LUTTE CHIMIQUE CONTRE LA HERNIE, <i>PLASMODIOPHORA BRASSICAE</i> WOR., DES CRUCIFERES EN SOLS MINERAL ET ORGANIQUE

Crête, R.; Laliberte, Jean Francois; Jasmin, J. J.

doi:10.4141/cjps63-065

Cited by 21 publications

(11 citation statements)

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“…Roots of 7-14 plants per line were assessed for clubroot severity at 6 weeks after inoculation using a standard 0 to 3 scale where: 0 = no clubbing; 1 = small clubs only; 2 = moderate clubs; and 3 = severe clubbing. A disease severity index (DSI) [ 63 , 64 ] was calculated using the following formula: …”

Section: Methodsmentioning

confidence: 99%

Identification of Genome-Wide Variants and Discovery of Variants Associated with Brassica rapa Clubroot Resistance Gene Rcr1 through Bulked Segregant RNA Sequencing

et al. 2016

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Clubroot, caused by Plasmodiophora brassicae, is an important disease on Brassica species worldwide. A clubroot resistance gene, Rcr1, with efficacy against pathotype 3 of P. brassicae, was previously mapped to chromosome A03 of B. rapa in pak choy cultivar “Flower Nabana”. In the current study, resistance to pathotypes 2, 5 and 6 was shown to be associated with Rcr1 region on chromosome A03. Bulked segregant RNA sequencing was performed and short read sequences were assembled into 10 chromosomes of the B. rapa reference genome v1.5. For the resistant (R) bulks, a total of 351.8 million (M) sequences, 30,836.5 million bases (Mb) in length, produced 120-fold coverage of the reference genome. For the susceptible (S) bulks, 322.9 M sequences, 28,216.6 Mb in length, produced 109-fold coverage. In total, 776.2 K single nucleotide polymorphisms (SNPs) and 122.2 K insertion / deletion (InDels) in R bulks and 762.8 K SNPs and 118.7 K InDels in S bulks were identified; each chromosome had about 87% SNPs and 13% InDels, with 78% monomorphic and 22% polymorphic variants between the R and S bulks. Polymorphic variants on each chromosome were usually below 23%, but made up 34% of the variants on chromosome A03. There were 35 genes annotated in the Rcr1 target region and variants were identified in 21 genes. The numbers of poly variants differed significantly among the genes. Four out of them encode Toll-Interleukin-1 receptor / nucleotide-binding site / leucine-rich-repeat proteins; Bra019409 and Bra019410 harbored the higher numbers of polymorphic variants, which indicates that they are more likely candidates of Rcr1. Fourteen SNP markers in the target region were genotyped using the Kompetitive Allele Specific PCR method and were confirmed to associate with Rcr1. Selected SNP markers were analyzed with 26 recombinants obtained from a segregating population consisting of 1587 plants, indicating that they were completely linked to Rcr1. Nine SNP markers were used for marker-assisted introgression of Rcr1 into B. napus canola from B. rapa, with 100% accuracy in this study.

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Section: Methodsmentioning

confidence: 99%

Identification of Genome-Wide Variants and Discovery of Variants Associated with Brassica rapa Clubroot Resistance Gene Rcr1 through Bulked Segregant RNA Sequencing

et al. 2016

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“…A disease index (DI) was calculated using the formula; where P\, P2 and P^ are the percentages of plants in categories 1, 2 and 3 respectively. This formula is based on that used by Crete et al {1963). Trials in 1979 were scored into five categories (Dixon & Doodson, 1970, 1971) from which two DIs were calculated;…”

Section: Methodsmentioning

confidence: 99%

The susceptibility of Brassica oleracea cultivars to Plasmodiophora brassicae (clubroot)

Dixon¹,

Robinson

1986

Plant Pathology

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An evaluation of 404 Brassica oleracea cultivars for susceptibility to Plasmodiophora brassicae (clubroot) at one field site in northern Scotland over the four years 1979‐82 is reported. The population of P. brassicae at this site possessed a high degree of virulence to B. oleracea as determined by seedling tests in a glasshouse environment, using the European Clubroot Differential series. No statistically significant differences in susceptibility were consistently identified between cultivars when tested under field conditions. Data over years and over crops were analysed by the residual maximum likelihood technique. Differences between cultivars were small compared with cultivar x year interactions. No obvious differences were identified in susceptibility between the crop types Brussels sprout, cabbage, calabrese and cauliflower. Disease levels as analysed over all field trials increased markedly in years three and four of this experiment compared with the first two years.

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“…Disease reaction was determined six weeks after the inoculation. Disease rating was based on that proposed for Plasmodiophora brassicae by CRETE et al (1963) namely, 0, no infection; 1, 1 to 29 '%; 2, 30 to 59 o/o and 3, 60-100 '/& root infection.…”

Section: Introductionmentioning

confidence: 99%

Transfer of resistance to race 2 of Plasmodiophora brassicae from Brassica napus to cabbage (B. Oleracea spp. Capitata). IV. A resistant 18-chromosome B1 plant and its B2 progenies

Chiang

Grant

et al. 1980

Euphytica

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LUTTE CHIMIQUE CONTRE LA HERNIE, PLASMODIOPHORA BRASSICAE WOR., DES CRUCIFERES EN SOLS MINERAL ET ORGANIQUE

Cited by 21 publications

References 0 publications

Identification of Genome-Wide Variants and Discovery of Variants Associated with Brassica rapa Clubroot Resistance Gene Rcr1 through Bulked Segregant RNA Sequencing

Identification of Genome-Wide Variants and Discovery of Variants Associated with Brassica rapa Clubroot Resistance Gene Rcr1 through Bulked Segregant RNA Sequencing

The susceptibility of Brassica oleracea cultivars to Plasmodiophora brassicae (clubroot)

Transfer of resistance to race 2 of Plasmodiophora brassicae from Brassica napus to cabbage (B. Oleracea spp. Capitata). IV. A resistant 18-chromosome B1 plant and its B2 progenies

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