2009
DOI: 10.2217/bmm.09.4
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Lymphocyte Proteomics of Parkinson‘s Disease Patients Reveals Cytoskeletal Protein Dysregulation and Oxidative Stress

Abstract: As a whole, peripheral blood lymphocytes are sensitive reporters of PD over inter-individual variability, and allow the identification of specific alterations that could be further exploited for diagnostic purposes.

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Cited by 30 publications
(22 citation statements)
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“…Dephosphorylated cofilin has a strong affinity for adenosine diphosphate actin and saturates F-actin, forming rods or bundles that sequester most of the cofilin and thus slow turnover of the remaining F-actin sparing ATP for other cellular processes (24,25). LIM is targeted by Parkin in PD (37), and cofilin levels are highly variant in PD lymphocytes (38). Therefore, we western blotted cofilin in protein samples from HD patient lymphocyte samples obtained from gradient density centrifugation-separated pellets from blood.…”
Section: Resultsmentioning
confidence: 99%
“…Dephosphorylated cofilin has a strong affinity for adenosine diphosphate actin and saturates F-actin, forming rods or bundles that sequester most of the cofilin and thus slow turnover of the remaining F-actin sparing ATP for other cellular processes (24,25). LIM is targeted by Parkin in PD (37), and cofilin levels are highly variant in PD lymphocytes (38). Therefore, we western blotted cofilin in protein samples from HD patient lymphocyte samples obtained from gradient density centrifugation-separated pellets from blood.…”
Section: Resultsmentioning
confidence: 99%
“…Notably, cofilin-actin inclusions have been visualized in AD brains (Minamide et al, 2000). The involvement of both actin and actin binding proteins (Lim et al, 2007;Maloney and Bamburg, 2007;Mila et al, 2009) as well as microtubule related proteins Godin et al, 2010) in neurodegeneration is becoming more evident, and cell stress has been hypothesized to be involved in these disorders (Gibson et al, 2010;Keller, 2006). Thus, being able to modulate the stress response in relation to the cytoskeleton may be a novel therapeutic approach for neurodegeneration and was the driving force behind this work.…”
Section: Discussionmentioning
confidence: 95%
“…87 Finally, use of two-dimensional gel electrophoresis and mass spectrometry has so far yielded encouraging data supporting the possibility of developing a diagnostic biomarker on the basis of a proteomic fingerprint. 88,89 The potential for defining fingerprints of combinations of lipids is now being explored. A recent investigation of lipids isolated from blood samples of healthy adults, with cognition measured in the normal range, determined a set of ten lipid species predicting development of amnestic mild cognitive impairment or AD.…”
Section: Omics Technology and Pd Biomarker Discoverymentioning
confidence: 99%