Lymphangiogenesis in Canine Mammary Tumours: A Morphometric and Prognostic Study

Sleeckx, N.; Brantegem, Leen Van; Eynden, G. Van den; Fransén, Erik; Casteleyn, Christophe; Cruchten, Steven Van; Kroeze, Edwin Veldhuis; Ginneken, Chris Van

doi:10.1016/j.jcpa.2013.09.006

Cited by 14 publications

(4 citation statements)

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“…; Sleeckx et al . ). In addition, the upregulation of VEGFR‐2 was also observed in canine SCC Al‐Dissi et al .…”

Section: Introductionmentioning

confidence: 97%

“…However, although several studies have reported on the expression of angiogenesisrelated genes in canine tumours, few studies have compared the expression profiles of angiogenesisrelated genes in normal and tumour tissues. It was reported that the expression of VEGF-A was correlated with malignant progression of canine squamous cell carcinomas (SCC) and seminomas (Al-Dissi et al 2007;Sleeckx et al 2014). In addition, the upregulation of VEGFR-2 was also observed in canine SCC Al-Dissi et al 2007.…”

Section: Introductionmentioning

confidence: 99%

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Angiogenesis‐related gene expression profile in clinical cases of canine cancer

Tanabe

Kobayashi

Maeda

et al. 2018

Veterinary Medicine & Sci

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The balance between pro‐ and anti‐angiogenic signalling is tightly regulated in normal tissues to maintain the functions of the vasculature. In contrast, the overproduction of angiogenic factors and enhanced angiogenesis are frequently observed in several types of tumours. Although there have been many reports on the correlation between tumour progression and angiogenesis in humans, little is known about tumour angiogenesis in canines. Hence, we attempted to clarify whether angiogenesis contributes to tumour progression in canines as well as humans. In this study, we investigated the expression of several angiogenesis‐related genes, including CD 34 , VEGF ‐A , VEGFR ‐1 , VEGFR ‐2 , Ang‐1 , Ang‐2 , Tie1, and Tie2 , in 66 canine tumour tissues and in the normal tissues surrounding the tumours by quantitative real‐time PCR analysis. Our comparative analysis between canine tumour tissues and normal tissues revealed that several angiogenesis‐related genes, such as vascular endothelial growth factor ( VEGF ) and VEGF ‐receptor genes, were significantly upregulated in canine tumour tissues when compared to the normal tissues. We also found that the angiopoietin ( Ang ) ‐1/Ang‐2 gene expression ratio was lower in canine tumour tissues than in the normal tissues, suggesting less association between vascular endothelial cells and perivascular cells in the canine tumour tissues. Taken together, our results suggest that several angiogenesis‐related genes may contribute to the malignant progression of canine tumours via tumour angiogenesis.

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“…; Sleeckx et al . ). In addition, the upregulation of VEGFR‐2 was also observed in canine SCC Al‐Dissi et al .…”

Section: Introductionmentioning

confidence: 97%

Section: Introductionmentioning

confidence: 99%

Angiogenesis‐related gene expression profile in clinical cases of canine cancer

Tanabe

Kobayashi

Maeda

et al. 2018

Veterinary Medicine & Sci

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“…2024, 11, 238 2 of 23 resistance [12,13]. Furthermore, CMTs have been suggested as a model to study the pathogenesis and prognosis of HBC [14][15][16].…”

Section: Introductionmentioning

confidence: 99%

“…Notably, CMTs and human breast cancer (HBC) share a significant number of similarities in pathogenic factors, histological characteristics, molecular phenotypes, biological behaviors, and modes of metastasis [ 10 , 11 ], such as acquired chemotherapeutic resistance [ 12 , 13 ]. Furthermore, CMTs have been suggested as a model to study the pathogenesis and prognosis of HBC [ 14 , 15 , 16 ].…”

Section: Introductionmentioning

confidence: 99%

Fascin-1 Promotes Cell Metastasis through Epithelial–Mesenchymal Transition in Canine Mammary Tumor Cell Lines

Wang,

Zhou,

Wang

et al. 2024

Veterinary Sciences

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Canine mammary tumors (CMTs) are the most common type of tumor in female dogs. In this study, we obtained a metastatic key protein, Fascin-1, by comparing the proteomics data of in situ tumor and metastatic cell lines from the same individual. However, the role of Fascin-1 in the CMT cell line is still unclear. Firstly, proteomics was used to analyze the differential expression of Fascin-1 between the CMT cell lines CHMm and CHMp. Then, the overexpression (CHMm-OE and CHMp-OE) and knockdown (CHMm-KD and CHMp-KD) cell lines were established by lentivirus transduction. Finally, the differentially expressed proteins (DEPs) in CHMm and CHMm-OE cells were identified through proteomics. The results showed that the CHMm cells isolated from CMT abdominal metastases exhibited minimal expression of Fascin-1. The migration, adhesion, and invasion ability of CHMm-OE and CHMp-OE cells increased, while the migration, adhesion, and invasion ability of CHMm-KD and CHMp-KD cells decreased. The overexpression of Fascin-1 can upregulate the Tetraspanin 4 (TSPAN4) protein in CHMm cells and increase the number of migrations. In conclusion, re-expressed Fascin-1 could promote cell EMT and increase lamellipodia formation, resulting in the enhancement of CHMm cell migration, adhesion, and invasion in vitro. This may be beneficial to improve female dogs’ prognosis of CMT.

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