Lymphocyte Subpopulations and Cytokine Production in Paracoccidioidomycosis Patients

Bava, Amadeo Javier; Mistchenko, Alicia; Palacios, María Fernanda; Estévez, María; Tiraboschi, Nora I.; Sen, Luisa; Negroni, Ricardo; Díez, Roberto A.

doi:10.1111/j.1348-0421.1991.tb01545.x

Cited by 51 publications

(42 citation statements)

References 14 publications

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“…Magnification, ϫ200 for figures A, B, C, F, and ϫ400 for E. the mobilization of DC into the peripheral circulation and tissues (30). We did not confirm that the ratio of CD4-CD8 was decreased in patients with PCM (31). The latter results may reflect the fact that patients studied here exhibited the chronic form of disease.…”

Section: Discussioncontrasting

confidence: 54%

Systemic and Local Characterization of Regulatory T Cells in a Chronic Fungal Infection in Humans

Cavassani¹,

Campanelli²,

Moreira³

et al. 2006

The Journal of Immunology

129

106

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The long-term persistence of pathogens in a host is a hallmark of certain infectious diseases, including schistosomiasis, leishmaniasis, and paracoccidioidomycosis (PCM). Natural regulatory T (Treg) cells are involved in control of the immune responses, including response to pathogens. Because CTLA-4 is constitutively expressed in Treg cells and it acts as a negative regulator of T cell activation in patients with PCM, here we investigated the involvement of Treg cells in the control of systemic and local immune response in patients with PCM. We found that the leukocyte subsets were similar in patients and controls, except for CD11c+CD1a+ cells. However, a higher frequency of CD4+CD25+ T cells expressing CTLA-4, glucorticoid-inducible TNFR, membrane-bound TGF-β, and forkhead-box 3 were observed in PBMC of patients. In accordance, these cells exhibited stronger suppressive activity when compared with those from controls (94.0 vs 67.5% of inhibition of allogeneic T cell proliferation). In addition, the data showed that CD4+CD25+ T cells expressing CTLA-4+, glucocorticoid-inducible TNFR positive, CD103+, CD45RO+, membrane-bound TGF-β, forkhead-box 3 positive, and the chemokines receptors CCR4 and CCR5 accumulate in the Paracoccidioides brasiliensis-induced lesions. Indeed, the secreted CCL17 and CCL22, both associated with the migration of Treg cells to peripheral tissues, were also detected in the biopsies. Moreover, the CD4+CD25+ T cell derived from lesions, most of them TGF-β+, also exhibited functional activity in vitro. Altogether, these data provide the first evidence that Treg cells play a role in controlling local and systemic immune response in patients with a fungal-induced granulomatous disease advancing our understanding about the immune regulation in human chronic diseases.

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Section: Discussioncontrasting

confidence: 54%

Systemic and Local Characterization of Regulatory T Cells in a Chronic Fungal Infection in Humans

Cavassani¹,

Campanelli²,

Moreira³

et al. 2006

The Journal of Immunology

129

106

View full text Add to dashboard Cite

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“…In T-cell subpopulations in the peripheral blood of patients with acute or chronic forms of PCM, a significant fall in the CD4/CD8 T-cell ratio was detected (2,30). Some authors observed decreased numbers of CD4 ϩ and CD8 ϩ T cells (30), whereas others reported a decrease only in the CD4 ϩ subset (2).…”

Section: Discussionmentioning

confidence: 96%

Depletion of CD8⁺T Cells In Vivo Impairs Host Defense of Mice Resistant and Susceptible to Pulmonary Paracoccidioidomycosis

et al. 2000

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“…Another study showed a decrease in CD4 ϩ lymphocyte levels in patients with fungal infections (5). An opposite trend (consisting of an increase in CD4 ϩ lymphocyte levels) was reported for patients with meningitis infection, however, whereas Staphylococcus infection caused a reduction in the proportion of CD11 ϩ and CD8 ϩ lymphocytes (2).…”

Section: Discussionmentioning

confidence: 99%

Flow Cytometry Study of Lymphocyte Subsets in Malnourished and Well-Nourished Children with Bacterial Infections

Nájera¹,

González

Toledo

et al. 2004

Clin Vaccine Immunol

102

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Protein-energy malnutrition is the primary cause of immune deficiency in children across the world. It has been related to changes in peripheral T-lymphocyte subsets. The aim of the present study was to evaluate the effects of infection and malnutrition on the proportion of peripheral-lymphocyte subsets in well-nourished non-bacterium-infected (WN), well-nourished bacterium-infected (WNI), and malnourished bacterium-infected (MNI) children by flow cytometry. A prospectively monitored cohort of 15 MNI, 12 WNI, and 17 WN children was studied. All the children were 3 years old or younger and had only bacterial infections. Results showed a significant decrease in the proportion of T CD3 ؉ (P < 0.05 for relative and P < 0.03 for absolute values), CD4 ؉ (P < 0.01 for relative and absolute values), and CD8 ؉ (P < 0.05 for relative values) lymphocyte subsets in WNI children compared to the results seen with WN children. Additionally, B lymphocytes in MNI children showed significant lower values (CD20 ؉ P < 0.02 for relative and P < 0.05 for absolute values) in relation to the results seen with WNI children. These results suggest that the decreased proportions of T-lymphocyte subsets observed in WNI children were associated with infection diseases and that the incapacity to increase the proportion of B lymphocyte was associated with malnutrition. This low proportion of B lymphocytes may be associated with the mechanisms involved in the immunodeficiency of malnourished children.Protein-energy malnutrition is a public health problem in developing countries. Around the world, each year 12 million children under 5 years old die due to the relationship between malnutrition and infectious diseases. Even if malnutrition is very extensive in developing countries, it is rarely considered one of the main mortality causes. However, recent studies indicated that malnutrition in developing countries is an associated death cause in the deaths of more than 50% of children (34). In community studies, a clear relationship between weight decrease and increase of mortality risk has been observed. Also, a solid and systematic relationship between malnutrition and a higher risk of respiratory and diarrhea infections have been detected (27). Several authors have pointed out that malnutrition is the primary cause of immunodeficiency in the world (8,12,32), and it has been related to changes in cellular immunity (8, 29) and changes in peripheral-lymphocyte subsets (mainly CD3 ϩ , CD4 ϩ , and CD8 ϩ ). Nevertheless, results of studies investigating this topic are controversial. In some studies an increase of lymphocyte proportion has been observed; meanwhile, other studies show a decrease in lymphocyte proportions (4,7,10). In a previous study Nájera et al. found no changes in lymphocyte T subsets when malnourished and well-nourished children with bacterial infections were compared (18). These controversial results may be associated with several factors, such as the methods used, the type and degree of malnutrition, and the infection type. For th...

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Lymphocyte Subpopulations and Cytokine Production in Paracoccidioidomycosis Patients

Abstract: Despite the postulated role of the immune system in the control of the infection by Paracoccidioides brasiliensis, only a few studies have addressed this point in patients.

Cited by 51 publications

References 14 publications

Systemic and Local Characterization of Regulatory T Cells in a Chronic Fungal Infection in Humans

Systemic and Local Characterization of Regulatory T Cells in a Chronic Fungal Infection in Humans

Depletion of CD8⁺T Cells In Vivo Impairs Host Defense of Mice Resistant and Susceptible to Pulmonary Paracoccidioidomycosis

Flow Cytometry Study of Lymphocyte Subsets in Malnourished and Well-Nourished Children with Bacterial Infections

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Lymphocyte Subpopulations and Cytokine Production in Paracoccidioidomycosis Patients

Abstract: Despite the postulated role of the immune system in the control of the infection by Paracoccidioides brasiliensis, only a few studies have addressed this point in patients.

Cited by 51 publications

References 14 publications

Systemic and Local Characterization of Regulatory T Cells in a Chronic Fungal Infection in Humans

Systemic and Local Characterization of Regulatory T Cells in a Chronic Fungal Infection in Humans

Depletion of CD8+T Cells In Vivo Impairs Host Defense of Mice Resistant and Susceptible to Pulmonary Paracoccidioidomycosis

Flow Cytometry Study of Lymphocyte Subsets in Malnourished and Well-Nourished Children with Bacterial Infections

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Depletion of CD8⁺T Cells In Vivo Impairs Host Defense of Mice Resistant and Susceptible to Pulmonary Paracoccidioidomycosis