Lymph node extraction of radiopaque nanoparticulates in the rabbit as measured in vivo with CT

Wolf, Gerald; Shore, Mary Theresa; Bessin, Geoffrey; McIntire, Gregory L.; Bacon, Edward R.; Illig, Kathleen J.

doi:10.1016/s1076-6332(99)80062-2

Cited by 12 publications

(3 citation statements)

References 22 publications

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“…Functional studies of lymphatic vessels in mice have been extremely difficult to perform because of the low spatial resolution and the insufficient signal intensity imposed by most currently used methods. By using computed tomography and a recently developed lymphographic contrast agent with an increased concentration of iodine that results in image enhancement, 41 we have established a simple, minimally invasive procedure to measure the efficacy of lymphatic transport in mice. In comparison to control tumors, lymphatic transport from MeWo/VEGF-C tumors to regional lymph nodes was markedly retarded, indicating functional impairment of lymphatic vessels in terms of transport or uptake of fluid or functional overload because of an increase in VEGF-C-induced vascular permeability.…”

Section: Discussionmentioning

confidence: 99%

“…Three mice carrying two tumors each were analyzed in both groups. After anesthesia with a mixture of ketamine (100 mg/kg) and xylazine (10 mg/kg), mice were injected with an iodinated lymphographic contrast agent, 41 and imaging was performed by using a helical computed tomography scanner (TCT900S/xII; Toshiba, Tokyo, Japan). The contrast agent was administered very slowly by injection into the center of each tumor (20 l per tumor) by using a 30-gauge needle attached to a Hamilton syringe.…”

Section: Computed Tomographymentioning

confidence: 99%

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Concurrent Induction of Lymphangiogenesis, Angiogenesis, and Macrophage Recruitment by Vascular Endothelial Growth Factor-C in Melanoma

Skobe

Hamberg

Hawighorst

et al. 2001

The American Journal of Pathology

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350

322

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Interactions of tumor cells with lymphatic vessels are of paramount importance for tumor progression, however, the underlying molecular mechanisms are poorly understood. Whereas enlarged lymphatic vessels are frequently observed at the periphery of malignant melanomas, it has remained unclear whether intratumoral lymphangiogenesis occurs within these tumors. Here, we demonstrate the presence of intratumoral lymphatics and enlargement of lymphatic vessels at the tumor periphery in vascular endothelial growth factor (VEGF)-C-overexpressing human melanomas transplanted onto nude mice. VEGF-C expression also resulted in enhanced tumor angiogenesis, indicating a coordinated regulation of lymphangiogenesis and angiogenesis in melanoma progression. The specific biological effects of VEGF-C were critically dependent on its proteolytic processing in vivo.

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Section: Discussionmentioning

confidence: 99%

Section: Computed Tomographymentioning

confidence: 99%

Concurrent Induction of Lymphangiogenesis, Angiogenesis, and Macrophage Recruitment by Vascular Endothelial Growth Factor-C in Melanoma

Skobe

Hamberg

Hawighorst

et al. 2001

The American Journal of Pathology

Self Cite

350

322

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“…Replacing an intraperitoneally injected microsuspension by a nanosuspension could avoid irritation of the peritoneum (unpublished data). Literature is enriched with preclinical studies reports for subcutaneous application of nanocrystal formulations (Wolf et al 1999). However, by now these routes are not intensively studied, almost nothing is published.…”

Section: Parenteral Deliverymentioning

confidence: 99%

Application of Nanocrystals in Drug Delivery: An Overview

Dahiya¹

2018

Bull. Pharm. Res.

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Poor aqueous solubility has always been remained a challenge for delivery of drugs and show dissolution rate limited absorption. This problem seeks more attention in current time where number of poorly soluble candidates have increased up to 70 percent or more. Although extensive research efforts led to many successful marketed products using some commonly used solubility enhancement techniques such as micronization, salt formation, solid dispersion and complexation etc., each of the technique is associated with its own limitations. Nanonization approach involves transfer of poorly soluble materials into the nanodimension that changes their physical properties, and was utilized in pharmaceutical development of an innovative technology; known as drug nanocrystals. Among various nanotechnological approaches, nanocrystals have been emerged as one of the potential technology to overcome issues related to poor water solubility of drugs and its in vivo consequences. This article overviews the feasibility of nanocrystals for administration of poorly soluble drugs by oral, parenteral, dermal, mucosal, pulmonary, ocular and targeted delivery.

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Nanocrystals: Industrially feasible multifunctional formulation technology for poorly soluble actives

Shegokar

Müller

2010

International Journal of Pharmaceutics

640

307

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Lymph node extraction of radiopaque nanoparticulates in the rabbit as measured in vivo with CT

Cited by 12 publications

References 22 publications

Concurrent Induction of Lymphangiogenesis, Angiogenesis, and Macrophage Recruitment by Vascular Endothelial Growth Factor-C in Melanoma

Concurrent Induction of Lymphangiogenesis, Angiogenesis, and Macrophage Recruitment by Vascular Endothelial Growth Factor-C in Melanoma

Application of Nanocrystals in Drug Delivery: An Overview

Nanocrystals: Industrially feasible multifunctional formulation technology for poorly soluble actives

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