Lymphocyte proliferation stimulated by activated human macrophages treated with Canova

Burbano, Rommel Rodrı́guez; Leal, Mariana Ferreira; Costa, Joana Borges da; Bahia, Marcelo de Olivieira; Lima, Patrícia Danielle Lima de; Khayat, André Salim; Seligman, Igor Chamon; Assumpção, Paulo Pimentel de; Buchi, Dorly de Freitas; Smith, Marı́lia de Arruda Cardoso

doi:10.1016/j.homp.2008.11.011

Cited by 21 publications

(18 citation statements)

References 8 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Our group also previously observed that CA induces macrophage activation and indirectly leads to lymphocyte proliferation in vitro [1] . CA activated macrophages present an increase in the intracellular activity of the NAD(P)H oxidase and Nitric Oxide Sintetase (NOS) enzymes, therefore stimulating the production of reactive oxygen species (ROS) and nitric oxide, which might induce gene expression and/or protein modification that are likely to be an alternative signaling pathway to activate other macrophages, favoring the immune response [9] .…”

Section: Discussionsupporting

confidence: 58%

See 1 more Smart Citation

Evaluation of the Biochemical, Hematological and Genotoxic Parameters of Cebus Apella Treated with N-Methyl-Nitrosurea (NMU) Followed by Treatment with a Complex Homeopathic Compound (Canova)

Burbano¹

2016

JBT

View full text Add to dashboard Cite

Canova is a homeopathic compound that presents antimutagenic and anticancer properties. N-methylnitrosourea is a carcinogenic agent that causes alterations on DNA. This study was conducted to evaluate the antimutagenic effects of Canova on Cebus apella non-human primates treated with N-methyl-nitrosourea. Six animals were randomly assigned in two groups, one composed by two animals, who received only N-methyl-nitrosourea (NMU group) and another one consisting on four animals treated with N-methyl-nitrosourea followed by Canova treatment (CA group). Body weight, biochemical and hematological analysis were performed. Micronuclei test and Comet assay were carried out to evaluate genotoxicity. N-methyl-nitrosourea treatment led to an overall reduction in the hematologic cell count and the administration of Canova limited the adverse effects in the hematologic system on CA group. Canova also reduced micronuclei frequency and the DNA damage index. It suggests that Canova may have a role as an immune response modulator by inducing leukocyte proliferation.

show abstract

Section: Discussionsupporting

confidence: 58%

“…CA activates macrophages both in vivo and in vitro and indirectly induces lymphocyte proliferation [1] . Experiments in vivo and in vitro point out that treatment with CA can cause tumor size reduction and regression in animals, and can also recover cells viability after they were damaged [2] .…”

Section: Introductionmentioning

confidence: 99%

Evaluation of the Biochemical, Hematological and Genotoxic Parameters of Cebus Apella Treated with N-Methyl-Nitrosurea (NMU) Followed by Treatment with a Complex Homeopathic Compound (Canova)

Burbano¹

2016

JBT

View full text Add to dashboard Cite

show abstract

“…It is a complex homeopathic immunomodulator indicated for patients whose immune system is depressed. Canova activates macrophages both in vivo and in vitro and indirectly induces lymphocyte proliferation [6]. Since innate and adaptive immune responses play a role in tumor surveillance and clearance [7], enhancing the ability to trigger a specific immunologic response against malignant cells is an important anticancer approach.…”

Section: Introductionmentioning

confidence: 99%

Experimental Gastric Carcinogenesis in Cebus apella Nonhuman Primates

et al. 2011

Self Cite

View full text Add to dashboard Cite

The evolution of gastric carcinogenesis remains largely unknown. We established two gastric carcinogenesis models in New-World nonhuman primates. In the first model, ACP03 gastric cancer cell line was inoculated in 18 animals. In the second model, we treated 6 animals with N-methyl-nitrosourea (MNU). Animals with gastric cancer were also treated with Canova immunomodulator. Clinical, hematologic, and biochemical, including C-reactive protein, folic acid, and homocysteine, analyses were performed in this study. MYC expression and copy number was also evaluated. We observed that all animals inoculated with ACP03 developed gastric cancer on the 9th day though on the 14th day presented total tumor remission. In the second model, all animals developed pre-neoplastic lesions and five died of drug intoxication before the development of cancer. The last surviving MNU-treated animal developed intestinal-type gastric adenocarcinoma observed by endoscopy on the 940th day. The level of C-reactive protein level and homocysteine concentration increased while the level of folic acid decreased with the presence of tumors in ACP03-inoculated animals and MNU treatment. ACP03 inoculation also led to anemia and leukocytosis. The hematologic and biochemical results corroborate those observed in patients with gastric cancer, supporting that our in vivo models are potentially useful to study this neoplasia. In cell line inoculated animals, we detected MYC immunoreactivity, mRNA overexpression, and amplification, as previously observed in vitro. In MNU-treated animals, mRNA expression and MYC copy number increased during the sequential steps of intestinal-type gastric carcinogenesis and immunoreactivity was only observed in intestinal metaplasia and gastric cancer. Thus, MYC deregulation supports the gastric carcinogenesis process. Canova immunomodulator restored several hematologic measurements and therefore, can be applied during/after chemotherapy to increase the tolerability and duration of anticancer treatments.

show abstract

“…159,247–249 Adaptive changes in allostasis initiated by low dose nanoparticles during homeopathic treatment could help restore more normal balance between pro- and anti-inflammatory cytokines, as suggested by previous studies. 66–69,163,182,185,250–252 The result would be improvements of both psychological and physical well being across a wide range of conditions, as is claimed in hundreds of anecdotal case reports from homeopaths and supported by a number of observational trials. 253,254 …”

Section: The Net Clinical Outcome Of the Simillimum Remedy Interactiomentioning

confidence: 91%

Testing the nanoparticle-allostatic cross-adaptation-sensitization model for homeopathic remedy effects

2013

View full text Add to dashboard Cite

Key concepts of the Nanoparticle-Allostatic Cross-Adaptation-Sensitization (NPCAS) Model for the action of homeopathic remedies in living systems include source nanoparticles as low level environmental stressors, heterotypic hormesis, cross-adaptation, allostasis (stress response network), time-dependent sensitization with endogenous amplification and bidirectional change, and self-organizing complex adaptive systems. The model accommodates the requirement for measurable physical agents in the remedy (source nanoparticles and/or source adsorbed to silica nanoparticles). Hormetic adaptive responses in the organism, triggered by nanoparticles; bipolar, metaplastic change, dependent on the history of the organism. Clinical matching of the patient’s symptom picture, including modalities, to the symptom pattern that the source material can cause (cross-adaptation and cross-sensitization). Evidence for nanoparticle-related quantum macro-entanglement in homeopathic pathogenetic trials. This paper examines research implications of the model, discussing the following hypotheses: Variability in nanoparticle size, morphology, and aggregation affects remedy properties and reproducibility of findings. Homeopathic remedies modulate adaptive allostatic responses, with multiple dynamic short- and long-term effects. Simillimum remedy nanoparticles, as novel mild stressors corresponding to the organism’s dysfunction initiate time-dependent cross-sensitization, reversing the direction of dysfunctional reactivity to environmental stressors. The NPCAS model suggests a way forward for systematic research on homeopathy. The central proposition is that homeopathic treatment is a form of nanomedicine acting by modulation of endogenous adaptation and metaplastic amplification processes in the organism to enhance long-term systemic resilience and health.

show abstract

Lymphocyte proliferation stimulated by activated human macrophages treated with Canova

Cited by 21 publications

References 8 publications

Evaluation of the Biochemical, Hematological and Genotoxic Parameters of Cebus Apella Treated with N-Methyl-Nitrosurea (NMU) Followed by Treatment with a Complex Homeopathic Compound (Canova)

Evaluation of the Biochemical, Hematological and Genotoxic Parameters of Cebus Apella Treated with N-Methyl-Nitrosurea (NMU) Followed by Treatment with a Complex Homeopathic Compound (Canova)

Experimental Gastric Carcinogenesis in Cebus apella Nonhuman Primates

Testing the nanoparticle-allostatic cross-adaptation-sensitization model for homeopathic remedy effects

Contact Info

Product

Resources

About