Lyme disease in Bernese Mountain Dogs. Is it a real problem?

Adaszek, Łukasz; Pisarek, M; Kalinowski, M.; Skrzypczak, Maciej; Winiarczyk, Mateusz; Abramowicz, Beata; Winiarczyk, S.

doi:10.24425/pjvs.2022.142036

Polish Journal of Veterinary Sciences

2022

DOI: 10.24425/pjvs.2022.142036

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Lyme disease in Bernese Mountain Dogs. Is it a real problem?

Łukasz Adaszek

M Pisarek

M. Kalinowski

et al.

Abstract: Borreliosis is the most frequently diagnosed tick-borne disease caused by spirochete bacteria belonging to the genus Borreliae -Borrelia burgdorferi sensu stricto (s.s.), Borrelia afzelii and Borrelia garinii. Clinical manifestations in dogs include fever, lameness, polyarthritis and glomerulonephritis. Diagnosis is mainly serological and is based on an immunoenzymatic test followed by a Western blot confirmatory test. Early treatment with antibiotics such as doxycycline or amoxicillin, for four weeks, usually… Show more

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Cited by 3 publications

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Lyme disease in companion animals: an updated state-of-art and current situation in Portugal

Picado,

Baptista,

Meneses

et al. 2024

Vet Res Commun

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Lyme disease (LD) is a globally distributed zoonotic multisystemic condition caused by gram-negative spirochete bacteria of the Borrelia burgdorferi complex, transmitted through tick bites. Research on LD in domestic animals in Portugal is limited, potentially leading to underestimating its prevalence. This disease affects many species, including humans, making it a critical public health issue. In domestic animals, LD often presents subclinically or with non-specific clinical signs, complicating its diagnosis. Nevertheless, veterinarians should always consider LD in cases with a history of tick exposure and compatible clinical signs. Diagnostic confirmation can be achieved through serological and other complementary tests. Treatment involves eradicating the bacterial infection and managing clinical signs using a combination of antibiotics, analgesics, anti-inflammatories, and other medications. Effective prevention primarily relies on tick control measures. This review aims to provide an up-to-date state-of-the-art LD, particularly in Portugal.

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Lyme disease in companion animals: an updated state-of-art and current situation in Portugal

Picado,

Baptista,

Meneses

et al. 2024

Vet Res Commun

View full text Add to dashboard Cite

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Screening Marine Microbial Metabolites as Promising Inhibitors of Borrelia garinii: A Structural Docking Approach towards Developing Novel Lyme Disease Treatment

Basharat,

Sattar,

Bahauddin

et al. 2024

BioMed Research International

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Lyme disease caused by the Borrelia species is a growing health concern in many parts of the world. Current treatments for the disease may have side effects, and there is also a need for new therapies that can selectively target the bacteria. Pathogens responsible for Lyme disease include B. burgdorferi, B. afzelii, and B. garinii. In this study, we employed structural docking-based screening to identify potential lead-like inhibitors against the bacterium. We first identified the core essential genome fraction of the bacterium, using 37 strains. Later, we screened a library of lead-like marine microbial metabolites (n=4730) against the arginine deiminase (ADI) protein of Borrelia garinii. This protein plays a crucial role in the survival of the bacteria, and inhibiting it can kill the bacterium. The prioritized lead compounds demonstrating favorable binding energies and interactions with the active site of ADI were then evaluated for their drug-like and pharmacokinetic parameters to assess their suitability for development as drugs. Results from molecular dynamics simulation (100 ns) and other scoring parameters suggest that the compound CMNPD18759 (common name: aureobasidin; IUPAC name: 2-[(4R,6R)-4,6-dihydroxydecanoyl]oxypropan-2-yl (3S,5R)-3,5-dihydroxydecanoate) holds promise as a potential drug candidate for the treatment of Lyme disease, caused by B. garinii. However, further experimental studies are needed to validate the efficacy and safety of this compound in vivo.

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Diagnostic value of PCR analysis of synovial fluid for the diagnosis of Lyme borreliosis in dogs

Panteleienko,

Tsarenko

2023

Nauk. vìsn. vet. med.

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Lyme borreliosis, also known as Lyme disease, is a chronic multiorgan disease of humans and animals transmitted by ticks of the Ixodidae family and caused by a group of spirochetes Borrelia burgdorferi sensu lato. Dogs are one of the most susceptible animal species to Lyme disease. Symptoms of the disease in dogs can range from mild lameness and fever to more severe and potentially life-threatening conditions, such as kidney damage and neurological disorders. Due to the variety of clinical manifestations and the lack of a universal diagnostic approach, veterinarians often face difficulties in accurately diagnosing the disease. Therefore, improving the algorithms for diagnosing Lyme borreliosis in dogs remains an urgent issue for practical veterinary medicine. The article describes and analyzes data on two cases of dogs from Kyiv (Ukraine) that presented with musculoskeletal disorders, including lameness, swelling of the knee joints, and tenderness, which raised suspicion of Lyme borreliosis. The diagnostic tests used to confirm the diagnosis of Lyme borreliosis included clinical blood test, C-reactive protein test, cerebrospinal fluid cytology, enzyme-linked immunosorbent assay for IgM and IgG antigens to Borrelia burgdorferi sensu lato, computed tomography and PCR analysis of synovial fluid for the presence of Borrelia burgdorferi sensu lato DNA. As a result, the clinical blood test, C-reactive protein test, and cerebrospinal fluid cytology were not diagnostically important for the diagnosis of Lyme borreliosis. In both cases, serological tests for antibodies to Borrelia burgdorferi sensu lato were not positive. Computed tomography showed the development of an inflammatory process in the knee joints with the formation of osteophytes, enthesophytes, sclerosis of the articular surfaces, and an increase in the volume of synovial bursa. The final diagnosis of Lyme arthritis in both dogs was established by PCR analysis of synovial fluid from the affected joints for the presence of Borrelia burgdorferi sensu lato DNA. This study emphasizes the importance of considering Lyme borreliosis as a potential cause of musculoskeletal disorders in dogs. We also propose an expanded algorithm for the diagnosis of Lyme borreliosis in dogs with a pronounced symptom complex of Lyme arthritis using the method of PCR analysis of synovial fluid to detect the DNA of Borrelia burgdorferi sensu lato spirochetes. Key words: Lyme borreliosis in dogs, clinical cases, diagnosis, polymerase chain reaction, Borrelia burgdorferi sensu lato.

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Lyme disease in Bernese Mountain Dogs. Is it a real problem?

Cited by 3 publications

References 42 publications

Lyme disease in companion animals: an updated state-of-art and current situation in Portugal

Lyme disease in companion animals: an updated state-of-art and current situation in Portugal

Screening Marine Microbial Metabolites as Promising Inhibitors of Borrelia garinii: A Structural Docking Approach towards Developing Novel Lyme Disease Treatment

Diagnostic value of PCR analysis of synovial fluid for the diagnosis of Lyme borreliosis in dogs

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