Lymphokine Production by PHA-Stimulated Human Lymphocytes is Enhanced by Interferon

Blomgren, Henric; Einhorn, Stefan

doi:10.1159/000232816

Cited by 17 publications

(2 citation statements)

References 14 publications

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“…Perhaps enhanced Ly-6 antigen expression mediated by IFN amplifies the capacity to T cells to respond to additional stimuli. Interestingly, earlier studies have shown that pretreatment of human T cells with IFN augments their subsequent release of lymphokines upon exposure to phytohemagglutinin [25].…”

Section: Discussionmentioning

confidence: 99%

Interferon‐α/β enhances the expression of Ly‐6 antigens on T cells in vivo and in vitro

Dumont

Coker

1986

Eur J Immunol

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Interferons (IFN) have been shown to modulate the expression of a number of cell surface antigens on macrophages and lymphocytes. Because such phenotypic alterations may be involved in the functional effects of IFN, it appears important to characterize further these alterations. In the present work, we evaluated the response to IFN of Ly-6-encoded molecules on murine T cells. Two types of Ly-6 antigens, Ly-6A and Ly-6C, normally present on minor subsets of mature T cells were studied. It was found that in vivo treatment of mice with the IFN inducer poly(I . C) or with purified IFN-alpha/beta resulted two days later in augmented expression of these antigens. Purified T cells cultured in vitro for 2 days in the presence of 5% serum from poly(I . C)-treated mice or of 10(4) units/ml IFN-alpha/beta also displayed dramatically increased (4-12-fold) amounts of Ly-6 antigens. Under the same conditions, the T cell markers Thy-1, Ly-1, Lyt-2 and MT4 were unaffected or slightly diminished while surface expression of H-2 or beta 2-microglobulin antigens was increased by only 10-36%. Therefore, poly(I . C)-induced or purified IFN interacts with resting T cells to selectively enhance Ly-6 antigen expression. This phenomenon was found to correlate functionally with increased proliferative response of the T cells, in presence of phorbol myristate acetate, to anti-Ly-6 antibodies cross-linked on their surface. Enhancement of Ly-6 antigen expression on T cells may thus play a role in IFN-mediated immunoregulation.

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Section: Discussionmentioning

confidence: 99%

Interferon‐α/β enhances the expression of Ly‐6 antigens on T cells in vivo and in vitro

Dumont

Coker

1986

Eur J Immunol

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“…(31,3 2) It can augment the cytotoxic activity of NK cells (33,34) and the production of certain cytokines. (35)(36)(37)(38)(39) However, in spite of extensive studies, the mechanism s by which the immunostimulatory actions of IFN-a occur are not fully understood.…”

Section: Introduction F Ibr O M Y Amentioning

confidence: 99%

Lymphocyte Markers and Natural Killer Cell Activity in Fibromyalgia Syndrome: Effects of Low-Dose, Sublingual Use of Human Interferon-alpha

Russell¹,

Vipraio²,

Michalek

et al. 1999

Journal of Interferon & Cytokine Research

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A clinical study was designed to utilize flow cytometric immunophenotyping and chromium release from cultured tumor target cells to characterize peripheral blood mononuclear leukocyte (PBML) subpopulations and natural killer activity in healthy normal controls (n = 18) and in patients with fibromyalgia syndrome (FMS) at baseline (n = 124) and again after 6 weeks of treatment with low-doses of orally administered human interferon-alpha (IFN-alpha). Volunteer subjects discontinued all analgesic and sedative hypnotic medications for 2 weeks prior to the baseline phlebotomy. Laboratory measures included a complete blood count; a phenotypic analysis of PBML by flow cytometry; and in vitro natural killer (NK) cell activity. After baseline blood sample collection, the FMS patients were randomized to one of four parallel treatment groups (n = 28/group) to receive sublingual IFN-alpha (15 IU, 50 IU, 150 IU), or placebo every morning for 6 weeks. The tests were repeated at week 6 to evaluate treatment effects. At baseline, FMS patients exhibited fewer lymphocytes and more CD25+ T lymphocytes than did normal controls. By week 6, the main significant and consistent change was a decrease in the HLA-DR+ CD4+ subpopulation in the 15 IU and 150 IU treatment groups. These data do not support an immunologically dysfunctional PBML phenotype among patients with FMS as has been observed in the chronic fatigue syndrome.

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Anti‐proliferative effects of interferons on Daudiburkitt Lymphoma cells: Induction of cell differentiation and loss of response to autocrine growth factors

Exley

Gordon

Nathan

et al. 1987

Intl Journal of Cancer

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Treatment of Daudi B-lymphoblastoid cells with low concentrations of either natural or recombinant human alpha-interferons inhibits cell proliferation and modulates the expression of a number of cell-surface antigens. Using a panel of monoclonal antibodies (MAbs) identifying determinants expressed at the surface of normal plasma cells, and polyclonal antibodies against surface and cytoplasmic immunoglobulin, we have found that growth inhibition is accompanied by plasmacytoid differentiation. Assays of growth stimulation of heterologous cells indicate that the culture medium from interferon-treated Daudi cells contains substantially more B-cell growth factor activity than that from control cells. However, the interferon-treated cells exhibit an impaired ability to respond to both these autocrine factors and exogenous factors produced by another Burkitt lymphoma line. These findings show that, in the case of Daudi cells, growth inhibition by interferons is closely associated with both terminal differentiation and a refractoriness to growth factors. In this system IFN-alpha may therefore be considered to be a B-cell differentiation factor, suggesting a possible basis for the anti-proliferative effects observed with certain human B-cell malignancies.

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Lymphokine Production by PHA-Stimulated Human Lymphocytes is Enhanced by Interferon

Cited by 17 publications

References 14 publications

Interferon‐α/β enhances the expression of Ly‐6 antigens on T cells in vivo and in vitro

Interferon‐α/β enhances the expression of Ly‐6 antigens on T cells in vivo and in vitro

Lymphocyte Markers and Natural Killer Cell Activity in Fibromyalgia Syndrome: Effects of Low-Dose, Sublingual Use of Human Interferon-alpha

Anti‐proliferative effects of interferons on Daudiburkitt Lymphoma cells: Induction of cell differentiation and loss of response to autocrine growth factors

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