Luteolin restricts ASFV replication by regulating the NF-κB/STAT3/ATF6 signaling pathway

Yang, Chen; Guo, Yanchen; Song, Zebu; Chang, Hao; Kuang, Qiyuan; Zheng, Zezhong; Wang, Heng; Zhang, Guihong

doi:10.1016/j.vetmic.2022.109527

Cited by 20 publications

(11 citation statements)

References 43 publications

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“…Several methods or compounds, including nucleoside analogs, interferons, antibiotics, small interfering RNAs, CRISPR/Cas9, and plant-derived compounds, have been established with in vitro anti-ASFV activity in the past few decades [9][10][11][12][13][14][15][16][17][18][19]. Among them, natural compounds derived from plants exhibit multiple potential targets, bioactivities, and limited toxicity, which occupy more than half of the approved drugs or drug candidates in the past three decades [20,21].…”

Section: Introductionmentioning

confidence: 99%

Berbamine Hydrochloride Inhibits African Swine Fever Virus Infection In Vitro

et al. 2022

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African swine fever virus (ASFV) causes a viral disease in swine with a mortality rate of approximately 100%, threatening the global pig industry's economic development. However, vaccines are not yet commercially available, and other antiviral therapeutics, such as antiviral drugs, are urgently needed. In this study, berbamine hydrochloride, a natural bis-benzylisoquinoline alkaloid isolated from the traditional Chinese herb Berberis amurensis, showed significant antiviral activity against ASFV. The 50% cytotoxic concentration (CC50) of berbamine hydrochloride in porcine alveolar macrophages (PAMs) was 27.89 μM. The antiviral activity assay demonstrated that berbamine hydrochloride inhibits ASFV in a dose-dependent manner. In addition, a 4.14 log TCID50 decrease in the viral titre resulting from non-cytotoxic berbamine hydrochloride was found. Moreover, the antiviral activity of berbamine hydrochloride was maintained for 48h and took effect at multiplicities of infection (MOI) of 0.01, 0.1, and 1. The time-of-addition analysis revealed an inhibitory effect throughout the entire virus life-cycle. A subsequent viral entry assay verified that berbamine hydrochloride blocks the early stage of ASFV infection. Moreover, similar anti-ASFV activity of berbamine hydrochloride was also found in PK-15 and 3D4/21 cells. In summary, these results indicate that berbamine hydrochloride is an effective anti-ASFV natural product and may be considered a novel antiviral drug.

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Section: Introductionmentioning

confidence: 99%

Berbamine Hydrochloride Inhibits African Swine Fever Virus Infection In Vitro

et al. 2022

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“…It has been reported that the NF-κB signaling pathway was activated by ASFV infection, and the NF-κB signaling pathway inhibitor can inhibit ASFV replication [ 21 ]. We further explored the effect of Ae on the activation of the NF-κB signaling pathway in ASFV infection.…”

Section: Resultsmentioning

confidence: 99%

Aloe-emodin inhibits African swine fever virus replication by promoting apoptosis via regulating NF-κB signaling pathway

Luo

Yang

Wang

et al. 2023

Virol J

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African swine fever (ASF) is an acute infectious haemorrhagic fever of pigs caused by African swine fever virus (ASFV). Aloe-emodin (Ae) is an active ingredient of Chinese herbs with antiviral, anticancer, and anti-inflammatory effects. We investigated the antiviral activity and mechanism of action of Ae against ASFV using Real-time quantitative PCR (qPCR), western blotting, and indirect immunofluorescence assays. Ae significantly inhibited ASFV replication. Furthermore, transcriptomic analysis revealed that ASFV infection activated the NF-κB signaling pathway in the early stage and the apoptosis pathway in the late stage. Ae significantly downregulated the expression levels of MyD88, phosphor-NF-κB p65, and pIκB proteins as well as the mRNA levels of IL-1β and IL-8 in porcine alveolar macrophages (PAMs) infected with ASFV, thereby inhibiting the activation of the NF-κB signaling pathway induced by ASFV. Flow cytometry and western blot analysis revealed that Ae significantly increased the percentage of ASFV-induced apoptotic cells. Additionally, Ae promoted apoptosis by upregulating the expression levels of cleaved-caspase3 and Bax proteins and downregulating the expression levels of Bcl-2 proteins. This suggests that Ae promotes apoptosis by inhibiting the NF-κB pathway, resulting in inhibition of ASFV replication. These findings have further improved therapeutic reserves for the prevention and treatment of ASF.

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“…Among them, Platycodin-D has been experimentally shown to protect against acute lung injury by inhibiting apoptosis through the Bax/Bcl-2/Caspase-3 signaling pathway, and is also associated with the NF-κB pathway, which can inhibit inflammation and oxidative stress [10,11]. Luteolin is associated with the NF-κB/STAT3/ATF6 signaling pathway, which has an inhibitory effect on the inflammatory response [12]. Acacetin plays an important role as a natural flavonoid in lung injury due to sepsis [13].…”

Section: Discussionmentioning

confidence: 99%

Exploring the mechanism of action of three agents white powder in the care for pulmonary diseases based on network pharmacology and technology for molecular docking

Ai-qi

2023

Second International Conference on Biological Engineering and Medical Science (ICBioMed 2022)

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Based on the international concern about COVID-19 and pulmonary diseases, the number of cases of lung injury caused by COVID-19 pneumonia and its complications has increased dramatically in recent years, and the complexity of the situation makes the combination of single drugs a major problem in respiratory diseases. Therefore, it would be feasible to replace single drug combinations with compounded formulations for the treatment of a diverse array of pulmonary diseases. At the same time, the visualization of the chemical composition of herbal formulations and the study of molecular interactions to reveal the mechanism of action will be of practical significance. This paper introduced the therapeutic mechanisms of the traditional Chinese medicine formula Three Agents White Powder. Three major pulmonary diseases, COVID-19, pulmonary fibrosis, and lung abscess, were investigated using molecular docking and network pharmacology approaches. Mainly using TCMSP, DAVID, STRING, Genecard database screening, and autodock molecular docking methods, this paper verified that this drug applies its healing benefits to pulmonary diseases through multiple components, multiple targets, and multiple pathways, and illustrated the effectiveness of this formula in the adjuvant treatment of extensive pulmonary diseases.

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Luteolin restricts ASFV replication by regulating the NF-κB/STAT3/ATF6 signaling pathway

Cited by 20 publications

References 43 publications

Berbamine Hydrochloride Inhibits African Swine Fever Virus Infection In Vitro

Berbamine Hydrochloride Inhibits African Swine Fever Virus Infection In Vitro

Aloe-emodin inhibits African swine fever virus replication by promoting apoptosis via regulating NF-κB signaling pathway

Exploring the mechanism of action of three agents white powder in the care for pulmonary diseases based on network pharmacology and technology for molecular docking

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