Lycorine inhibits MDA-MB-231 breast cancer cell proliferation, migration and invasion that are associated with Wnt/β-catenin signaling

Abstract

Lycorine inhibits MDA-MB-231 breast cancer cell proliferation, migration and invasion that are associated with Wnt/β-catenin signaling BJP IntroductionBreast cancer is the second leading cause of cancer death among women after lung cancer in the United States (DeSantis et al., 2014). In the treatment for breast cancer, some adverse effects could cause serious complications and affect the health and quality of life among survivors (Odle, 2014). Therefore, it is necessary to develop novel anti-cancer candidates for breast cancer.In the past several decades, natural compounds such as resveratrol (Sinha et al., 2016), epigallocatechin-3-gallate, oldenlandia diffusa and Ziziphus jujube have been demonstrated that possessed potential anti-breast cancer activity (Bonofiglio et al., 2016).Lycorine, known as a multifunctional compound, is extensively studied for its pharmacological activities, such as anti-tumor activity (Lamoral-Theys et al., 2010). Previous studies have shown that lycorine presented a potential anti-cancer activity in breast cancer via the inhibition of tumor growth and metastasis Ying et al., 2017). Src/FAK (focal adhesion kinase) -involved pathway and STAT3 (signal transducer and activator of transcription 3) signaling pathway were involved in growth, invasion and metastasis of breast cancer.However, Wnt/ -catenin signaling, a multifunctional pathway that has not been studied to be involved in proliferation, invasion and migration of breast cancer. This study is meant to confirm the inhibitory effects of lycorine on the proliferation, migration and invasion of MDA-MB-231 breast cancer cells and investigate the activation of Wnt/ -catenin signaling pathway involved. Materials and Methods Cell lines and reagentsHuman MDA-MB-231 breast cancer cells were obtained AbstractThis study was aimed to determine the effects of lycorine, a toxic crystalline alkaloid, on MDA-MB-231 breast cancer cells proliferation, migration and invasion, and to investigate the mechanism involved. The cells were cultured with different concentrations of lycorine in vitro. MTT assays were performed to determine the proliferation of cells. Transwell assays were performed to measure the migration and invasion of cells. The activation of Wnt/ -catenin signaling pathway and expression were assayed by Western blot. This study showed that proliferation, migration and invasion of MDA-MB-231 breast cancer cells could be inhibited by lycorine. Furthermore, we found that Wnt/ -catenin signaling was markedly blocked in MDA-MB-321 cells treated with lycorine. In conclusion, lycorine inhibits the proliferation, migration and invasion of MDA-MB-231 breast cancer cells that is associated with the suppression of Wnt/ -catenin signaling. Article Info