2012
DOI: 10.1100/2012/629326
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Lymphocytes Mitochondrial Physiology as Biomarker of Energy Metabolism during Fasted and Fed Conditions

Abstract: Mitochondria are central coordinators of energy metabolism, and changes of their physiology have long been associated with metabolic disorders. Thus, observations of energy dynamics in different cell types are of utmost importance. Therefore, tools with quick and easy handling are needed for consistent evaluations of such interventions. In this paper, our main hypothesis is that during different nutritional situations lymphocytes mitochondrial physiology could be associated with the metabolism of other cell ty… Show more

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Cited by 12 publications
(9 citation statements)
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“…Lymphocyte mitochondrial physiology seems to be associated with nutritional status (Briet et al, 2003) and other cell type metabolic alterations. Therefore, it might be considered an interesting biomarker of metabolic status (Cortez et al, 2012). In this context, we used PBMCs to determine both AMPK activity and SIRT-1 expression in controls vs. obese vs. Post-surgery patients.…”
Section: Discussionmentioning
confidence: 99%
“…Lymphocyte mitochondrial physiology seems to be associated with nutritional status (Briet et al, 2003) and other cell type metabolic alterations. Therefore, it might be considered an interesting biomarker of metabolic status (Cortez et al, 2012). In this context, we used PBMCs to determine both AMPK activity and SIRT-1 expression in controls vs. obese vs. Post-surgery patients.…”
Section: Discussionmentioning
confidence: 99%
“…We used high-resolution respirometry protocol according to Cortez et al, 2012 [ 31 ]. Briefly, liver fragments were mechanically macerated by using sharpened forceps and transferred into vessels with cooled Mitochondrial Respiration Medium, MIR05.…”
Section: Methodsmentioning
confidence: 99%
“…Lymphocytes make up the majority of the peripheral blood mononuclear cells. Lymphocyte energy metabolism has been used to measure defects in mitochondrial OXPHOS and to screen for OXPHOS disorders [9, 14, 15]. Altered mitochondrial OXPHOS in lymphocytes and peripheral blood mononuclear cells has been detected in patients with systematic lupus erythematosus [16], depression [17], inflammation of overweight elderly [18], septic shock [1921], Alzheimer disease [13], Huntington’s disease [22, 23], and medications and age [24].…”
Section: Introductionmentioning
confidence: 99%