Lutispora thermophila gen. nov., sp. nov., a thermophilic, spore-forming bacterium isolated from a thermophilic methanogenic bioreactor digesting municipal solid wastes

Abstract

Lutispora thermophila gen. nov., sp. nov., a thermophilic, spore-forming bacterium isolated from a thermophilic methanogenic bioreactor digesting municipal solid wastes The genus Clostridium, comprising Gram-positive species, was first proposed by Prazmowski in 1880. At the time of writing, the names of 188 species of the genus have been validly published (http://www.bacterio.cict.fr). Most members of the genus are chemo-organotrophs that can utilize carbohydrates and/or proteinaceous compounds as an energy source. In classical taxonomy, an organism only had to meet four criteria to be classified as a member of the genus Clostridium, i.e. be endospore-forming, be obligately anaerobic, have a Gram-positive cell wall and lack the ability to carry out dissimilatory reduction of sulfate. The genus was thus highly divergent and included psychrophilic, mesophilic and thermophilic species with a wide range of DNA G+C content (21-54 mol%).To clarify the phylogenetic incoherence within the genus Clostridium, Collins et al. In the present study, we investigated the taxonomy of a novel member of the family Clostridiaceae (strain EBR46 T ), isolated from thermophilic methanogenic sludge. In a previous study, we successfully determined the 16S rRNA gene sequence of this organism but failed to isolate it owing, to a large part, to its inefficient recovery from long-term storage at 280 u C (Shiratori et al., 2006). Following longterm successive cultivations, we succeeded in isolating and stably culturing strain EBR46 T . The distinct phylogenetic lineage represented by this isolate fell within a position close to members of the genus Gracilibacter outside the cluster of members of Clostridium sensu stricto. Both phylogenetic and Abbreviation: DMA, dimethyl acetal.

Cellulose- and Xylan-Degrading Thermophilic Anaerobic Bacteria from Biocompost

“…It is known that C. thermosuccinogenes is able to use glucose, xylose, and cellobiose (7). On the other hand, L. thermophila did not utilize any of the detected Avicel fermentation products or intermediates but was able to degrade cysteine (31). Thus, it is likely that strain 6-12, related to C. thermosuccinogenes, survived in consortia by utilizing cellobiose and glucose and that strain 6-30, related to L. thermophila, had been enriched on L-cysteine.…”

“…The first group of moderately thermophilic bacteria contained four isolates that were not able to utilize cellulose. These isolates were related to different taxonomic groups, including noncellulolytic clostridia (C. thermosuccinogenes), the recently described anaerobic bacterium Lutispora thermophila (31), and previously uncultured microorganisms from cellulose-degrading methanogenic reactors (5,38). All of these isolates were able to grow as pure cultures on cellobiose medium with L-cysteine as a reducing agent, and they sustained ϳ10 transfers within cellulolytic consortia on defined cellulose-mineral medium.…”

Diversity of Bacteria and Glycosyl Hydrolase Family 48 Genes in Cellulolytic Consortia Enriched from Thermophilic Biocompost

“…Two OTUs from the CO-6 enrichment, representing 1% to 3% of this clone library each, were determined to be most similar to the newly isolated Lutispora thermophila, which is a noncellulolytic organism from a thermophilic methanogenic reactor (29). Likewise, a minor component of the CO-5 enrichment was most closely related to a proposed novel clostridium within cluster XIV, Clostridium islandicum (GenBank accession no.…”

“…All OTUs from the CO-4 enrichment grouped within cluster III, as did 7 out of 8 OTUs from the CO-5 enrichment. However, not only did the CO-6 enrichment have the largest number of detected OTUs, but they were spread across clusters I, III, and XIV and a novel cluster of which the novel clostridium Lutispora thermophila (29) is the main representative. Clostridium straminisolvens-like sequences were consistently found in all three enrichments, although in different proportions.…”

Amide-transforming activity of Streptomyces: possible application to the formation of hydroxy amides and aminoalcohols

“…The product structure was confirmed by NMR. Phylogenetic analysis PCR amplification was used to generate most of the 16S rRNA gene sequence for each isolate by using the B8F/B1492R primers (Shiratori et al 2008) and then sequenced by an ABI 3130 genetic analyzer (Applied Biosystems, Tokyo), according to the manufacturer's protocol. Around 1,383 bp of the 16S rRNA gene sequence of SY007 and SY435 were compared with those from the GenBank/EMBL/DDBJ nucleotide sequence database (http://www.ncbi.nlm.nih.gov/BLAST/).…”