Lycii fructus extract ameliorates hydrogen peroxide-induced cytotoxicity through indirect antioxidant action

Xu, Wensi; Saiki, Shunya; Myojin, Takumi; Liu, Yujia; Zhu, Beiwei; Murata, Yoshiyuki; Ashida, Hitoshi; Tsunenaga, Makoto; Nakamura, Yoshimasa

doi:10.1080/09168451.2018.1487274

Cited by 12 publications

(7 citation statements)

References 21 publications

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“…Other studies suggested that LFE ingestion was associated with increased superoxide dismutase (SOD) (56), and that goji extract administration reduced 8-OHdG levels in old mouse cavernosal tissue (57). More recently, our group demonstrated that LFE significantly enhanced the intracellular GSH levels in hepatocytes (30). However, as shown in Table V, LFE ingestion did not lead to increased antioxidative capacity in the blood as measured by total in-serum GSH levels.…”

Section: Lfe N=19mentioning

confidence: 65%

“…4 shows the effect of LFE upon intracellular total GSH, which was determined using the DTNB method. Quercetin increased total GSH at concentrations of 2.5 and 10 µM, LFE and chlorogenic acid showed no significant effect for increasing total GSH in HaCaT cells, even though LFE has been reported to enhance the cellular GSH level in hepatocytes (30).…”

Section: Protection Against Uv-induced Oxidative Stress In Hacat Cellsmentioning

confidence: 79%

“…Considering previous reports, 44 participants were recruited and 22 participants were randomized to each treatment (17,(21)(22)(23)(24). We also investigated the possibility that LFE exerts antioxidative effects in vivo as LFE has been reported to facilitate cytoprotective gene expression and enhance the production of glutathione in hepatocytes (30). To elucidate which polyphenols participate in protection against UVB-induced skin damage, we examined the effects of the LFE components on antioxidative capacity after human keratinocytes were directly exposed to UV.…”

Section: Introductionmentioning

confidence: 99%

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Modulating effects of oral administration of Lycii Fructus extracts on UVB‑induced skin erythema: A Randomized, placebo‑controlled study

Tsunenaga

Myojin

et al. 2022

Biomed Rep

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Severe UV exposure induces skin inflammation, causing erythema. Lycii Fructus (Lycium barbarum and Lycium chinense) is a potential antioxidant agent with a high content of polyphenols, including rutin and chlorogenic acid. This study examined the effects of Lycii Fructus extract (LFE) on UVB-induced skin erythema in humans. Healthy volunteers were randomly assigned to one of two groups and received UVB irradiation at 1.5 minimal erythemal dose (MED) on day 0 at three designated sites on their backs, and the skin color was measured until day 7. After an 8-week treatment with LFE (900 mg/day) or placebo, UVB irradiation (l.5 MED) was applied again at different sites on day 63. Skin color was continuously measured in each group until day 69. LFE tablet administration for 8 weeks significantly inhibited UVB-induced erythema formation and increased the MED by 13%. Erythema formation peaked on the first day after UVB irradiation, but gradually dissipated over the next several days. LFE tended to accelerate erythema disappearance. To determine the polyphenol responsible for the protection against UVB-induced skin damage, the effects of LFE-derived polyphenols and their metabolites on UVB-induced cytotoxicity were examined in vitro. The major intestinal metabolite of rutin and LFE significantly attenuated phototoxicity and in human keratinocyte HaCaT cells. Quercetin enhanced intracellular glutathione levels in HaCaT cells, even though LFE did not increase it. Together, the results showed that LFE inhibited erythema formation and accelerated erythema dissipation, possibly through its direct antioxidative action.

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Section: Lfe N=19mentioning

confidence: 65%

Section: Protection Against Uv-induced Oxidative Stress In Hacat Cellsmentioning

confidence: 79%

Section: Introductionmentioning

confidence: 99%

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Modulating effects of oral administration of Lycii Fructus extracts on UVB‑induced skin erythema: A Randomized, placebo‑controlled study

Tsunenaga

Myojin

et al. 2022

Biomed Rep

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“…41 Lycii fructus exhibits a cytoprotective effect, possibly through the enhancement of the antioxidant gene expression. 42 …”

Section: Discussionmentioning

confidence: 99%

Chinese herbal medicine for myasthenia gravis: A systematic review and meta-analysis of randomized clinical trials

Zhan¹,

Ai²,

Zhan³

et al. 2022

Integrative Medicine Research

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Background Myasthenia Gravis (MG) is a disorder of neuromuscular transmission bringing mild ocular weakness to severe generalized muscle weakness and disability. The conventional treatments have long-term side effects, and Chinese herbal medicines (CHM) have shown possible effect and safety for MG patients, but the existing evidence was not robust enough and the results were out of date. Methods Searching for randomized controlled trials (RCTs) was conducted in 7 databases and clinical trial registries until July 2021. The ROB 2 tool was used to assess the study quality and GRADE was used to assess the quality of whole evidence. Meta-analyses were conducted and the results were presented as risk ratio (RR) or mean difference (MD) with 95% confidence interval (CI). Results Nineteen RCTs (1283 participants) testing 13 kinds of CHM with adequate randomization were included and six RCTs investigating Compound Huangqi were included in the meta-analyses. In addition to conventional treatment, nine CHMs reduced symptom scores of MG. Compound Huangqi plus conventional treatment (pyridostigmine bromide or prednisone or both) reduced the symptom scores compared with conventional treatment (MD = -3.56, 95%CI -4.86 to -2.26). Less adverse events happened in the CHM groups (3/247 in the CHM groups, 52/245 in the control groups, RR = 0.13, 95%CI 0.06 to 0.30, 9 RCTs, a total of 492 participants). The effect on quality of life was inconsistent. Conclusion Nine CHMs could probably bring benefit for MG symptom improvement. Moderate to low certainty of evidence supported Compound Huangqi added-on conventional treatment probably bring extra benefit of improving MG symptoms. Adding CHMs could be safer than giving only conventional treatment. Study registration The protocol was registered in PROSPERO (ID: 32718).

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“…At present, chemical control is the main way to control pests and diseases in the production of Lycii Fructus [3]. While due to the farmers' unregulated use of pesticide, it brings hidden dangers to the quality and safety of Lycii Fructus [4,5]. Although the government enacted of regulations in 2014 stipulating 33 banned and prohibited pesticides and 17 pesticides restricted to plant crops, incidents of non-standard use of pesticides by farmers or recessive addition of prohibited ingredients to pesticides still occur from time to time.…”

Section: Introductionmentioning

confidence: 99%

A Rapid Determination of 30 Prohibited Pesticide Residues in Lycii Fructus by UPLC-MS/MS

Lin¹,

Wang²,

Wang³

et al. 2023

Phyton

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Prohibited pesticide residues have become one of the main factors affecting the quality and safety of Lycii Fructus, However, rarely studies focus on the rapid determination of these residues. Here, a total of 30 kinds of prohibited pesticide residues were determined by ultra-performance liquid chromatography tandem triple quadrupole mass spectrometry (UPLC-MS/MS) in five different process ways. Pretreatment methods, chromatographic separation and detection conditions in mass spectrometry were all optimized accordingly. Among the five different pretreatment methods, the first and third solid phase extraction failed to provide high recoveries of sulfosulfuron compounds (both lower than 60%). Recovery of chlorphenamidine by the Quick Easy Cheap Effective Rugged and Safe multiresidue method (QuEChERS) was lower than 60%, which did not meet the requirements of trace determination. The concentrations of 30 prohibited pesticides residues treated by straightforward and solid phase extraction showed good linearity in their corresponding ranges, with correlation coefficients over 0.99. The average recoveries of straightforward ranged from 78.13% to 110.9%, while RSD ranged from 1.3% to 16.9%, albeit poor purification was observed. The recovery yield from solid phase extraction was between 67.75% and 103.08% with RSD value from 0.8% to 14.0%, which met the requirements of trace determination, this method has good precision and stability. These results could be employed to other Traditional Chinese Medicines (TCMs) in detecting prohibited pesticide residues.

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Lycii fructus extract ameliorates hydrogen peroxide-induced cytotoxicity through indirect antioxidant action

Cited by 12 publications

References 21 publications

Modulating effects of oral administration of Lycii Fructus extracts on UVB‑induced skin erythema: A Randomized, placebo‑controlled study

Modulating effects of oral administration of Lycii Fructus extracts on UVB‑induced skin erythema: A Randomized, placebo‑controlled study

Chinese herbal medicine for myasthenia gravis: A systematic review and meta-analysis of randomized clinical trials

A Rapid Determination of 30 Prohibited Pesticide Residues in Lycii Fructus by UPLC-MS/MS

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