Lymphoreticular cells in human brain tumours and in normal brain

Abstract

Summary.-The present investigation, using various rosetting assays of cell suspensions prepared by mechanical disaggregation or collagenase digestion, demonstrated lymphoreticular cells in human normal brain (cerebral cortex and cerebellum) and in malignant brain tumours. The study revealed T and B lymphocytes and their subsets (bearing receptors for Fc(IgG) and C3) in 5/14 glioma suspensions, comprising < 15% of the cell population. Between 20-60% of cells in tumour suspensions morphologically resembled macrophages and s75%0 of these cells formed strong rosettes.Lymphocytes were not found in cancer-free (putatively normal) brain. Macrophages and the smaller "microglial cells" (both phagocytic, staining with sudan black, and expressing Fc(IgG) and C3 receptors) were found in normal brain in numbers similar to those in tumour suspensions, but with less rosetting avidity. These cells may be part of an immunological defence mechanism.

Invasiveness of primary brain tumors

“…The inflammatory cells consist of different types of lymphocytes, including T and B cells, and their subsets with Fc and C3 receptors. These cells have been observed to comprise up to 15% of the total tumor cell population (20). This is in striking contrast to the cancer free, putatively normal brain.…”

“…This is in striking contrast to the cancer free, putatively normal brain. Macrophages are also regularly found (20,21). In some cases these cells can comprise up to 3/4 of the cell population in a given tumor (22).…”

Is the restricted ketogenic diet a viable alternative to the standard of care for managing malignant brain cancer?

“…GBM is heterogeneous in cellular composition consisting of tumor stem cells, mesenchymal cells, and host stromal cells (Tso et al, 2006;Ohgaki and Kleihues, 2009;Chen et al, 2010;Prestegarden et al, 2010). In addition to the neoplastic cell populations, tumor-associated macrophages/monocytes (TAM) also comprise a significant cell population in GBM sometimes equaling the number of tumor cells (Morantz et al, 1979;Phillips et al, 1982;Shinonaga et al, 1988;Nishie et al, 1999;Seyfried, 2001;Seyfried et al, 2010a). TAM indirectly contributes to tumor progression through release of pro-inflammatory and proangiogenic factors (Nishie et al, 1999;Seyfried, 2001;Lewis and Murdoch, 2005;Seyfried et al, 2010a).…”

Immune responses in malignant gliomas of brain: literature review

“…A. Stevens та співавтори [17] показали незначну інфільтрацію натуральними кілерами (НК-клітинами) в усіх 18 проаналізованих зразках гліом. Подібні результати відзначені й інши-ми дослідниками, які підтвердили наявність різного вмісту ПІЛ в злоякісних глімах [18][19][20][21][22]. В цілому, мінливість вмісту ПІЛ у гліомах, ймовірно, пов'язана з різною методологією та методами дослідження, через невідповідність результатів оцінки частоти, ступеня інфільтрації, локалізації ПІЛ у пухлинному вогнищі.…”