Luteoloside attenuates anoxia/reoxygenation‐induced cardiomyocytes injury via mitochondrial pathway mediated by 14‐3‐3η protein

Abstract

Ischemia/reperfusion (I/R) injury is the major cause of acute cardiovascular disease worldwide. 14-3-3η protein has been demonstrated to protect myocardium against I/R injury. Luteoloside (Lut), a flavonoid found in many Chinese herbs, exerts myocardial protection effects. However, the mechanism remains unclear. We hypothesize that the cardioprotective role of Lut is exerted by regulating the 14-3-3η signal pathway. To investigate our hypothesis, an in vitro I/R model was generated in H9C2 cardiomyocytes by anoxia/reoxygenation (A/R) treatment. The effects of Lut on cardiomyocytes with A/R injury were assessed by determining the cell viability, lactate dehydrogenase levels, intracellular reactive oxygen species levels, mitochondrial permeability transition pores (mPTP) openness, caspase-3 activity, and apoptosis rate. The effects on protein expression were tested using western blot analysis. Lut attenuated A/R-induced injury to cardiomyocytes by increasing the expression of 14-3-3η protein and cell viability; decreasing levels of lactate dehydrogenase, reactive oxygen species, mPTP openness, caspase-3 activity, and low apoptosis rate were observed. However, the cardioprotective effects of Lut were blocked by AD14-3-3ηRNAi, an adenovirus knocking down the intracellular 14-3-3η expression. In conclusion, to our knowledge, this is the first study to demonstrate that Lut protected cardiomyocytes from A/R-induced injury via the regulation of 14-3-3η signaling pathway.

Capsaicin Alleviates the Deteriorative Mitochondrial Function by Upregulating 14-3-3η in Anoxic or Anoxic/Reoxygenated Cardiomyocytes

“…14-3-3 is the molecular target of many active ingredients of plants. We have confirmed that 14-3-3 assists PKCε, Bcl-2, and other functional proteins to locate to mitochondria and protect cardiomyocytes and vascular endothelial cells against multiple injuries [7,9,[40][41][42][43]. Further studies are needed to define specific mechanism(s) of action for Cap-activated 14-3-3η in anoxia and A/R injured cardiomyocytes.…”

Remedying the Mitochondria to Cure Human Diseases by Natural Products

“…Propolis [96] Luteoloside [99] Eriodictyol [101] Dihydromyricetin [102] Vitexin [103] Apigenin [105] Oxidative stress…”

“…Capsaicin [97] Quercetin [98] Luteoloside [99] Astragaloside IV [100] Eriodictyol [101] Dihydromyricetin [102] Vitexin [103] Honokiol [104] Apigenin [105] Apoptosis Capsaicin [97] Quercetin [98] Luteoloside [99] Astragaloside IV [100] Eriodictyol [101] Dihydromyricetin [102] Apigenin [105] Mitochondrial membrane potential imbalance…”

“…Capsaicin [97] Quercetin [98] Luteoloside [99] Astragaloside IV [100] Eriodictyol [101] Vitexin [103] Honokiol [104] Apigenin [105] Nature products…”

Galuteolin suppresses proliferation and inflammation in TNF-α-induced RA-FLS cells by activating HMOX1 to regulate IKKβ/NF-κB pathway

“…Galuteolin exhibited a high activity against in uenza virus (H3N2) [9]. Galuteolin could alleviate myocardial cell damage caused by myocardial ischemia-reperfusion by reducing oxidative stress response and inhibiting myocardial cell apoptosis [10]. Galuteolin blocked the activation of NF-κB and TLR2 signaling pathways, thereby reducing the in ammation, damage and apoptosis of uterine cells caused by staphylococcus aureus [11].…”