminant species, three experiments were conducted using ovariectomized (ovx) or ovx plus estradiol (E) treated goats. In experiment 1, distributions of cells containing estrogen receptor (ER) or NO synthase (NOS) in the hypothalamus were determined immunohistochemically, which revealed that both ER and NOS positive cells were localized abundantly in the ventromedial nucleus of the hypothalamus (VMH). In experiment 2, several doses of sodium nitroprusside (SNP), an NO donor, was administered into the unilateral VMH of ovx goats immediately before the presentation of food. Injection of 1.5 µg SNP resulted in an abrupt decrease in food intake during the first 60-min of a 240-min feeding period. However, higher doses of SNP had no effect on food intake. In experiment 3, on the other hand, N Gmonomethyl-L-arginine (L-NMMA), a NOS inhibitor, was injected into the bilateral VMH of animals which had been treated with E. Although following the vehicle injection food intake was significantly suppressed by E as was seen in the VMH intact animal, this E-induced decrease in food intake was prevented by administering L-NMMA. This NOS inhibitor, on the other hand, altered neither the timing of the luteinizing hormone surge nor estrous behavior. These data suggest that NO in the VMH is involved in mediation of suppressive effect of E on ingestive behavior in the goat. Key words: Ingestive behavior, Estrogen, Nitric oxide (NO), Ventromedial nucleus of the hypothalamus (VMH), Goat.(J. Reprod. Dev. 44: [149][150][151][152][153][154][155][156][157][158][159] 1998) e have shown that administration of physiological levels of estrogen induces reduction fied, the ventromedial nucleus of the hypothalamus (VMH) seems to be one of the most plausible targets for estrogen to simultaneously alter reproductive and ingestive functions. The VMH is thought to be an important center for controlling reproductive [1] as well as ingestive [2] behaviors, and it has been demonstrated in a number of species [3][4][5][6] that this nucleus contains estrogen receptor (ER) in abundant. Previous observations that direct application of estrogen to the VMH of food intake and rumination at the time of estrus behavior manifestation and the luteinizing hormone (LH) surge in ovariectomized (ovx) goats [Aoyama et al., submitted]. Although sites of these estrogen actions in the brain are yet to be identi-W

show abstract

“…The animals were deeply anesthetized with 4% chloral hydrate (3-5 ml, intraperitoneally) and perfused transcardially (4% paraformaldehyde + 5% acrolein) as previously described by Hoffman et al 20 The fixed brains were removed and placed in a 25% sucrose solution for 2 days before sectioning. The brains were then cut frozen in the coronal plane at a thickness of 30 m, and serial sections were stored in cryoprotectant solution (500 ml sodium phosphate buffer, 300 g sucrose, 10 g polyvinylpyrrolidone, and 300 ml ethylene glycol; final volume 1000 ml; 0.1 M, pH 7.2).…”

Section: Immunohistochemical Analysismentioning

confidence: 99%

“…Sections as far rostral as the OVLT (0.5 mm anterior to 20 The primary antiserum LR-1 (provided by Dr. Robert Benoit) recognizes both precursor and decapeptide forms of GnRH. Briefly, the brain tissue sections were removed from the cryoprotectant, rinsed in PBS, treated with 1% NaBH 4 solution (Sigma), rinsed, and incubated with the anti-GnRH antibody at a concentration of 1:100,000 in PBS with 0.4% Triton X-100 for 48 hours at 4˚C.…”

Section: Immunohistochemical Analysismentioning

confidence: 99%

See 1 more Smart Citation

Effects of congenital hydrocephalus on the hypothalamic gonadotrophin-releasing hormone system

McAllister

Abdolvahabi

Walker

et al. 2007

FOC

View full text Add to dashboard Cite

Object Despite the investigations that have linked hydrocephalus to reproductive system abnormalities, no researchers have attempted to identify the pathophysiological mechanism of this relationship. Because the role of the hypothalamic gonadotrophin-releasing hormone (GnRH) system in the regulation of reproductive functions is well established, the authors used immunohistochemical and radioimmunoassay (RIA) techniques to determine the morphological and biochemical effects of hydrocephalus on the hypothalamic GnRH system. Methods Hypothalamic GnRH levels, fiber density, and cell types were studied in 21- and 50-day-old control and congenitally hydrocephalic Texas rats. Results of RIA indicated a significant (8.4%) increase in GnRH in 21-day-old hydrocephalic rats (9.17 ± 0.64 pg/ng total protein) compared with that in controls (0.97 ± 0.74 pg/ng total protein). In addition, the 50-day-old hydrocephalic animals had a significantly higher level of GnRH compared with age-matched controls (20.4 pg/ng compared with 1.88 ± 2.1 pg/ng total protein). This increase was accompanied by changes in the fiber appearance and a shift from low GnRH producing cells to high GnRH producing cells in the hydrocephalic animals; however, there was no significant difference in the fiber density between the control and hydrocephalic animals at 21 days. In addition, poor neurological scores correlated with the severity of hydrocephalus. Conclusions These results demonstrated that hypothalamic GnRH levels are significantly affected by fetal-onset hydrocephalus and that the mechanisms responsible for these effects may take place at the cellular rather than the gross structural level. Furthermore, they suggest that impairments in the GnRH system may be protracted in neonates and infants with hydrocephalus, and thus may be overcome by relatively early treatment with ventricular diversion. However, the clinical implications of GnRH perturbations in shunt-dependent patients must await a forthcoming study in shunted animals.

show abstract

Luteinizing Hormone-Releasing Hormone Neurons Express c-fosAntigen after Steroid Activation*

Cited by 199 publications

References 11 publications

Electrical activation and c‐fos mRNA expression in rat neurosecretory neurones after systemic administration of cholecystokinin.

Electrical activation and c‐fos mRNA expression in rat neurosecretory neurones after systemic administration of cholecystokinin.

Nitric Oxide in the Ventromedial Nucleus of the Hypothalamus Mediates Action of Estrogen on Goat Ingestive Behavior.

Effects of congenital hydrocephalus on the hypothalamic gonadotrophin-releasing hormone system

Contact Info

Product

Resources

About