Lymphadenopathy Caused by
            <i>Mycobacterium colombiense</i>

Esparcia, Óscar; Navarro, Ferrán; Quer, Miquel; Coll, Pere

doi:10.1128/jcm.01441-07

Cited by 34 publications

(30 citation statements)

References 14 publications

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“…We did not have additional clinical data to confirm the clinical significance; however, these data suggest the possibility of disseminated infection due to M. chimaera. We did not identify M. colombiense, a newly described MAC species isolated from the blood and sputum specimens of HIV-infected patients in Colombia (Murcia et al, 2006) and in one case of lymphadenopathy in a 3-year-old Spanish girl (Esparcia et al, 2008).…”

Section: Discussionmentioning

confidence: 99%

rpoB sequence-based identification of Mycobacterium avium complex species

et al. 2008

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The Mycobacterium avium complex (MAC) comprises slowly growing mycobacteria responsible for opportunistic infections and zoonoses. The ability to speciate MAC isolates in the clinical microbiology laboratory is critical for determining the organism implicated in clinical disease and for epidemiological investigation of the source of infection. Investigation of a 711 bp variable fragment of rpoB flanked by the Myco-F/Myco-R primers found a 0.7-5.1 % divergence among MAC reference strains, with Mycobacterium chimaera and Mycobacterium intracellulare being the most closely related. Using a 0.7 % divergence cut-off, 83 % of 100 clinical isolates, which had been previously identified by phenotypic characteristics and 16S-23S rDNA intergenic spacer (ITS) probing, were identified as M. avium, 8 % as M. intracellulare and 2 % as M. chimaera. The uniqueness of seven isolates, exhibiting ,99.3 % rpoB sequence similarity with MAC reference strains, was confirmed by 16S rDNA, ITS and hsp65 sequencing and phylogenetic analyses. Partial rpoB gene sequencing using the Myco-F/Myco-R primers permits one-step identification of MAC isolates at the species level and the detection of potentially novel MAC species.

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Section: Discussionmentioning

confidence: 99%

rpoB sequence-based identification of Mycobacterium avium complex species

et al. 2008

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“…Although M. chimaera has been assumed to be highly virulent (7), a recent study in Germany found only 3.3% of 90 M. chimaera isolates to be clinically relevant (10). M. colombiense was fi rst described as a causative agent of mostly disseminated disease in HIV patients from Colombia (11) and was recently isolated from a child with lymphadenopathy in Spain (12). Its isolation in other countries and from respiratory samples in HIV-negative patients has not been reported.…”

Section: Discussionmentioning

confidence: 99%

Clinical Relevance of Nontuberculous Mycobacteria, Oman

Al-Mahruqi¹,

Ingen²,

Al-Busaidy³

et al. 2009

Emerg. Infect. Dis.

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Little is known about the clinical relevance of nontuberculous mycobacteria (NTM) in the Arabian Peninsula. We assessed the prevalence and studied a random sample of isolates at a reference laboratory in Muscat, Oman. NTM cause disease in this region, and their prevalence has increased.N ontuberculous mycobacteria (NTM) are common inhabitants of the environment and have been cultured from water, soil, and animal sources worldwide. NTM are opportunistic pathogens, mostly affecting patients with preexisting pulmonary disease such as chronic obstructive pulmonary disease or tuberculosis (TB), or those with systemic impairment of immunity. The latter group includes those with HIV infection, those using immunosuppressive drugs, and those with leukemia (1).Because NTM are common in the environment and resistant to commonly used disinfectants, they can be present in nonsterile patient material such as sputum and contaminated medical equipment (bronchoscope washers or samples in the laboratory) and consequently cause pseudoinfection (1,2). To distinguish pseudoinfections from NTM disease and establish the clinical relevance of isolated NTM, the American Thoracic Society (ATS) has published diagnostic criteria (1).Studies on the clinical relevance of NTM have traditionally been restricted to western countries, where the incidence of TB is relatively low. More recently, research has been initiated in African and East Asian countries (1-5). There have been no recent reports on isolation and clinical relevance of NTM on the Arabian Peninsula. We analyzed a random sample of NTM isolated from clinical samples in Oman by using molecular techniques. Their clinical relevance was retrospectively analyzed by applying the updated ATS diagnostic criteria for NTM disease (1). The StudyPrevalence of NTM at the Central Public Health Laboratory (CPHL), the national TB reference laboratory of the Ministry of Health in Muscat, Oman, was assessed by using a laboratory database. The CPHL Institutional Review Board reviewed and approved this study. The CPHL received 5,488 samples submitted for Mycobacterium spp. culture during 2006-2007. Samples were subcultured in Lowenstein-Jensen medium and automated Mycobacterial Growth Indicator Tubes (MGIT960; Becton Dickinson, Franklin Lakes, NJ, USA) and incubated at 36°C. A total of 491 (9%) samples yielded positive cultures. M. tuberculosis complex bacteria were isolated from 445 (91%) samples, and NTM were isolated from 46 samples (9%). Most NTM were cultured from respiratory samples (sputum, n = 36, 78%; bronchial lavage, n = 2, 4%); the remainder were from lymph nodes (n = 3, 6%), urine (n = 2, 4%), or other sources (n = 3, 6%). The percentage of NTM increased from 7.6% (18/235) in 2006 to 10.9% (28/256) in 2007.Thirteen samples were randomly selected from all NTM isolated at CPHL during January 2006-September 2007. Selected NTM were subjected to molecular identification at the Dutch National Mycobacteria Reference Laboratory (National Institute for Public Health and the Environment, Bilthoven, ...

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“…Mycobacterium colombiense is a MAC species that was isolated from the sputum and blood of HIV/AIDS Colombian patients (28); this strain produced lymphadenopathy in immunocompetent children from France and Spain (29,30) and was associated with pulmonary infections that complicated cases of cystic fibrosis (31). Therefore, this group of opportunistic pathogens have virulence mechanisms that allow them to adapt, survive, replicate, and produce disease in the host.…”

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confidence: 99%

Virulence and Immune Response Induced by Mycobacterium avium Complex Strains in a Model of Progressive Pulmonary Tuberculosis and Subcutaneous Infection in BALB/c Mice

et al. 2013

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eThe genus Mycobacterium comprises more than 150 species, including important pathogens for humans which cause major public health problems. The vast majority of efforts to understand the genus have been addressed in studies with Mycobacterium tuberculosis. The biological differentiation between M. tuberculosis and nontuberculous mycobacteria (NTM) is important because there are distinctions in the sources of infection, treatments, and the course of disease. Likewise, the importance of studying NTM is not only due to its clinical significance but also due to the mechanisms by which some species are pathogenic while others are not. Mycobacterium avium complex (MAC) is the most important group of NTM opportunistic pathogens, since it is the second largest medical complex in the genus after the M. tuberculosis complex. Here, we evaluated the virulence and immune response of M. avium subsp. avium and Mycobacterium colombiense, using experimental models of progressive pulmonary tuberculosis and subcutaneous infection in BALB/c mice. Mice infected intratracheally with a high dose of MAC strains showed high expression of tumor necrosis factor alpha (TNF-␣) and inducible nitric oxide synthase with rapid bacillus elimination and numerous granulomas, but without lung consolidation during late infection in coexistence with high expression of anti-inflammatory cytokines. In contrast, subcutaneous infection showed high production of the proinflammatory cytokines TNF-␣ and gamma interferon with relatively low production of anti-inflammatory cytokines such as interleukin-10 (IL-10) or IL-4, which efficiently eliminate the bacilli but maintain extensive inflammation and fibrosis. Thus, MAC infection evokes different immune and inflammatory responses depending on the MAC species and affected tissue.

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Lymphadenopathy Caused by Mycobacterium colombiense

Cited by 34 publications

References 14 publications

rpoB sequence-based identification of Mycobacterium avium complex species

rpoB sequence-based identification of Mycobacterium avium complex species

Clinical Relevance of Nontuberculous Mycobacteria, Oman

Virulence and Immune Response Induced by Mycobacterium avium Complex Strains in a Model of Progressive Pulmonary Tuberculosis and Subcutaneous Infection in BALB/c Mice

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