Lymphatic Migration of Immune Cells

Hampton, Henry R.; Chtanova, Tatyana

doi:10.3389/fimmu.2019.01168

Cited by 154 publications

(126 citation statements)

References 134 publications

Supporting

Mentioning

126

Contrasting

Order By: Relevance

“…Adaptive immune responses rely on a rapid, spatiotemporally concerted migration of BMDCs to the lymph nodes or to the site of inflammation [14]. Although many parts of these process are well understood, little is known about the detailed molecular mechanisms underlying the actual migration and passage through complex tissue settings and highly confined spaces.…”

Section: Resultsmentioning

confidence: 99%

Vimentin provides the mechanical resilience required for amoeboid migration and protection of the nucleus

Stankevicins

Urbanska

Flormann

et al. 2019

Preprint

View full text Add to dashboard Cite

statement (short abstract 40 words):Vimentinin joint action with actinmediates the mechanical stiffness of cells required for amoeboid cell migration through confined spaces and protects the nucleus from DNA damage. AbstractDendritic cells use amoeboid migration through constricted passages to reach the lymph nodes, and this homing function is crucial for immune responses. Amoeboid migration requires mechanical resilience, however, the underlying molecular mechanisms for this type of migration remain unknown. Because vimentin intermediate filaments (IFs) and microfilaments regulate adhesion-dependent migration in a bidirectional manner, we analyzed if they exert a similar control on amoeboid migration. Vimentin was required for cellular resilience, via a joint interaction between vimentin IFs and F-actin. Reduced actin mobility in the cell cortex of vimentin-reduced cells indicated that vimentin promotes Factin subunit exchange and dynamics. These mechano-dynamic alterations in vimentindeficient dendritic cells impaired amoeboid migration in confined environments in vitro and blocked lymph node homing in mouse experiments in vivo. Correct nuclear positioning is important in confined amoeboid migration both to minimize resistance and to avoid DNA damage. Vimentin-deficiency also led to DNA double strand breaks in the compressed dendritic cells, pointing to a role of vimentin in nuclear positioning. Together, these observations show that vimentin IF-microfilament interactions provide both the specific mechano-dynamics required for dendritic cell migration and the protection the genome needs in compressed spaces.

show abstract

Section: Resultsmentioning

confidence: 99%

Vimentin provides the mechanical resilience required for amoeboid migration and protection of the nucleus

Stankevicins

Urbanska

Flormann

et al. 2019

Preprint

View full text Add to dashboard Cite

show abstract

“…Memory T cells also enter initial lymphatics [25]. Tissue-resident memory cells lack CCR7, but migrating memory T cells express CCR7 and have the capacity to enter initial LVs, following the CCL21 gradient guidance [32]. T cell entry into afferent lymphatics is regulated by the bioactive lipid sphingosine-1-phosphate (S1P), which activates specific S1P receptors on the surface of T lymphocytes to direct migration [33].…”

Section: Lvs Serve As a Bridge Between Innate And Adaptive Immunitymentioning

confidence: 99%

Lymphatic Vessels Enhancing Adaptive Immunity Deteriorates Renal Inflammation and Renal Fibrosis

Pei

Zeng

et al. 2020

Kidney Dis

View full text Add to dashboard Cite

Background: Lymphatic vessels transport lymph away from microvascular beds into the cardiovascular system. The basic function of the lymphatic system include absorption of wa ter and macromolecules in the interstitial fluid, which plays an important role in maintaining osmotic balance of the body. Recent studies have shown that lymphangiogenesis is associated with tumor metabolism, injury repair, and chron ic inflammation, and deteriorates disease progression via immune cell trafficking. Summary: Renal interstitial lymph angiogenesis is found in patients with chronic kidney dis ease and a series of animal models of renal fibrosis. Lymphat ic vessels transfer antigen and antigenpresenting cells from peripheral tissues to lymph nodes, which initiates adaptive immunity and in turn deteriorates renal inflammation and renal fibrosis, even in nonautoimmune renal diseases. Key Messages: This review summarizes the latest findings on how lymphatics participate in the progression of chronic kid ney disease. This discussion will serve to highlight the role of adaptive immunity in noninfectious and nonautoimmune nephropathy, in order to provide new ideas and methods for prevention and treatment of kidney diseases.

show abstract

“…In our study, we showed that the PDT protocol was associated with a larger upregulation of CCR7 than that induced by LPS (however, this is known as a potent inducer of the migratory capacity of DC [31]). The high surface expression of CCR7 is associated with a high migratory responsiveness to the lymphatic-produced chemokines CCL19 and CCL21 [32][33][34]. Remarkably, we could document the increased motility of DCs in the minutes following i.p.…”

Section: Discussionmentioning

confidence: 54%

Photodynamic Therapy-Based Dendritic Cell Vaccination Suited to Treat Peritoneal Mesothelioma

Trempolec

Doix

Degavre

et al. 2020

Cancers

View full text Add to dashboard Cite

The potential of dendritic cell (DC)-based immunotherapy to treat cancer is, nowadays, well documented. Still, the clinical success of immune checkpoint inhibitors has dampened the interest in anticancer DC vaccination. For highly life-threatening tumors that are regarded as nonimmunogenic, such as mesothelioma, however, T helper 1 immunity-biased DC-based immunotherapy could still represent an attractive strategy. In this study, we took advantage of photodynamic therapy (PDT) to induce immunogenic cell death to generate mesothelioma cell lysates for DC priming and evaluated such a vaccine to treat peritoneal mesothelioma. We found that the white light in vitro activation of the photosensitizer OR141 led to mesothelioma cell death, together with the release of bona fide danger signals that promote DC maturation. The administration of a PDT-based DC vaccine to mice bearing peritoneal mesothelioma led to highly significant survival when compared with sham or control animals treated with anti-CTLA4 antibodies. This was further supported by a strong CD8+ and CD4+ T cell response, characterized by an increased proliferation, cytotoxic activities and the expression of activation markers, including interferon gamma (IFNγ). Moreover, the PDT-based DC vaccine led to a significant increase in IFNγ+ T cells infiltered within mesothelioma, as determined by flow cytometry and immunohistochemistry. Finally, in vivo tracking of intraperitoneally administered DCs led us to document rapid chemotaxis towards tumor-occupied lymphatics (vs. lipopolysaccharide (LPS)-treated DC). DCs pulsed with PDT-killed mesothelioma cells also exhibited a significant increase in CCR7 receptors, together with an intrinsic capacity to migrate towards the lymph nodes. Altogether, these results indicate that PDT-based DC vaccination is particularly suited to induce a potent immune response against peritoneal mesothelioma.

show abstract

Lymphatic Migration of Immune Cells

Cited by 154 publications

References 134 publications

Vimentin provides the mechanical resilience required for amoeboid migration and protection of the nucleus

Vimentin provides the mechanical resilience required for amoeboid migration and protection of the nucleus

Lymphatic Vessels Enhancing Adaptive Immunity Deteriorates Renal Inflammation and Renal Fibrosis

Photodynamic Therapy-Based Dendritic Cell Vaccination Suited to Treat Peritoneal Mesothelioma

Contact Info

Product

Resources

About