Ly49 and CD94/NKG2: developmentally regulated expression and evolution

Takei, Fumio; McQueen, Karina L.; Maeda, Motoi; Wilhelm, Brian T.; Lohwasser, Stefan; Lian, Rebecca H.; Mager, Dixie L.

doi:10.1034/j.1600-065x.2001.1810107.x

Cited by 59 publications

(55 citation statements)

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“…It is well established that some Ly49 family members having an ITIM associate with Src homology 2 domain-containing tyrosine phosphatases such as SHP-1 and SHP-2 (7,8). To examine whether Ly49Q could engage phosphatases in the cytoplasmic ITIM, COS7 cells were transfected with either FLAG-tagged Ly49Q WT cDNA or Ly49Q YF mutant cDNA in which the tyrosine residue in the ITIM was substituted to phenylalanine.…”

Section: Resultsmentioning

confidence: 99%

“…It has been postulated that recognition of MHC class I, or related molecules, on target cells by inhibitory NK receptors allows NK cells to prevent self-killing but destroy inappropriate cells possessing decreased levels of MHC class I (4-6). In mice, two types of inhibitory receptors on NK cells have been identified that belong to the Ly49 family and CD94͞NKG2 family (7)(8)(9).…”

mentioning

confidence: 99%

“…Ly49 family members are expressed on subsets of NK and NKT cells as disulfide-linked dimers (7)(8)(9). The Ly49 family belongs to a group of type II integral membrane protein that contains external domains homologous to the superfamily of Ca 2ϩ -dependent lectins (10).…”

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confidence: 99%

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Ly49Q, a member of the Ly49 family that is selectively expressed on myeloid lineage cells and involved in regulation of cytoskeletal architecture

Toyama–Sorimachi

Tsujimura

Maruya

et al. 2004

Proc. Natl. Acad. Sci. U.S.A.

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Here, we identified and characterized a Ly49 family member, designated as Ly49Q. The Ly49q gene encodes a 273-aa protein with an immunoreceptor tyrosine-based inhibitory motif (ITIM) at the N terminus of its cytoplasmic domain. We show that the ITIM of Ly49Q can recruit SHP-2 and SHP-1 in a tyrosine phosphorylation-dependent manner. In contrast to other known members of the Ly49 family, Ly49Q was found not to be expressed on NK1.

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Section: Resultsmentioning

confidence: 99%

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confidence: 99%

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Ly49Q, a member of the Ly49 family that is selectively expressed on myeloid lineage cells and involved in regulation of cytoskeletal architecture

Toyama–Sorimachi

Tsujimura

Maruya

et al. 2004

Proc. Natl. Acad. Sci. U.S.A.

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“…3 Recent reports suggest that the self-recognizing NK-cell receptor repertoire is built in successive fashion following contact of NK-cell precursors with environmental class I MHC molecules during development. 3,4 However, many questions remain about the origin and lifespans of NK cells. Although still incomplete, a differentiation sequence is emerging that will eventually describe how hematopoietic stem cells generate NK cells in mice and humans.…”

Section: Introductionmentioning

confidence: 99%

“…Some additional NK molecules have been suggested to function as activating receptors. 3 Recent reports suggest that the self-recognizing NK-cell receptor repertoire is built in successive fashion following contact of NK-cell precursors with environmental class I MHC molecules during development. 3,4 However, many questions remain about the origin and lifespans of NK cells.…”

Section: Introductionmentioning

confidence: 99%

Relationships between early B- and NK-lineage lymphocyte precursors in bone marrow

Kouro

Kumar

Kincade

2002

Blood

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IntroductionNatural killer (NK) cells have long been recognized for their importance in innate immunity, viral defense, and tumor surveillance. 1 Additionally, NK cells represent barriers to bone marrow transplantation and produce cytokines that influence other cellular components of the immune system. Impressive progress has recently been made in understanding how NK cells recognize targets for cytolytic destruction while sparing normal cells. 2 For example, it is now clear that self-tolerance of NK cells is maintained by inhibitory receptors, which are expressed on NK cells and recognize major histocompatibility complex (MHC) class I molecules. In mice, the Ly49 receptor family recognizes classical MHC I molecules, whereas the CD94/NKG2 receptors recognize the nonclassical Qa-1 b MHC I molecule. Some additional NK molecules have been suggested to function as activating receptors. 3 Recent reports suggest that the self-recognizing NK-cell receptor repertoire is built in successive fashion following contact of NK-cell precursors with environmental class I MHC molecules during development. 3,4 However, many questions remain about the origin and lifespans of NK cells. Although still incomplete, a differentiation sequence is emerging that will eventually describe how hematopoietic stem cells generate NK cells in mice and humans. 5 Although cells with NK-lineage potential can be found among undifferentiated thymocytes, and some properties are shared with T cells, the thymus is probably not the most important site for production of NK cells. 6,7 The present study was designed to obtain more precise information about relationships between early NK-lineage precursors and those of other lymphoid lineages within bone marrow.The NK-1.1 antigen encoded by the NKR P1C gene provides a useful NK-cell marker in selected strains of mice, but is also expressed on a subset of T cells (NK T cells). The DX5 antigen, recently shown to be identical to CD49, is somewhat less restricted to NK-lineage cells, 8 and is probably acquired at a later stage. For example, there are NK-1.1 ϩ DX5 Ϫ cells in bone marrow, whereas most NK-1.1 ϩ spleen cells are DX5 ϩ . 9 It is interesting that fetal and neonatal NK cells express high levels of CD94/NKG2A receptors, but not Ly49. The density of CD94/NKG2A gradually declines when Ly49 family receptors are acquired. 10 Although interleukin 2 (IL-2) can be used to drive formation of NK cells in vitro, IL-2-deficient mice and IL-2 receptor ␣ chain (IL-2R␣)-deficient mice have NK cells. 11,12 In contrast, the IL-2R␤, the common ␥ chain (␥c) of the IL-2/IL-7 receptor and the tyrosine kinase JAK3 are all required for generation of NK cells. [13][14][15] First cloned from a human bone marrow stromal cell line, IL-15 uses a receptor composed of an IL-15-specific ␣ chain, the IL-2R␤, and the ␥c. for this cytokine were recently determined to be committed NK-cell progenitors. 9 Similar CD122 ϩ NK precursors can also be derived from multipotent Lin Ϫ Sca-2 ϩ c-kit ϩ progenitors in vitro. 23 Kondo and colleagues ...

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