Lymphangiogenesis in human dental pulp

Pimenta, Flávio Juliano; Sa, Afanas'ev; Gomez, Ricardo Santiago

doi:10.1111/j.1365-2591.2003.00728.x

Cited by 19 publications

(18 citation statements)

References 18 publications

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“…The results obtained in our study (42 ± 21 per 1 mm 2 ) did not agree with other authors' results: 90 ± 27.5 in healthy tissue and 56.7 ± 21.2 in tissue with irreversible inflammation [17] along with 19.7 ± 4.1 in healthy and 25.3 ± 7.2 in inflamed pulp [1]. Although the methods of morphometric analysis were similar, there is a huge diversity in the results among various studies and therefore consideration should be given as to whether this method is adequate for the examination of human premolar pulp.…”

Section: Discussioncontrasting

confidence: 52%

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Characteristics of dental pulp in human upper first premolar teeth based on immunohistochemical and morphometric examinations

Tomaszewska

B²,

Borysewicz−Lewicka³

et al. 2013

Folia Histochem Cytobiol.

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Teeth extracted for orthodontic reasons are commonly considered as healthy. Therefore, it is possible to examine structure of the dental pulp can be fully recognized and how it is affected by malocclusion. The aim of the study was to evaluate by immunohistochemistry (IHC) and morphometry dental pulp in human upper first premolar teeth extracted for orthodontic reasons. The material comprised 36 teeth of 20 patients in the age range 16-26 years. By the use of IHC markers the presence of immunocompetent cells (CD20, CD45RO, and CD68), blood vessels (CD31) and nerves (PGP9.5) were examined in the pulp. Inflammatory infiltrates and tissue atrophy were observed in 24 and 10 teeth, respectively. Strong positive correlation between the width of the odontoblastic layer, the number of rows of odontoblast nuclei and the increase of MVA (microvessel area) in the pulp of atrophic teeth was found. The cellular infiltrations found in H&E-stained sections were identified by IHC as memory T cells (CD45RO+) and B lymphocytes (CD20+) with macrophages (CD68+) present at the periphery. The CD20 antigen was intensively expressed in 13 teeth, CD45RO in 33 teeth, and CD68 in 20 teeth. Thus, despite the lack of any clinical signs of pulp disease many teeth extracted for orthodontic reasons show focal pulp inflammation and atrophy which probably results from the malocclusion stress accompanying teeth crowding.

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Section: Discussioncontrasting

confidence: 52%

“…Dental pulp is a loose connective tissue of ectomesenchymal origin, resembling immature gelatinous tissue, and is enclosed in a rigid framework made of hard dental structures [1]. In the direction from dentin to tooth centre, pulp has four microscopic zones: odontoblast layer, cell-poor zone, cell-rich zone and pulp proper [2].…”

Section: Introductionmentioning

confidence: 99%

Characteristics of dental pulp in human upper first premolar teeth based on immunohistochemical and morphometric examinations

Tomaszewska

B²,

Borysewicz−Lewicka³

et al. 2013

Folia Histochem Cytobiol.

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“…Pimenta et al [32] histologically evaluated CD31 expression in inflamed and sound pulp tissue of permanent teeth referred for serial extraction. CD31 expression was greater in the inflamed tissue when associated with the expression of the lymphatic marker VEGF-3.…”

Section: Discussionmentioning

confidence: 99%

CD31 expression in human primary teeth treated with photobiomodulation therapy

et al. 2018

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Purpose This study aimed to evaluate the effects of photobiomodulation (PBM) on CD31 expression in pulp tissue repair of human primary teeth. Materials and methods Fifteen mandibular primary molars were divided into the following groups: GI-calcium hydroxide (CH), GII-PBM + CH, and GIII-PBM + zinc oxide/eugenol (ZOE). In the GII and GII groups, an indium-gallium-aluminum phosphide (InGaAlP) diode laser was used for the irradiation through 320-μm diameter optical fiber in contact with pulp tissue. The red laser diode parameters were set at 660-nm wavelength, 10-mW power output, 2.5-J/cm 2 energy density, 50-60-Hz frequency, output beam area of 0.04 cm 2 , and irradiation time of 10 s in continuous mode. After pulpotomy treatment, clinical and radiographic follow-ups were accomplished until the teeth achieved regular exfoliation period. Teeth were extracted for histological analysis and immunolocalization of CD31. Histopathological statistical analyses were performed by Kruskal-Wallis followed by Dunn test to determine statistically significant differences (p < 0.05). Results Although GII showed no inflammation or discreet inflammatory cells, the comparison among the groups using a score system revealed no statistically significant differences regarding inflammation and the amount of blood vessels. The immunohistochemistry analysis revealed positive CD31 expression in the blood vessels of all the studied groups. Immunostaining was observed in large blood vessels associated with inflammatory cells on GI and GIII, whereas GII showed discreet immunostaining predominantly in small blood vessels scattered throughout the pulp tissue with none or rare inflammatory cells. Conclusion The CD31 expression was greater in the group PBM when associated with calcium hydroxide. Photobiomodulation therapy followed by calcium hydroxide helped in repairing and seemed to regulate angiogenesis and leukocyte migration processes in the remaining pulp tissue after primary teeth pulpotomy.

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“…They are RTK and are present in three forms: fms-like tyrosine-kinase-1 (Flt-1) or VEGFR-1, fetal liver kinase-1 (Flk-1), kinase domain region (KDR) or VEGFR-2, and fms-like tyrosinekinase-4 (Flt-4) or VEGFR-3 (34). They also interact with a coreceptor family, the neuropilins, which present the VEGF to its receptor, increasing the effectiveness of the signal transduction cascade (35,36).…”

Section: Vegf and Its Receptorsmentioning

confidence: 99%