Lymphocytosis and lymphadenopathy in a dog arising from two distinct lymphoid neoplasms

Abstract

A 10-year-old intact male Golden Retriever was presented to The Ohio State University Veterinary Medical Center for acute, non-painful facial swelling of the right mandibular region. On physical examination, the right mandibular swelling was found to represent marked lymphadenopathy of the submandibular lymph node. At this time, marked lymphadenopathy of the prescapular and popliteal lymph nodes was also appreciated. Abdominal lymphadenopathy with suspected splenic and hepatic involvement was detected on ultrasound, based on mass effects and echogenic changes. The CBC showed a moderate leukocytosis (38.4 × 10 9 cells/L, reference interval [RI] 4.8-13.9 × 10 9 cells/L) characterized by a moderate lymphocytosis (28.4 × 10 9 cells/L, RI 1.0-4.6 × 10 9 cells/L). A mild hyperproteinemia (7.5 g/dL, RI 5.6-7.3 g/dL) was also determined by refractometry; however, no significant abnormalities were observed on the blood chemistry panel. Review of the blood smear confirmed the lymphocytosis, which was characterized by a homogeneous population of intermediate lymphocytes (98%) with the remainder being small and

Flow Cytometry in the Diagnosis of Canine B-Cell Lymphoma

“…Finally, the multiparametric approach of FC is extremely useful also to identify rarer cases of two coexisting neoplasms, such as T-zone lymphoma and BCL (35)(36)(37). An example is shown in Figure 3, where both BCL and TZL populations are shown at diagnosis, at the end of CHOP chemotherapeutic protocol, and at relapse of BCL.…”

Flow Cytometry in the Diagnosis of Canine T-Cell Lymphoma

“…Expression of CD4 and CD8 may be variable (CD4+CD8-, CD4-CD8+, CD4-CD8-, or CD4+CD8+) without any evident correlation with outcome. Due to the very peculiar phenotype, TZL also may be easy to recognize in its earlier phases, when a mixed population of residual non-neoplastic lymphocytes is still present, in cases of concurrent lymphomas in the same node (29)(30)(31) or, again, when they are detected in non-lymphoid tissues, such as peripheral blood and bone marrow, which are infiltrated in more than 90% of cases (32). The low proliferative activity reported by TZLs is described in two studies focusing on B and TCLs and supports the low aggressiveness of the tumor (26,27).…”

Tumor staging in a Beagle dog with concomitant large B-cell lymphoma and T-cell acute lymphoblastic leukemia

“…A Golden Retriever dog has been reported to have concomitant B-cell lymphoma and T-zone lymphoma (TZL). 8 However, TZL has an indolent clinical course, with prolonged survival even in the absence of therapy, 11,17 and might have gone unnoticed in the dog until B-cell lymphoma caused clinical signs. Conversely, both LBCL and T-ALL are aggressive diseases, unlikely to remain unnoticed for long periods.…”