Luteolin decreases the attachment, invasion and cytotoxicity of UPEC in bladder epithelial cells and inhibits UPEC biofilm formation

Shen, Xiaofei; Ren, L.; Teng, Yan; Zheng, Shuang; Yang, Xiao‐Long; Guo, Xingyi; Wang, Xinyuan; Sha, Kaihui; Li, Na; Xu, Guangya; Tian, Hongqi; Wang, Xiaoying; Liu, Xiao-Kang; Li, Jingyu; Huang, Ning

doi:10.1016/j.fct.2014.07.019

Cited by 39 publications

(26 citation statements)

References 40 publications

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“…Therefore, it is important to carry out studies on novel strategies against bacterial biofilm. Natural products or food-derived agents are attracting great attention for their anti-biofilm abilities [ 20 , 21 ]. Garlic-derived compounds, such as ajoene and allicin, have shown great antibacterial potential.…”

Section: Discussionmentioning

confidence: 99%

“…Type 1 pili adhesin FimH recognizes the glycoprotein uroplakin Ia/IIIa and α3/β1 integrin of bladder epithelial cells and thereby mediates UPEC attachment and invasion [ 15 ]. Neutralization of FimH or disruption of its encoding gene impaired the ability of UPEC to colonize the bladder epithelium [ 29 ] and the agent downregulated fimH gene showed an inhibitory effect on UPEC attachment and invasion in T24 cells [ 20 ]. RT-qPCR study showed that the expression level of fimH was significantly decreased in the presence of 50 µg/mL allicin, indicating the inhibitory effect of allicin on UPEC attachment in T24 cells was probably due to its ability to decrease the expression of fimH gene.…”

Section: Discussionmentioning

confidence: 99%

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Subinhibitory Concentrations of Allicin Decrease Uropathogenic Escherichia coli (UPEC) Biofilm Formation, Adhesion Ability, and Swimming Motility

Yang

Sha

et al. 2016

IJMS

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Uropathogenic Escherichia coli (UPEC) biofilm formation enables the organism to avoid the host immune system, resist antibiotics, and provide a reservoir for persistent infection. Once the biofilm is established, eradication of the infection becomes difficult. Therefore, strategies against UPEC biofilm are urgently required. In this study, we investigated the effect of allicin, isolated from garlic essential oil, on UPEC CFT073 and J96 biofilm formation and dispersal, along with its effect on UPEC adhesion ability and swimming motility. Sub-inhibitory concentrations (sub-MICs) of allicin decreased UPEC biofilm formation and affected its architecture. Allicin was also capable of dispersing biofilm. Furthermore, allicin decreased the bacterial adhesion ability and swimming motility, which are important for biofilm formation. Real-time quantitative polymerase chain reaction (RT-qPCR) revealed that allicin decreased the expression of UPEC type 1 fimbriae adhesin gene fimH. Docking studies suggested that allicin was located within the binding pocket of heptyl α-d-mannopyrannoside in FimH and formed hydrogen bonds with Phe1 and Asn135. In addition, allicin decreased the expression of the two-component regulatory systems (TCSs) cognate response regulator gene uvrY and increased the expression of the RNA binding global regulatory protein gene csrA of UPEC CFT073, which is associated with UPEC biofilm. The findings suggest that sub-MICs of allicin are capable of affecting UPEC biofilm formation and dispersal, and decreasing UPEC adhesion ability and swimming motility.

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Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Subinhibitory Concentrations of Allicin Decrease Uropathogenic Escherichia coli (UPEC) Biofilm Formation, Adhesion Ability, and Swimming Motility

Yang

Sha

et al. 2016

IJMS

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“…Luteolin, by interfering with PDE activity, inhibits lymphocyte function-associated antigen-1 (LFA-1) expression and neutrophil adhesion to endothelial cells, thus acting as an antinflammatory agent (Jiang et al, 2015 ) and a protectant of bladder epithelial cells against UPEC (Shen et al, 2016 ). Furthermore, this flavone inhibits UPEC-induced cytotoxicity and decreases both attachment and biofilm formation in pre-treated UPEC (Shen et al, 2014 ). Bacterial fatty acid synthesis, in particular β-ketoacyl acyl carrier protein synthase (KAS) enzymes, are good therapeutic targets for novel antibiotics.…”

Section: Alternative Antimicrobial Remediesmentioning

confidence: 99%

UroPathogenic Escherichia coli (UPEC) Infections: Virulence Factors, Bladder Responses, Antibiotic, and Non-antibiotic Antimicrobial Strategies

2017

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Urinary tract infections (UTIs) are one of the most common pathological conditions in both community and hospital settings. It has been estimated that about 150 million people worldwide develop UTI each year, with high social costs in terms of hospitalizations and medical expenses. Among the common uropathogens associated to UTIs development, UroPathogenic Escherichia coli (UPEC) is the primary cause. UPEC strains possess a plethora of both structural (as fimbriae, pili, curli, flagella) and secreted (toxins, iron-acquisition systems) virulence factors that contribute to their capacity to cause disease, although the ability to adhere to host epithelial cells in the urinary tract represents the most important determinant of pathogenicity. On the opposite side, the bladder epithelium shows a multifaceted array of host defenses including the urine flow and the secretion of antimicrobial substances, which represent useful tools to counteract bacterial infections. The fascinating and intricate dynamics between these players determine a complex interaction system that needs to be revealed. This review will focus on the most relevant components of UPEC arsenal of pathogenicity together with the major host responses to infection, the current approved treatment and the emergence of resistant UPEC strains, the vaccine strategies, the natural antimicrobial compounds along with innovative anti-adhesive and prophylactic approaches to prevent UTIs.

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“…Moreover, their efficacy towards mature biofilms of Borrelia sp. is encouraging and showed to have a possible wilder bacterial spectrum (Cao et al 2008;Shen et al 2014;Chen et al 2016). Furthermore, our results showed that the DNA of studied Borrelia sp.…”

Section: Discussionmentioning

confidence: 93%

Reciprocal cooperation of phytochemicals and micronutrients against typical and atypical forms ofBorreliasp.

2017

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Luteolin decreases the attachment, invasion and cytotoxicity of UPEC in bladder epithelial cells and inhibits UPEC biofilm formation

Cited by 39 publications

References 40 publications

Subinhibitory Concentrations of Allicin Decrease Uropathogenic Escherichia coli (UPEC) Biofilm Formation, Adhesion Ability, and Swimming Motility

Subinhibitory Concentrations of Allicin Decrease Uropathogenic Escherichia coli (UPEC) Biofilm Formation, Adhesion Ability, and Swimming Motility

UroPathogenic Escherichia coli (UPEC) Infections: Virulence Factors, Bladder Responses, Antibiotic, and Non-antibiotic Antimicrobial Strategies

Reciprocal cooperation of phytochemicals and micronutrients against typical and atypical forms ofBorreliasp.

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