Lycopene regulation of cholesterol synthesis and efflux in human macrophages

Palozza, Paola; Simone, Rossella Emanuela; Catalano, Alfonso; Parrone, Nadia; Monego, Giovanni; Ranelletti, Franco O.

doi:10.1016/j.jnutbio.2010.08.010

Cited by 75 publications

(63 citation statements)

References 41 publications

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“…Dulbecco's modified Eagle's medium (DMEM), penicillin, streptomycin, and fetal bovine serum (FBS) were obtained from Gibco (Grand Island, NY); PQ, DA, N‐acetyl‐l‐cysteine (NAC), 4′,6‐diamidino‐2‐phenylindole (DAPI), and N‐benzyloxycarbonyl‐Val‐Ala‐Asp fluorome‐thylketone (z‐VAD‐fmk) were from Sigma‐Aldrich (St. Louis, MO). Trizol reagent and Lipofectamine 2000 were from Invitrogen (Carlsbad, CA); PrimeScript RT Reagent Kit and SYBR Prime EX Taq were from TaKaRa Biotechnology (Shiga, Japan); BCA Protein Assay Kit and ECL Plus reagent were from Beyotime Biotechnology (Beijing, China); the antibodies to β‐actin (SC‐47778; Palozza et al,2011), caspase‐3 (SC‐7148), PARP (SC‐8007), SCG3 (SC‐50289), and CgA (SC‐1488) were from Santa Cruz Biotechnology (Santa Cruz, CA). The MTT cell proliferation and cytotoxicity detection kit was from Keygen Biotechnology (Nanjing, China), and recombinant human caspase‐3, ‐6, and ‐7 were from BioVision.…”

Section: Methodsmentioning

confidence: 99%

Dysregulated expression of secretogranin III is involved in neurotoxin‐induced dopaminergic neuron apoptosis

Tian

Zhou

et al. 2012

J of Neuroscience Research

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The neurotoxins paraquat (PQ) and dopamine (DA or 6-OHDA) cause apoptosis of dopaminergic neurons in the substantia nigra pars compacta (SNpc), reproducing an important pathological feature of Parkinson's disease (PD). Secretogranin III (SCG3), a member of the multifunctional granin family, plays a key role in neurotransmitter storage and transport and in secretory granule biogenesis, which involves the uptake of exogenous toxins and endogenous "toxins" in neuroendocrine cells. However, the molecular mechanisms of neurotoxin-induced apoptosis in dopaminergic neurons and the role of SCG3-associated signaling pathways in neuroendocrine regulation are unclear. To address this, we used PQ- and DA-induced apoptosis in SH-SY5Y human dopaminergic cells as an in vitro model to investigate the association between SCG3 expression level and apoptosis. SCG3 was highly expressed in SH-SY5Y cells, and SCG3 mRNA and protein levels were dramatically decreased after PQ treatment. Apoptosis induced by PQ is associated with caspase activation and decreased SCG3 expression, and restoration of SCG3 expression is observed after treatment with caspase inhibitors. Overexpressed SCG3 in nonneuronal cells and endogenous SCG3 in SH-SY5Y cells are cleaved into specific fragments by recombinant caspase-3 and -7, but the fragments were not detected in PQ-treated SH-SY5Y cells. Therefore, SCG3 may be involved in apoptosis signal transduction as a caspase substrate, leading to loss of its original biological functions. In addition, SCG3 may be a pivotal component of the neuroendocrine pathway and play an important role in neuronal communication and neurotransmitter release, possibly representing a new potential target in the course of PD pathogenesis.

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Section: Methodsmentioning

confidence: 99%

Dysregulated expression of secretogranin III is involved in neurotoxin‐induced dopaminergic neuron apoptosis

Tian

Zhou

et al. 2012

J of Neuroscience Research

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“…Nakaso et al reported that knockdown of Cav-1 and/or Cav-2 prevented the protective effects of tocotrienol in a cellular Parkinson's disease model [169]. In addition, antioxidants such as lycopene [170] and quercetin [171] were also able to induce the expression of Cav-1. In particular, the effects of curcumin on Cav-1 pathway have been deeply reported.…”

Section: Cav-1 and Oxidative Stress Regulationmentioning

confidence: 99%

Caveolin‐1: An Oxidative Stress‐Related Target for Cancer Prevention

Wang

Zheng

et al. 2017

Oxidative Medicine and Cellular Longevity

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Aberrant oxidative metabolism is one of the hallmarks of cancer. Reactive species overproduction could promote carcinogenesis via inducing genetic mutations and activating oncogenic pathways, and thus, antioxidant therapy was considered as an important strategy for cancer prevention and treatment. Caveolin-1 (Cav-1), a constituent protein of caveolae, has been shown to mediate tumorigenesis and progression through oxidative stress modulation recently. Reactive species could modulate the expression, degradation, posttranslational modifications, and membrane trafficking of Cav-1, while Cav-1-targeted treatments could scavenge the reactive species. More importantly, emerging evidences have indicated that multiple antioxidants could exert antitumor activities in cancer cells and protective activities in normal cells by modulating the Cav-1 pathway. Altogether, these findings indicate that Cav-1 may be a promising oxidative stress-related target for cancer antioxidant prevention. Elucidating the underlying interaction mechanisms between oxidative stress and Cav-1 is helpful for enhancing the preventive effects of antioxidants on cancer, for improving clinical outcomes of antioxidant-related therapeutics in cancer patients, and for developing Cav-1 targeted drugs. Herein, we summarize the available evidence of the roles of Cav-1 and oxidative stress in tumorigenesis and development and shed novel light on designing strategies for cancer prevention or treatment by utilizing the interaction mode between Cav-1 and oxidative stress.

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“…Inhibition of agonist-stimulated platelet aggregation have also been observed at physiologically relevant concentrations (73,74) . Experiments carried out in THP-1 (a human monocytic leukemia cell line) macrophages showed that lycopene can inhibit cholesterol synthesis as well as scavenger receptor expression, which suggests that it could potentially modulate foam cell formation (75,76) . Lycopene has potent antioxidant chemical properties, and therefore much interest has focused on its potential ability to inhibit LDL oxidation, which is central to the initiation of atherosclerosis.…”

Section: Mechanistic Studiesmentioning

confidence: 99%

Cardiovascular benefits of lycopene: fantasy or reality?

et al. 2016

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Epidemiological evidence indicates that high consumption of tomatoes and tomato-based products reduces the risk of chronic diseases such as CVD and cancer. Such potential benefits are often ascribed to high concentrations of lycopene present in tomato products. Mainly from the results of in vitro studies, potential biological mechanisms by which carotenoids could protect against heart disease and cancer have been suggested. These include cholesterol reduction, inhibition of oxidation processes, modulation of inflammatory markers, enhanced intercellular communication, inhibition of tumourigenesis and induction of apoptosis, metabolism to retinoids and antiangiogenic effects. However, with regard to CVD, results from intervention studies gave mixed results. Over fifty human intervention trials with lycopene supplements or tomato-based products have been conducted to date, the majority being underpowered. Many showed some beneficial effects but mostly on non-established cardiovascular risk markers such as lipid peroxidation, DNA oxidative damage, platelet activation and inflammatory markers. Only a few studies showed improvement in lipid profiles, C reactive protein and blood pressure. However, recent findings indicate that lycopene could exert cardiovascular protection by lowering HDL-associated inflammation, as well as by modulating HDL functionality towards an antiatherogenic phenotype. Furthermore, in vitro studies indicate that lycopene could modulate T lymphocyte activity, which would also inhibit atherogenic processes and confer cardiovascular protection. These findings also suggest that HDL functionality deserves further consideration as a potential early marker for CVD risk, modifiable by dietary factors such as lycopene.

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Lycopene regulation of cholesterol synthesis and efflux in human macrophages

Cited by 75 publications

References 41 publications

Dysregulated expression of secretogranin III is involved in neurotoxin‐induced dopaminergic neuron apoptosis

Dysregulated expression of secretogranin III is involved in neurotoxin‐induced dopaminergic neuron apoptosis

Caveolin‐1: An Oxidative Stress‐Related Target for Cancer Prevention

Cardiovascular benefits of lycopene: fantasy or reality?

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