Lymphoreticular cells in invasive carcinoma of the uterine cervix: an immunohistological study

Abstract

In the present study the immunohistology of the cellular stromal reaction of invasive squamous cell carcinoma of the cervix is investigated. Tumor tissue from 10 patients with invasive squamous cell carcinoma of the uterine cervix (stages Ib-IIb, according to FIGO) was immunostained by the alkaline phosphatase-anti-alkaline phosphatase (APAAP) method. The monoclonal antibodies OKT3, OKT4, OKT8, TO15, Ki-M1, and Ki-M6 were applied. The cells in the stroma and the tumor foci were evaluated separately. In all cases, the overwhelming majority of lymphoreticular cells were found in the stroma and the tumor-cell complexes contained relatively low numbers of these cells. While B-lymphocytes were present only in low numbers or were virtually absent from the lymphoreticular infiltrates, cells of the mononuclear-phagocyte system were found to be another prominent constituent of the tumor's cellular stromal reaction.

Reversed CD4/CD8 ratios of tumor-infiltrating lymphocytes are correlated with the progression of human cervical carcinoma

“…[13][14][15][16][17][18][19] However, little is known about the properties of TILs in solid tumors from limited data of in situ histochemical staining of tissue sections, which shows that cytotoxic T-lymphocyte fractions predominate in cancer tissues. 4,5,[13][14][15] The cytolytic potential of TILs usually is not expressed when tissue is freshly isolated, and, not infrequently, disease spread occurs despite the apparent presence of TILs. 16 -19 The changes of regional immune responses in the tumor milieu, especially the compositional relevance of TILs, may have important implications in terms of the progression of human malignancies.…”

Vaccination anti-HPV pour la prévention du cancer du col de l’utérus

“…Cependant, dans la mesure où les infections à HPV à haut risque sont au moins 2 fois plus fréquentes au niveau cervical que les infections à HPV à bas risque, et où seulement 5 femmes infectées sur mille verront leurs lésions progresser jusqu'à un grade élevé, l'immunité cellulaire anti-HPV16 semble être efficace chez la très grande majorité des femmes infectées. Réponses in situ Des lymphocytes T ont été mis en évidence in situ par des immunomarquages pratiqués sur des biopsies de condylomes régressifs [76] de VIN [77] de CIN [33,78] et de cancers cervicaux invasifs [79,80]. Il s'agissait de lymphocytes T CD4+ et CD8+ avec une prédominance de lymphocytes T CD4+ dans l'épithélium des condylomes, de lymphocytes T CD8+ dans l'épithélium des CIN ainsi que dans la tumeur et le chorion des cancers invasifs.…”

Human papillomavirus 16-specific T cell responses in classic HPV-related vulvar intra-epithelial neoplasia. Determination of strongly immunogenic regions from E6 and E7 proteins

“…Proliferative CD4 + T cell responses are also associated with spontaneously regressive CIN3 [17]. The evolution of CIN3 towards invasive cancers is featured by a decrease of CD4 + cellular infiltrate, an increase of CD8 + T lymphocytes [18][19][20], the appearance of suppressive T lymphocytes [21] and a loss of blood anti-HPV-16 CD4 + activity [22,23]. In high-grade CIN, positive intradermal reaction after intradermal injection of five HPV-16 E7 large peptides correlated with the spontaneous clearance of the lesions, which further indicates the presence and the very important role of HPV-specific CD4 + T lymphocytes [24].…”