Lymphocytes infiltrating rat cardiac allografts express a limited repertoire of T cell receptor V beta genes.

Shirwan, H; Chi, Dongmei; Makowka, Leonard; Cramer, Donald V.

doi:10.4049/jimmunol.151.10.5228

Cited by 41 publications

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Shared amino acid sequences in the NDβN and Nα regions of the T cell receptors of tumor‐infiltrating lymphocytes within malignant glioma

et al. 1994

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The purpose of this study was to assess the V-(D)-J junctional region of the T cell receptor (TCR), the CDR3 region, which is responsible for glioma-specific antigen contact in alpha beta TCR-mediated recognition. We sequenced the TCR alpha and beta chains of V alpha 7, and V beta 13.1 cDNA derived from tumor-infiltrating lymphocytes (TIL) of 12 glioma patients and also the corresponding clones from the patients' peripheral blood lymphocytes (PBL). A shared V beta 13.1 DJ sequence of the CDR3 region, ND beta N, was demonstrated in 49 of 66 V beta 13.1+ clones (74.2%) from the glioma TIL, whereas only 4 of 33 clones (12.1%) were observed in the V beta 13.1+ clones from the PBL (p < 0.001). A common VDJ sequence, FCASS (V beta 13.1)-YRLPWGTSDS (ND beta N)-GELFF (J beta 2.2), was observed not only in the gliomas from each patient, but also among all the patients with a preference for V beta 13.1. In contrast, the amino acid sequences of the V beta 13.1+ PBL clones were diverse and random. Next, we sequenced subclones from other V beta subfamilies randomly selected to compare their VDJ region rearrangements (V beta 3 and V beta 5.1). In contrast to V beta 13.1, the amino acid sequences of these junctional regions were completely different in these subclones. The V-J junctional region of the alpha chain is dominated by a few clones in some patients, and no shared amino acid sequences were detected in the TCR V alpha junctional region. However, in the N alpha region of the V alpha 7-bearing TIL clones, arginine was used in 27 of 44 clones (61.4%) compared to only 3 of 12 clones from the PBL (p < 0.05). These results are consistent with the hypothesis that a clonal expansion/accumulation of glioma lineage-specific T cells occurred in vivo at the tumor site and that these T cells may be recognizing glioma-specific antigens.

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Shared amino acid sequences in the NDβN and Nα regions of the T cell receptors of tumor‐infiltrating lymphocytes within malignant glioma

et al. 1994

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T cell receptor diversity in alloreactive responses against HLA‐B27 (B*2705) is limited by multiple‐level restrictions in both α and β chains

1995

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The T cell receptors (TCR) in HLA-B27 (B*2705) alloreactivity were analyzed in cytotoxic T lymphocytes (CTL) from two individuals. Non-random usage was found in V beta, N+D beta, V alpha, and J alpha, but not in J beta segments or N alpha-regions. V beta segments from homology subgroup 4 were predominant and not associated to a particular donor or fine specificity, suggesting involvement in recognizing the HLA-B27 molecule. In contrast, preferential V alpha usage was associated with particular individuals and fine specificities, indicating distinct V beta and V alpha recruitment and contribution to allorecognition. Recurrent N+D beta motifs and J alpha segments, even from different donors, limited junctional diversity, suggesting that CDR3 usage was determined by the alloantigenic epitope independently of individuals. TCR were selected differently at various levels, as indicated by the following findings. Four clonotypes with similar fine specificity had identical beta and unrelated alpha chains. Similar alpha were associated with unrelated beta chains, and vice versa. CTL using V beta subgroup 4 did not globally show concomitant predominance of other TCR elements. V alpha 7, one of the preferred V alpha segments, was always associated with V beta subgroups other than 4. Sometimes, a TCR showed homology in elements of one chain to a second TCR or group of TCR, and to another in the other chain. These results are best explained by differential selection of TCR elements by different epitopes, providing a key to the inner structure of allospecific TCR repertoires.

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Evidence for Preferential T Cell Receptor Vβ Gene Usage and T Cell Clonal Expansion in the Synovium of BB Rats with Early-Onset Collagen-Induced Arthritis

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Endres

et al. 1998

Cellular Immunology

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Lymphocytes infiltrating rat cardiac allografts express a limited repertoire of T cell receptor V beta genes.

Cited by 41 publications

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Shared amino acid sequences in the NDβN and Nα regions of the T cell receptors of tumor‐infiltrating lymphocytes within malignant glioma

Shared amino acid sequences in the NDβN and Nα regions of the T cell receptors of tumor‐infiltrating lymphocytes within malignant glioma

T cell receptor diversity in alloreactive responses against HLA‐B27 (B*2705) is limited by multiple‐level restrictions in both α and β chains

Evidence for Preferential T Cell Receptor Vβ Gene Usage and T Cell Clonal Expansion in the Synovium of BB Rats with Early-Onset Collagen-Induced Arthritis

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