Lycorine Hydrochloride Inhibits the Virulence Traits ofCandida albicans

Abstract

The human opportunistic fungal pathogen Candida albicans causes a severe health burden while the biofilms formed by C. albicans present a kind of infections that are hard to cure, highlighting the pressing need for new antifungal drugs against C. albicans. This study was to explore the antifungal activities of lycorine hydrochloride (LH) against C. albicans. The minimal inhibitory concentration (MIC) of LH against C. albicans SC5314 was 64 μM. Below its MIC, LH demonstrated antivirulence property by suppressing adhesion, filamentation, biofilm formation, and development, as well as the production of extracellular phospholipase and exopolymeric substances (EPS). The cytotoxicity of LH against mammalian cells was low, with half maximal inhibitory concentrations (IC50) above 256 μM. Moreover, LH showed a synergistic effect with AmB, although its interaction with fluconazole, as well as caspofungin, was indifferent. Thus, our study reports the potential use of LH, alone or in combination with current antifungal drugs, to fight C. albicans infections.

Fangchinoline inhibits growth and biofilm of Candida albicans by inducing ROS overproduction

“…This is often seen in many antifungal agents. 8,26,32,35 However, the ratio of IC 50 (in biofilm formation assay) to MIC (in planktonic growth) for FN was lower than those reported for other drugs, which were over 1000 for azoles. 8 dismutase 5 (SOD5) is useful to counter the oxidative stress (produced by immune cells or drugs).…”

“…The FN concentration required for about half maximal inhibition (IC 50 ) of biofilm formation was higher than its MIC, and IC 50 for biofilm development was even higher. This is often seen in many antifungal agents 8,26,32,35 . However, the ratio of IC 50 (in biofilm formation assay) to MIC (in planktonic growth) for FN was lower than those reported for other drugs, which were over 1000 for azoles 8 .…”

Small molecule based anti-virulence approaches against Candida albicans infections

“…Lycorine hydrochloride, an alkaloid isolated from the herb Lycoris radiata has proven anti-cancer properties. Notably, it also demonstrates anti-biofilm, anti-filamentation, and anti-adhesive properties against C. albicans interestingly by inhibiting the hyphal formation in varying media conditions such as spider medium, GlcNAC medium, and in RPMI 1640 72 . Piperine, , was shown to inhibit C. albicans biofilm formation, hyphal morphogenesis and disturbs mature biofilms with no effect on C. albicans growth and metabolism.…”

Corrigendum to “Lycorine Hydrochloride Inhibits the Virulence Traits of Candida albicans”

“…In the article titled “Lycorine Hydrochloride Inhibits the Virulence Traits of Candida albicans” [ 1 ], there are minor text changes required in Sections “2.1. Chemicals, Strains, and Growth Conditions” and “2.2.…”