Lymphocyte subpopulations in rheumatoid arthritis

Vries, E. de; Meijer, C. J. L. M.; Lafeber, G. J. M.; Cnossen, J.; Cats, A.

doi:10.1007/bf00541203

Cited by 16 publications

(3 citation statements)

References 18 publications

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“…Although the dual staining we noted may have been somewhat greater than the values given in previous reports, the levels did not differ in PB from RA patients and that from normal subjects. Additionally, the findings of this study confirm the previously reported increase in activation markers in RA SF lymphocytes, as well as the increase in CD8 + SF lymphocytes, compared with RA PB (6)(7)(8)(9)(10)(11)(12)(13)(14)28). Furthermore, we observed no significant increase in dual-stained Leu-2 + /Leu-3 + lymphocytes in either RA PB or RA SF, which is contrary to findings in a previous report (1 1).…”

Section: Discussionsupporting

confidence: 90%

Increased helper inducer and decreased suppressor inducer phenotypes in the rheumatoid joint

Lasky

Bauer

Pope

1988

Arthritis & Rheumatism

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Cells isolated from the joints of patients with rheumatoid arthritis (RA) exhibit functional immune abnormalities, such as diminished suppressor activity, depressed' response to mitogens, and enhanced immunoglobulin production. We sought to characterize the T lymphocyte subsets in the synovial fluid (SF) and peripheral blood (PB) of RA patients in an attempt to clarify the mechanism(s) responsible for these functional immune abnormalities. We used dual-immunofluorescence staining techniques with several combinations of monoclonal antibodies, including anti-4B4 and anti-2H4, which define, respectively, the helper inducer and suppressor inducer subsets of CD4+ (Leu3+ and T4+) cells. Mononuclear cells from normal PB (n = 9), RA PB (n = 6), and RA SF (n = 9) were analyzed, after staining, by flow cytometry. We observed a significant increase (P < 0.0002) in the number of cells bearing the helper inducer phenotype (CD4 + ,4B4 + ), and a significant decrease (P < 0.0002) in the number of cells bearing the suppressor inducer phenotype (CD4 + , 2H4+), in RA SF compared with the levels in PB from From the

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Section: Discussionsupporting

confidence: 90%

Increased helper inducer and decreased suppressor inducer phenotypes in the rheumatoid joint

Lasky

Bauer

Pope

1988

Arthritis & Rheumatism

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“…This increase is consistent with our findings when weakly staining CD8+, CD3non-T cells were excluded from the analysis. Our mean value for the CD4:CD8 ratio in the synovial fluid is consistent with previously reported values (5,7,9,10,18,19). In the peripheral blood, few T lymphocytes are activated in terms of DR expression (30), but CD3+ T cells in the synovial fluid express a considerable amount of DR antigens, as previously described (12)(13)(14)31,32).…”

Section: Discussionsupporting

confidence: 91%

T Cell Receptor Expression And Activation Of Synovial Lymphocyte Subsets In Patients With Rheumatoid Arthritis

Rème

Portier

Frayssinoux

et al. 1990

Arthritis & Rheumatism

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Two-color flow cytometry analysis of peripheral blood and synovial lymphocytes from rheumatoid arthritis patients was performed using monoclonal antibodies directed against T cell subsets, T cell activation markers, and T cell receptors. The results showed an abnormally high percentage (>15%) of CD3+, CD4-, and CDS-T cells expressing a specific receptor containing a y chain. Phenotypic analysis of lymphocytes infiltrating both knee joints of individual rheumatoid arthritis patients revealed very similar subset distribution and activation levels, despite strong differences in the clinical status between the 2 sites.

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“…tissue sections. Rheumatoid synovial tissue is infiltrated mainly by CY)A* T cells [24][25][26]. but while CD4' T eells accumulate around blood vessels.…”

Section: Discussionmentioning

confidence: 99%

T‐Cell Receptor β‐Chain Gene Rearrangements of T‐Cell Populations Expanded from Multiple Sites of Synovial Tissue obtained from a Patient with Rheumatoid Arthritis

et al. 1992

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In this study T-cell receptor (TcR) beta-chain gene rearrangements of T-cell lines prepared from multiple sites (n = 92) of synovial tissue derived from both knees of a patient with rheumatoid arthritis were analysed. In the majority of T-cell lines, dominant TcR beta-chain gene rearrangements were detected, involving C beta 1 as well as C beta 2. The dominant rearrangement patterns of T-cell lines from different tissue fragments showed significant variability, but some of the DNA restriction fragments were shared by T-cell lines from multiple sites in both knees. The latter observation suggests that identical T-cell clones may be present at different sites in the synovial tissue and in different joints. However, since many T-cell lines yielded different rearrangement patterns, these data also indicate considerable heterogeneity of T cells in the joints. Apart from theoretical implications, this TcR heterogeneity of T cells within an individual patient also has practical consequences for studies on synovial T cells obtained by biopsy.

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Lymphocyte subpopulations in rheumatoid arthritis

Cited by 16 publications

References 18 publications

Increased helper inducer and decreased suppressor inducer phenotypes in the rheumatoid joint

Increased helper inducer and decreased suppressor inducer phenotypes in the rheumatoid joint

T Cell Receptor Expression And Activation Of Synovial Lymphocyte Subsets In Patients With Rheumatoid Arthritis

T‐Cell Receptor β‐Chain Gene Rearrangements of T‐Cell Populations Expanded from Multiple Sites of Synovial Tissue obtained from a Patient with Rheumatoid Arthritis

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