Lymphocyte electrophoresis as an indicator of modulation of concanavalin A-induced suppressor T-cells in vivo by anti-cancer drugs

Autoimmune mice carrying the lprcg/lprcg(lprcg),lpr/lpr(lpr),gld/gld(gld) and Yaa genes exhibit massive lymphoproliferation and a systemic lupus erythematosus-like syndrome. The surface markers of abnormally expanded lymphocytes used were Thy-1+, CD4-CD8- (double negative, DN) and CD45+ for lprcg, lpr, gld and (lprcg X gld) hybrid (F1-lprcg-gld) mice, and Ig+ for Yaa mice. To characterize the cell surface properties and differentiation pathway of lymphocytes in autoimmune mice, the cell electrophoretic mobility (EPM) was determined for the lymph node (LN), spleen and thymus cells. The EPM of lymphocytes derived from swollen LN was of the T cell type in lprcg, lpr, gld and F1-lprcg-gld mice, but of the B cell type in Yaa mice, indicating that the EPM of abnormally proliferated lymphocytes in autoimmune mice reflects their origin, and that the surface properties detected as a net negative charge were the same in abnormal and normal lymphocytes. The electrophoretic behavior of whole thymocytes was also the same in autoimmune and normal mice. The DN, and CD4+CD8- and CD4-CD8+ (single positive, SP) thymocytes from normal mice exhibited high EPM, while CD4+CD8+ (double positive, DP) thymocytes exhibited low EPM. According to the recent concept of intrathymic T cell differentiation (Schwartz, R. H., Cell. 1989, 57, 1073-1081), it is suggested that EPM of thymocytes may change with maturation in the following manner: DN thymocytes with high EPM----DP thymocytes with low EPM----SP thymocytes and autoimmune DN T cells with high EPM.

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Cell electrophoretic characterization of abnormally expanded lymphocytes in autoimmune lpr^cg, lpr, gld and Yaa mice, and of thymocyte subsets

Shimizu

Sekine²,

Matsubara

et al. 1992

Electrophoresis

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Linkage Between Monokine Production and Regulation of the Negative Surface Charge Density of Human Monocytes

Bauer

Stünkel

Kachel

1992

Immunological Investigations

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The regulation of the negative surface charge density of human monocytes was investigated with the help of the synthetic glycolipid analogue BAY R 1005. This compound is incorporated into the outer membrane of isolated monocytes during 24 hours of incubation. After this time the electrophoretic mobility (EM) of monocytes is unchanged at 0.95 x 10(-4) (cm2 V-1 s-1) and remains unchanged even under conditions where non-treated monocytes increase their EM up to 1.1 x 10(-4) (cm2 V-1 s-1). In addition BAY R 1005 stops differentiation of monocytes to macrophages, it triggers monokine production and abolishes monocyte suppressor activity and spreading capability. The results show that BAY R 1005 affects intracellular features. In connection with earlier investigations of the regulation of the negative surface charge density of human monocytes (1,2) the study suggests that monokine production and maintenance of the EM of monocytes are linked.

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Lymphocyte electrophoresis as an indicator of modulation of concanavalin A-induced suppressor T-cells in vivo by anti-cancer drugs

Cited by 2 publications

References 19 publications

Cell electrophoretic characterization of abnormally expanded lymphocytes in autoimmune lpr^cg, lpr, gld and Yaa mice, and of thymocyte subsets

Cell electrophoretic characterization of abnormally expanded lymphocytes in autoimmune lpr^cg, lpr, gld and Yaa mice, and of thymocyte subsets

Linkage Between Monokine Production and Regulation of the Negative Surface Charge Density of Human Monocytes

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Lymphocyte electrophoresis as an indicator of modulation of concanavalin A-induced suppressor T-cells in vivo by anti-cancer drugs

Cited by 2 publications

References 19 publications

Cell electrophoretic characterization of abnormally expanded lymphocytes in autoimmune lprcg, lpr, gld and Yaa mice, and of thymocyte subsets

Cell electrophoretic characterization of abnormally expanded lymphocytes in autoimmune lprcg, lpr, gld and Yaa mice, and of thymocyte subsets

Linkage Between Monokine Production and Regulation of the Negative Surface Charge Density of Human Monocytes

Contact Info

Product

Resources

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Cell electrophoretic characterization of abnormally expanded lymphocytes in autoimmune lpr^cg, lpr, gld and Yaa mice, and of thymocyte subsets

Cell electrophoretic characterization of abnormally expanded lymphocytes in autoimmune lpr^cg, lpr, gld and Yaa mice, and of thymocyte subsets