Lycopene-rich fractions derived from pink guava by-product and their potential activity towards hydrogen peroxide-induced cellular and DNA damage

Abstract

a b s t r a c tEffects of solvent and supercritical carbon dioxide (SC-CO 2 ) extraction on antioxidant and cytotoxic activities of lycopene-rich fractions of decanted pink guava by-product (decanter) were determined with lycopene-equivalent antioxidant capacity, b-carotene bleaching and MTT (3-(4,5-dimethylthiazol-2yl)-2,5-diphenyl tetrazolium bromide) assays. Extraction with SC-CO 2 gave a higher yield than solvent extraction (3.15 vs. 0.68 mg/100 g dried decanter, corresponding to 42.99 and 33.63 mg of lycopene).No cytotoxicity was found in Chang liver cells supplemented with either extracts (6.25-200 lg/ml). Solvent extract at 25 lg/ml (2.32 lM lycopene) and SC-CO 2 extract at 200 lg/ml (5.09 lM lycopene) had protective effect against hydrogen peroxide-induced cytotoxicity. However, only high concentrations of solvent extract (200 lg/ml; lycopene = 18.65 lM) or lycopene standard (10 lM) protected cells against DNA damage. Supercritical fluid extraction demonstrated a higher yield in lycopene-rich fraction from decanter. These fractions have the potential to be developed as a functional ingredient to prevent oxidative stress and other related diseases.

Effect of lycopene on oxidative stress induced duringd-galactosamine/lipopolysaccharide-sensitized liver injury in rats

“…Numerous studies have indicated the health benefits of consuming lycopene and demonstrated its preventive role in a number of chronic diseases including various types of cancers (Agarwal & Rao, 2000;Bhuvaneswari & Nagini, 2005;Cohen, 2002;Giovannucci, 2002;Khachik et al, 2002). This carotenoid phytonutrient is found to be the most effective antioxidant among all tested carotenes and xanthophylls (Atessahin et al, 2007;Di Mascio et al, 1989;Hekimoglu et al, 2009;Kong et al, 2010). It offers important health benefits, particularly, in regard to prostate, lung, heart, liver and skin health.…”

Lycopene protects against trimethyltin-induced neurotoxicity in primary cultured rat hippocampal neurons by inhibiting the mitochondrial apoptotic pathway

“…Lycopene has been shown to regulate gap-junction communication (Zhang et al, 1991), inhibit cancer cell migration (Bhuvaneswari and Nagini, 2005), arrest cell cycle progression (Ford et al, 2011), and modulate several signal transduction pathways (Palozza et al, 2011). Recently, lycopene has received greater attention as a result of studies indicating that it is a highly efficient antioxidant and free radical scavenger (Atessahin et al, 2007;Hekimoglu et al, 2009;Kong et al, 2010). Lycopene has high liposolubility and so can pass through the blood-brain barrier (Khachik et al, 2002), suggesting it may be a potentially useful treatment for brain diseases associated with oxidative stress.…”

Preparation of Vesicles Entrapped Lycopene Extract

“…To make sure that they were not exposed to light, the cuvettes were covered with an opaque aluminium foil. Antioxidant activity was expressed as percentage scavenging capacity and calculated by using the following equation 10 :…”