Lymphatic vessel invasion detected by monoclonal antibody D2-40 as a predictor of lymph node metastasis in T1 colorectal cancer

Imamura, Masayuki; Ota, Masayuki; Saito, Shôichi; Kinugasa, Yusuke; Akamoto, Shintaro; Ito, Ichiro

doi:10.1007/s00384-009-0699-x

Cited by 43 publications

(35 citation statements)

References 26 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…This is in accordance with studies investigating a risk factor predictive of nodal involvement in colorectal, vulvar and esophageal cancer [11,21,26]. Our observations indicate that the main source of lymph node metastasis may be delivered from the lymphatic invasion in esophageal cancer.…”

Section: Discussionsupporting

confidence: 92%

See 1 more Smart Citation

Lymphatic vessel invasion detected by the endothelial lymphatic marker D2-40 (podoplanin) is predictive of regional lymph node status and an independent prognostic factor in patients with resected esophageal cancer

Kozłowski¹,

Naumnik²,

Nikliński³

et al. 2011

Folia Histochem Cytobiol.

View full text Add to dashboard Cite

Abstract:The discovery of markers to lymphatic endothelial cells and the development of novel antibodies to these markers have brought increasing attention to the lymphatics and progress in the understanding of lymphangiogenesis and cancer metastasis. In this study, we investigate the presence of lymphatic vessel invasion (LVI) detected by D2-40 immunohistochemical staining in resected esophageal cancer and correlated with clinicopathologic data and patient survival. Sixty nine patients, who had a primary resection of esophageal cancer, were analyzed by univariate and multivariate logistic regression, and univariate and multivariate survival analysis. The total rate of LVI was 72% (50/69). Positive LVI was significantly correlated with lymph node metastasis (p < 0.001), tumor size (p < 0.001), histological grading (p = 0.017), tumor depth (p = 0.001), and stage (p < 0.001). Multivariate logistic analysis identified LVI (p = 0.036) as a predictor of regional lymph node metastasis. On univariate survival analysis, patients with LVI had a significantly shorter disease-free survival, cancer-specific survival and overall survival. Multivariate analysis proved that LVI diagnosed by D2-40 is an independent prognostic factor of both disease-free survival (p = 0.04) and overall survival (p = 0.032) in resected esophageal cancer. These results show that LVI assessment identifies patients at high risk for regional lymph node metastasis and that LVI is an independent prognostic factor in patients with esophageal cancer. (Folia Histochemica et Cytobiologica 2011; Vol. 49, No. 1, pp. 90-97) Key words: esophageal cancer, lymphatic vessel invasion, podoplanin, D2-40, lymph node metastasis, prognosis ageal cancer. More than 65% of patients with localized esophageal cancers and negative resection margin have a positive lymph node at the time of surgery [1]. However, despite complete tumor resection and extensive lymphadenectomy, systemic and local recurrence is common [2] and the five-year survival rate is 15-39% [3].The discovery over the last decade of markers of lymphatic endothelial cells like podoplanin (D2-40) [4], LYVE-1 [5] and Prox 1 [6], and the development of novel antibodies to these markers, have generated

show abstract

Section: Discussionsupporting

confidence: 92%

“…Lymphatic vessel invasion (LVI) is known to be an independent prognostic factor in patients with gastric and colorectal cancer [10,11]. It is also a predictor of lymph node metastasis and indicates a poor prognosis, even in node-negative patients with cervical and breast cancer [12,13].…”

Section: Introductionmentioning

confidence: 99%

Lymphatic vessel invasion detected by the endothelial lymphatic marker D2-40 (podoplanin) is predictive of regional lymph node status and an independent prognostic factor in patients with resected esophageal cancer

Kozłowski¹,

Naumnik²,

Nikliński³

et al. 2011

Folia Histochem Cytobiol.

View full text Add to dashboard Cite

show abstract

“…D2-40 (mouse monoclonal; DAKO Cytomation, Glostrup, Denmark) was used to identify the lymphatic vessels. Especially, D2-40 is useful for distinguishing the lymphatic vessels from blood vessels (Ishii et al 2009). The evaluation of tumor budding was performed by two observers who were blind to the clinical data.…”

Section: Evaluation Of Tumor Budding In Tissue Samplesmentioning

confidence: 99%

“…Possible risk factors for lymph node metastasis in submucosal invasive colorectal carcinoma include the depth of submucosal invasion, poorly differentiated histology, lymphatic vessel invasion, expression of vascular endothelial growth factor-A and -C, and expression of β-catenin (Takayama et al 1998;Maeda et al 2003;Kojima et al 2005;Walgenbach-Bruenagel et al 2006;Liang et al 2006;Saad et al 2006;Kaneko et al 2007a;Ishii et al 2009). Several studies have reported that tumor budding is a histological risk factor for lymph node metastasis of colorectal carcinoma (Hori et al 2005;Park et al 2005;Masaki et al 2006;Kaneko et al 2007b).…”

mentioning

confidence: 99%

Prognostic and Diagnostic Significance of Tumor Budding Associated with β-Catenin Expression in Submucosal Invasive Colorectal Carcinoma

Umemura

Takagi

Shimada

et al. 2013

Tohoku J. Exp. Med.

View full text Add to dashboard Cite

Endoscopic resection has become a major curative treatment for early colorectal carcinoma without lymph node metastasis. However, lymph node metastasis, a poor prognostic factor in colorectal carcinoma, occurs in about 10% of the patients with submucosal invasive colorectal carcinoma. Therefore, it is important to identify a high-risk factor for lymph node metastasis in submucosal invasive colorectal carcinoma. This study was designed to identify the relationship between tumor budding with β-catenin expression and lymph node metastasis in submucosal invasive colorectal carcinoma. We investigated the immunohistochemistry of tumor budding in the 142 patients who underwent surgical resection for submucosal invasive colorectal carcinomas between 1984 and 1999 and the expression pattern of β-catenin in budding tumor cells. Accordingly, all the patients were followed up for at least 10 years or until death. Among the 142 patients, lymph node metastasis was detected in 14 patients (9.9%). Univariate analysis showed that tumor budding with ≥ 5 tumor cells or cell clusters with expression of β-catenin in the nucleus was significantly associated with lymph node metastasis (P = 0.005). In contrast, tumor budding detected by hematoxylin and eosin staining was not associated with lymph node metastasis. Multivariate logistic regression analysis showed that tumor budding with ≥ 5 tumor cells or cell clusters with expression of β-catenin in the nucleus was a significant risk factor for lymph node metastasis (odds ratio, 7.124; 95% confidence interval, 1.407-36.062). Thus, tumor budding associated with β-catenin expression is a risk factor for lymph node metastasis in submucosal invasive colorectal carcinoma.

show abstract

“…Since cancer cells metastasize to regional lymph nodes through lymphatic vessels and to liver through venous vessels, lymphatic and venous vessel tumor invasion is the histopathological risk factor for colorectal carcinoma [6]. It is not easy to definitely identify and evaluate lymphatic vessel invasion by routine H. and E. sections, and immunocytochemical identification for the lymphatic vessel is a currently available tool for identifying lymphatic vessels [7]. There is an agreement on the evidence that more lymphatic and venous vessels are present at the margins of colonic carcinoma, suggesting that colonic carcinoma spreads to regional lymph nodes through lymphatic vessels [1]- [5] and to liver by hematogenous spread [8] [9].…”

Section: Introductionmentioning

confidence: 99%

Cancer-Associated Lymphatic and Venous Vessels in Colonic Carcinomas

Tomita¹

2014

OJPathology

View full text Add to dashboard Cite

Objective: Colonic carcinomas spread to regional lymph nodes and liver. There are cancer-associated lymphatic and venous vessels at the margin of colonic carcinomas, which facilitate spreading carcinoma through lymphatic and venous vessels. This study aimed to examine cancer-associated lymphatic and venous vessels in TNM T1 to T3 carcinomas using lymphatic vessel hyaluronan receptor for lymphatic vessels and von Willebrand factor for venous vessels by immunocytochemical staining. Materials and Methods: A total of 40 cases of moderately differentiated colonic carcinoma were studied using routinely formalin-fixed and paraffin-embedded sections. The cases consisted of 10 cases of TNM T1, 15 cases each of T2 and T3 cases. Immunocytochemical staining was performed using goat antihuman LYVE-1for lymphatic vessels and rabbit antihuman von Willebrand factor for venous vessels. Results: In TNM T1 carcinoma, increased, irregular and narrow lymphatic and venous vessels were present in the adjacent normal mucosa to the carcinoma, some of which penetrated cancerous lesion. There were no tumor emboli in lymphatic and venous vessels. In TNM T2 carcinoma, there were few lymphatic and venous vessels in midst of the carcinoma whereas numerous small lymphatic and venous vessels were present within muscle layers adjacent to the invading carcinoma. Extramural tumor embolus was present in submucosa in one case. In TNM T3 carcinoma, cancer has invaded through the muscle layers where dilated lymphatic and venous vessels were present adjacent to cancerous nests. Tumor emboli were identified in two cases by immunocytochemical staining. Conclusion: The current study showed cancer-associated lymphatic and venous vessels at the interface in TNM T1 carcinoma to dilated intramuscular lymphatic and venous vessels adjacent to invading cancerous nests in TNM T3 carcinoma, and supports cancerous cells spread via lymphatic and venous vessels through muscle layers to subserosa as supported by tumor emboli in the lymphovascular system. T. Tomita 102

show abstract

Lymphatic vessel invasion detected by monoclonal antibody D2-40 as a predictor of lymph node metastasis in T1 colorectal cancer

Abstract: LVI detected by D2-40 is important for the prediction of lymph node metastasis.

Cited by 43 publications

References 26 publications

Lymphatic vessel invasion detected by the endothelial lymphatic marker D2-40 (podoplanin) is predictive of regional lymph node status and an independent prognostic factor in patients with resected esophageal cancer

Lymphatic vessel invasion detected by the endothelial lymphatic marker D2-40 (podoplanin) is predictive of regional lymph node status and an independent prognostic factor in patients with resected esophageal cancer

Prognostic and Diagnostic Significance of Tumor Budding Associated with β-Catenin Expression in Submucosal Invasive Colorectal Carcinoma

Cancer-Associated Lymphatic and Venous Vessels in Colonic Carcinomas

Contact Info

Product

Resources

About