Lymnaea stagnalis as a freshwater model invertebrate for ecotoxicological studies

Amorim, João; Abreu, Ilda; Rodrigues, Pedro Pereira; Peixoto, Diogo; Pinheiro, Carlos; Saraiva, Aurélia; Carvalho, António Paulo; Guimarães, Laura; Oliva-Teles, Luís

doi:10.1016/j.scitotenv.2019.03.035

Cited by 82 publications

(43 citation statements)

References 120 publications

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“…Marine bivalves such as A. islandica and P. maximus, for which we reported successful aDNA recovery in this study, have previously allowed sclerochronological reconstructions of past seawater temperature and salinity changes at millenniallength annual and seasonal resolutions, respectively (Surge et al, 2003;Chauvaud et al, 2012;Vokhshoori and McCarthy, 2014;Reynolds et al, 2016;Black et al, 2017). At a more local scale, the freshwater L. stagnalis, for which we report shell aDNA recovery here for the first time, is a model commonly used in ecotoxicology (Amorim et al, 2019). Its abundant and widespread distribution in temperate limnic systems and its low dispersal ability can be leveraged to investigate the impact of pollutants in freshwater systems, in particular pesticides, molluscicides, algaecides or industrial (petro-) chemicals pollutants (Amorim et al, 2019).…”

Section: Potential Of Ancient Mollusk Dna For Studying Environmental mentioning

confidence: 55%

“…At a more local scale, the freshwater L. stagnalis, for which we report shell aDNA recovery here for the first time, is a model commonly used in ecotoxicology (Amorim et al, 2019). Its abundant and widespread distribution in temperate limnic systems and its low dispersal ability can be leveraged to investigate the impact of pollutants in freshwater systems, in particular pesticides, molluscicides, algaecides or industrial (petro-) chemicals pollutants (Amorim et al, 2019). Adding temporally sampled genomic data would allow for controlling the genomic background of L. stagnalis population subjected to toxic stress and provide baselines prior to the use of pesticides and chemicals (Coutellec et al, 2013;Bouétard et al, 2014).…”

Section: Potential Of Ancient Mollusk Dna For Studying Environmental mentioning

confidence: 98%

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Unveiling the Ecological Applications of Ancient DNA From Mollusk Shells

et al. 2020

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The shells of marine mollusks represent promising metagenomic archives of the past, adding to bones, teeth, hairs, and environmental samples most commonly examined in ancient DNA research. Seminal work has established that DNA recovery from marine mollusk shells depends on their microstructure, preservation and disease state, and that authentic ancient DNA could be retrieved from specimens as old as 7,000 years. Here, we significantly push the temporal limit for shell DNA recovery to ≥100,000 years with the successful genetic characterization of one Portlandia arctica and one Mytilus mussel sample collected within a dated permafrost layer from the Taimyr Peninsula, Russia. We expand the analysis of ancient DNA in carbonate shells to a larger number of genera (Arctica, Cernuella, Crassostrea, Dreissena, Haliotis, Lymnaea, Margaritifera, Pecten, Ruditapes, Venerupis) from marine, freshwater and terrestrial environments. We demonstrate that DNA from ancient shells can provide sufficient resolution for taxonomic, phylogenetic and/or population assignment. Our results confirm mollusk shells as long-term DNA reservoirs, opening new avenues for the investigation of environmental changes, commercial species management, biological invasion, and extinction. This is especially timely in light of modern threats to biodiversity and ecosystems.

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Section: Potential Of Ancient Mollusk Dna For Studying Environmental mentioning

confidence: 55%

Section: Potential Of Ancient Mollusk Dna For Studying Environmental mentioning

confidence: 98%

Unveiling the Ecological Applications of Ancient DNA From Mollusk Shells

et al. 2020

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“…Lymnaea stagnalis is also a popular model organism for studies of ecotoxicology [57,58] and a bioindicator of aquatic contaminants [57]. Toxicity studies using L. stagnalis began to appear in the late the 1970s, however, it is after the late 1990s that a great number of toxicological investigations focused on the sensitivity to metals, e.g., aluminum, mercury, cadmium, and particularly to lead [59].…”

Section: Ecotoxicologymentioning

confidence: 99%

The pond snail Lymnaea stagnalis

Kuroda

Abe

2020

EvoDevo

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The freshwater snail Lymnaea stagnalis has a long research history, but only relatively recently has it emerged as an attractive model organism to study molecular mechanisms in the areas of developmental biology and translational medicine such as learning/memory and neurodegenerative diseases. The species has the advantage of being a hermaphrodite and can both cross- and self-mate, which greatly facilitates genetic approaches. The establishment of body-handedness, or chiromorphogenesis, is a major topic of study, since chirality is evident in the shell coiling. Chirality is maternally inherited, and only recently a gene-editing approach identified the actin-related gene Lsdia1 as the key handedness determinant. This short article reviews the natural habitat, life cycle, major research questions and interests, and experimental approaches.

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“…In general, there is a diverse literature on the reproductive and developmental effects as well as the induced behavioural responses of potential endocrine disruptive PhACs including progestogens in invertebrates. Furthermore, there is a longstanding debate about whether, or not, natural vertebrate and synthetic sex steroid residues occurring in the environment can affect the neuroendocrine system and physiological processes of molluscan species (Alzieu 2000;Amorim et al 2019;Fodor et al 2020b;Matthiessen and Gibbs 1998;Scott 2012Scott , 2013Scott , 2018Tran et al 2019). Previous studies demonstrated that three key steps-cholesterol side-chain cleavage, 17-hydroxylation, and aromatisation-of the classical vertebrate steroid biosynthetic pathway are either absent, or occur very weakly, in molluscs (reviewed in Fodor et al 2020b;Scott 2012).…”

Section: Progestogens-induced Alterations In L Stagnalismentioning

confidence: 99%

Progestogen-induced alterations and their ecological relevance in different embryonic and adult behaviours of an invertebrate model species, the great pond snail (Lymnaea stagnalis)

Svigruha

Fodor

Padisák

et al. 2020

Environ Sci Pollut Res

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The presence of oral contraceptives (basically applying estrogens and/or progestogens) poses a challenge to animals living in aquatic ecosystems and reflects a rapidly growing concern worldwide. However, there is still a lack in knowledge about the behavioural effects induced by progestogens on the non-target species including molluscs. In the present study, environmental progestogen concentrations were summarised. Knowing this data, we exposed a well-established invertebrate model species, the great pond snail (Lymnaea stagnalis) to relevant equi-concentrations (1, 10, 100, and 500 ng L−1) of mixtures of four progestogens (progesterone, drospirenone, gestodene, levonorgestrel) for 21 days. Significant alterations were observed in the embryonic development time, heart rate, feeding, and gliding activities of the embryos as well as in the feeding and locomotion activity of the adult specimens. All of the mixtures accelerated the embryonic development time and the gliding activity. Furthermore, the 10, 100, and 500 ng L−1 mixtures increased the heart rate and feeding activity of the embryos. The 10, 100, and 500 ng L−1 mixtures affected the feeding activity as well as the 1, 10, and 100 ng L−1 mixtures influenced the locomotion of the adult specimens. The differences of these adult behaviours showed a biphasic response to the progestogen exposure; however, they changed approximately in the opposite way. In case of feeding activity, this dose-response phenomenon can be identified as a hormesis response. Based on the authors’ best knowledge, this is the first study to investigate the non-reproductive effects of progestogens occurring also in the environment on molluscan species. Our findings contribute to the global understanding of the effects of human progestogens, as these potential disruptors can influence the behavioural activities of non-target aquatic species. Future research should aim to understand the potential mechanisms (e.g., receptors, signal pathways) of progestogens induced behavioural alterations.

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Lymnaea stagnalis as a freshwater model invertebrate for ecotoxicological studies

Cited by 82 publications

References 120 publications

Unveiling the Ecological Applications of Ancient DNA From Mollusk Shells

Unveiling the Ecological Applications of Ancient DNA From Mollusk Shells

The pond snail Lymnaea stagnalis

Progestogen-induced alterations and their ecological relevance in different embryonic and adult behaviours of an invertebrate model species, the great pond snail (Lymnaea stagnalis)

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