Lycorine inhibits cell proliferation, migration and invasion, and primarily exerts <i>in vitro</i> cytostatic effects in human colorectal cancer via activating the ROS/p38 and AKT signaling pathways

Zhang, Ping; Yuan, Xiaohui; Yu, Tingting; Huang, Huakun; Yang, Chunmei; Zhang, Lulu; Yang, Shen Hsi; Luο, Xingguang; Luo, Jinyong

doi:10.3892/or.2021.7970

Cited by 21 publications

(17 citation statements)

References 40 publications

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“…Numerous studies have explored the anti-tumor activity of LYC, including against cancers such as colorectal, gastric, bladder, lung, breast, melanoma, multiple myeloma, osteosarcoma, and hematological malignancies. The molecular mechanism of the anti-tumor activity of LYC is mainly attributed to the selective induction of apoptosis in cancer cells [13][14][15][16][17][18][19][20][21].…”

Section: Discussionmentioning

confidence: 99%

“…Lycorine (LYC), one component of Amaryllidaceae, is an alkaloid that displays various beneficial functions in some disease models, including anti-inflammation, anti-fibrosis, antiviral, anti-aging, and anti-tumor effects [8][9][10][11][12]. Recently, numerous studies focused on investigating the antitumor feature of LYC in colorectal cancer, gastric cancer, bladder cancer, lung cancer, breast cancer, melanoma, multiple myeloma, osteosarcoma, hematological malignancies [13][14][15][16][17][18][19][20][21]. After administration of LYC, cancer cells died selectively in large numbers because of apoptosis [21].…”

Section: Introductionmentioning

confidence: 99%

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Lycorine ameliorates isoproterenol-induced cardiac dysfunction mainly via inhibiting inflammation, fibrosis, oxidative stress and apoptosis

Jiang

et al. 2021

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Alleviating cardiac dysfunction improves the prognosis of heart failure patients. Lycorine is an alkaloid with several beneficial biological properties. Here, we used mice to evaluate the effect of lycorine on cardiac dysfunction elicited by isoproterenol. Mice were divided into four groups: control, lycorine, isoproterenol, and isoproterenol + lycorine. Mice in the combined group were treated daily with 10 mg/ kg isoproterenol intraperitoneally for 2 weeks and 5 mg/kg lycorine was given simultaneously intraperitoneally for 4 weeks. Cardiac structure and function were assessed by echocardiography, hematoxylin and eosin staining, and Masson's trichrome staining. Isoproterenol-induced cardiac dysfunction and histopathological injury that was significantly improved by treatment with lycorine. Western blotting and the quantitative real-time polymerase chain reaction were used to explore the molecular mechanisms of these effects. Levels of the inflammatory cytokines, interleukin (IL)-1β, IL-6, and tumor necrosis factor-α, were increased by treatment with isoproterenol; these increases were significantly reduced by lycorine, with involvement of the NF-κB signaling pathway. The fibrotic factors, collagen I and collagen III, were increased by isoproterenol and decreased by treatment with lycorine through inhibiting activation of the Smad signaling pathway. In addition, lycorine alleviated oxidative stress as evidenced by a reduction in total reactive oxygen species in the isoproterenol + lycorine group compared to the isoproterenol group. Lycorine exerted an anti-apoptotic effect as evidenced by upregulating Bcl-2 and downregulating Bax. Overall, our findings demonstrate that lycorine protects against cardiac dysfunction induced by isoproterenol by inhibiting inflammation, fibrosis, oxidative stress, and apoptosis.

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Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Lycorine ameliorates isoproterenol-induced cardiac dysfunction mainly via inhibiting inflammation, fibrosis, oxidative stress and apoptosis

Jiang

et al. 2021

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“…Results proved that lycorine at 1.0 mg/kg body weight causes emesis and nausea in animals and humans due to poisoning [ 141 ]. Lycorine as a potential natural candidate for an anticancer drug, was efficient in a very low, single-digit micromolar concentration; the IC50 value generally did not exceed 7.5 μM and it was highly tolerable with negligible toxicity [ 142 , 143 ].…”

Section: Toxicology and Side Effectsmentioning

confidence: 99%

Potential Antiviral Action of Alkaloids

2022

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Viral infections and outbreaks have become a major concern and are one of the main causes of morbidity and mortality worldwide. The development of successful antiviral therapeutics and vaccines remains a daunting challenge. The discovery of novel antiviral agents is a public health emergency, and extraordinary efforts are underway globally to identify safe and effective treatments for different viral diseases. Alkaloids are natural phytochemicals known for their biological activities, many of which have been intensively studied for their broad-spectrum of antiviral activities against different DNA and RNA viruses. The purpose of this review was to summarize the evidence supporting the efficacy of the antiviral activity of plant alkaloids at half-maximum effective concentration (EC50) or half-maximum inhibitory concentration (IC50) below 10 μM and describe the molecular sites most often targeted by natural alkaloids acting against different virus families. This review highlights that considering the devastating effects of virus pandemics on humans, plants, and animals, the development of high efficiency and low-toxicity antiviral drugs targeting these viruses need to be developed. Furthermore, it summarizes the current research status of alkaloids as the source of antiviral drug development, their structural characteristics, and antiviral targets. Overall, the influence of alkaloids at the molecular level suggests a high degree of specificity which means they could serve as potent and safe antiviral agents waiting for evaluation and exploitation.

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“…To date, only few studies have evaluated the potential of Amaryllidaceae alkaloids to combat CRC. Specifically, LYC has been investigated in two studies highlighting its antiproliferative, pro-apoptotic, anti-migratory and anti-invasive properties both in vitro and in vivo [ 37 , 38 ]. PANC was shown to trigger apoptosis via the mitochondrial pathway independent of p53 status [ 39 ].…”

Section: Introductionmentioning

confidence: 99%

Amaryllidaceae Alkaloids Decrease the Proliferation, Invasion, and Secretion of Clinically Relevant Cytokines by Cultured Human Colon Cancer Cells

et al. 2022

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Alkaloids isolated from members of the Amaryllidaceae plant family are promising anticancer agents. The purpose of the current study was to determine if the isocarbostyrils narciclasine, pancratistatin, lycorane, lycorine, crinane, and haemanthamine inhibit phenomena related to cancer progression in vitro. To achieve this, we examined the proliferation, adhesion, and invasion of cultured human colon cancer cells via MTT assay and Matrigel-coated Boyden chambers. In addition, Luminex assays were used to quantify the secretion of matrix metalloproteinases (MMP) and cytokines associated with poor clinical outcomes. We found that all alkaloids decreased cell proliferation regardless of TP53 status, with narciclasine exhibiting the greatest potency. The effects on cell proliferation also appear to be specific to cancer cells. Narciclasine, lycorine, and haemanthamine decrease both adhesion and invasion but with various potencies depending on the cell line. In addition, narciclasine, lycorine, and haemanthamine decreased the secretion of MMP-1, -2, and -7, as well as the secretion of the cytokines pentraxin 3 and vascular endothelial growth factor. In conclusion, the present study shows that Amaryllidaceae alkaloids decrease phenomena and cytokines associated with colorectal cancer progression, supporting future investigations regarding their potential as multifaceted drug candidates.

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Lycorine inhibits cell proliferation, migration and invasion, and primarily exerts in vitro cytostatic effects in human colorectal cancer via activating the ROS/p38 and AKT signaling pathways

Cited by 21 publications

References 40 publications

Lycorine ameliorates isoproterenol-induced cardiac dysfunction mainly via inhibiting inflammation, fibrosis, oxidative stress and apoptosis

Lycorine ameliorates isoproterenol-induced cardiac dysfunction mainly via inhibiting inflammation, fibrosis, oxidative stress and apoptosis

Potential Antiviral Action of Alkaloids

Amaryllidaceae Alkaloids Decrease the Proliferation, Invasion, and Secretion of Clinically Relevant Cytokines by Cultured Human Colon Cancer Cells

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