Lymphocyte Receptors and Mechanisms of in Vitro Cell‐Mediated Immune Reactions

Brondz, B. D.

doi:10.1111/j.1600-065x.1972.tb01541.x

Cited by 18 publications

(15 citation statements)

References 118 publications

(72 reference statements)

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“…These findings provide evidence to suggest that cell membrane determinants recognizable by antibodies and effector T cell receptors in the same H-2 system are not identical [ 4 4 ] . This suggestion is in accord with the concept [57] that the killer cell receptor may represent a molecular assembly whose binding efficiency may depend on accessibility and functional mobility of a particular spatial antigenic array in the target membrane which, in its turn, requires the maintenance of membrane functions in an intact TC [58].…”

supporting

confidence: 58%

Enrichment of effector T lymphocytes specific to H‐2 antigens by elution from allogeneic target cells and characterization of the eluted lymphocyte population

1975

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The technique o f elution b y pronase of murine immune lymph node cells adherent to relevant allogeneic target cells has been developed for enrichment of effector T cell population. A fraction of killer cells has been obtained possessing cytotoxic activity which exceeds that of the initial immune lymphocyte population b y a factor of 6-8 as judged by a number of lymphocytes required for 50 % cytotoxic effect (CE). T h e gain in CE is fairly reproducible in different H-2 systems and under various lymphocyte-target incubation conditions. It appears to be due t o the quantitative enrichment of the population in cells bearing receptors to the particular H-2 antigens and giving rise to specific CE rather than t o an increase in cytotoxic activity per cell resulting from treatment with the target monolayer and pronase. The eluted killer cell fraction is characterized by a twofold increase in percentage of cells killed by anti-@ antibody, a 4-5-fold increase in the number of DNA-synthesizing cells and b y an increase in medium and largc lymphocyte content. A further enrichment in effector lymphocytes is reached by depletion of B cells a t the expense of their receptors t o Fc fragment of Ig. Macrophage monolayer fixed by glutaraldehyde lost its capacity t o absorb effector lymphocytes. T h e implications of the data obtained for further studies o n cell-mediated immunity are discussed. 1 I ] and the rosetteHowever, one should not disregard the possibility that in all the above-mentioned cases passive absorption of Ig on the T cell surface is revealed rather than its true (endogenic) receptors. In fact, receptors to both F c fragment of IgM Label release upon incubation with immune and normal lymphocytes EA: Erythrocytes-antibodies 281 the possibility is not excluded that such antibodies are actually produced by small B cell contaminants present in suspension. Experimental analysis [29, 301 of these and other ambiguous points in the interpretation of such results have raised doubts whether the above cited reports really concern the endogenic T receptors. .Introduction'Meanwhile, T cell receptors responsible for interaction with target cells (TC) in the H-2 system have been shown to differ from Ig of the known classes [31-331. Such receptors are manifested in the ability of cytotoxic lymphocytes t o get selectively attached for several hours at 25-37 OC t o the naturally immunosorbent-monolayer of the relevant TC ' [34-361. To study the properties o f the effector T cells, including the nature of their receptors specific to the H-2 antigens, it seems t o be essential t o isolate these cells from the whole population where they occur in a ratio of 1-4 ' %, [ 3 7 -~3 9 ] , and to concentrate them.The present work describes a technique developed for the enrichment of the effector T lymphocyte population in cells reactive to the particular H-2 antigens. T h e ability of pronase to completely and reversibly prevent immune lymphocytes from absorption on T C monolayer with no damage of the latter described earlier [40] is t...

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confidence: 58%

Enrichment of effector T lymphocytes specific to H‐2 antigens by elution from allogeneic target cells and characterization of the eluted lymphocyte population

1975

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“…Since T killer cells only adsorb to target cells at 37°C but not at 4°C (Wilson 1965, Berke et al 1972, Golstein et al 1972, it is possible that in order for effector to target cell binding to occur, receptors on the killer cell membrane have to be rearranged. For this case, the T killer cell receptor would be an assembly of several individual receptors so that the avidity of the T killer cell would depend on the spatial arrangement of the individual receptors on its membrane (Boyse 1970, Brondz 1972, Todd & Berke 1974. The question of whether these hypothetical individual receptors are immunoglobulins or not must be answered by further experiments.…”

Section: )mentioning

confidence: 99%

Thymus Derived Killer Cells: Specificity of Function, and Antigen Recognition

Dennert

1976

Immunological Reviews

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“…The adherence and clustering of sensitized lymphoid cells on allogeneic normal target cells have been extensively studied (13,14) and have also been described for a number of tumor target cell systems (15)(16)(17)(18) …”

Section: Virusmentioning

confidence: 99%

Cellular Immunity Against Rous Sarcomas of Chickens. Preferential Reactivity Against Autochthonous Target Cells as Determined by Lymphocyte Adherence and Cytotoxicity Tests In Vitro

Wainberg¹,

Markson²,

Weiss³

et al. 1974

Proc. Natl. Acad. Sci. U.S.A.

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Spleen cells from random-bred chickens bearing Rous sarcomas were commonly more reactive against the neoplastic target cells'in autochthonous than in allogeneic interactions in vitro. This difference was observed both in cytotoxic assays (hCr release from labeled target cells) and in an immunoadherence test measuring attachment of $"Cr-labeled splenocytes to Rous sarcoma cells. Specific splenocyte reactivity was not observed with normal embryonic chicken fibroblasts, 3T3 cells, or embryonic mouse C3H fibro'blasts. The immunoadherence technique required only 2 hr to perform, and revealed a more consistent superiority of autochthonous recognition of Rous sarcoma cells than the cytotoxicity assay. Experiments in which both procedures were used simultaneously with identical cell populations yielded similar resiilts, indicating that splenocyte adherence may be a precursor of and/or concomitant to target cell damage and that individual-specific tumor antigencity may play a part in cellular immunity against Rouis sarcomas.The host-tumor relationship of chickens bearing Rous sarcomas has a number of advantages as a model pertinent to neoplasia in man. The tumor host is an outbred, nonlaboratory adapted animal, and the virus that induces the neoplastic transformation is one of a family of agents whose members are oncogenic in nature as well as in the laboratory, and active in a broad range of species in addition to the chicken (1). Cellular and humoral immunity in this system is well established both in vito and in vitro (2,3), and the chicken lends itself uniquely to the separation of lymphoid cell populations of diverse origin, and thereby to a differential definition of immunological capacity (4).The purpose of the present investigation was to compare splenocyte-target cell interaction in both autochthonous and allogeneic confrontations between Rous sarcoma cells and effector cells, by use of splenocytes sensitized during the course of the host's experience with a primary, actively growing tumor-a situation reflecting the only type of interaction that can be studied in man.This interaction was evaluated by a newly developed quantitative immunoadherence test, and by the cytotoxicity assay based on the liberation of labeled chromium from target cells.The [Medium 199 (Grand Island Biological Co., Grand Island, N.Y.) supplemented, with 5-10% inactivated calf serum (In Vitro, Jerusalem), 10% tryptose phosphate broth (Difco Laboratories), 200 units/ml of penicillin, 200 jg/ml of streptomycin, and 15-25 ml/liter of 5% NaHCOs]. The cells were washed by centrifugation, pooled, counted, and seeded.Other Cell Types. Normal chicken embryo cells (CEC) were secondary cultures (2-to 3-days-old) derived from 9-to 11-

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Lymphocyte Receptors and Mechanisms of in Vitro Cell‐Mediated Immune Reactions

Cited by 18 publications

References 118 publications

Enrichment of effector T lymphocytes specific to H‐2 antigens by elution from allogeneic target cells and characterization of the eluted lymphocyte population

Enrichment of effector T lymphocytes specific to H‐2 antigens by elution from allogeneic target cells and characterization of the eluted lymphocyte population

Thymus Derived Killer Cells: Specificity of Function, and Antigen Recognition

Cellular Immunity Against Rous Sarcomas of Chickens. Preferential Reactivity Against Autochthonous Target Cells as Determined by Lymphocyte Adherence and Cytotoxicity Tests In Vitro

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