Lymphotoxin cDNA Clones from a HTLV-I-Carrying T Cell Line HUT-102

Kato, Souichiro; Miki, Tetsuzou; Takahashi, Noriko; Ohmori, Muneki; Hemmi, Hiromichi; Numao, Naganori; Kondô, Kiyosi

doi:10.1089/aid.1989.5.663

Cited by 10 publications

(4 citation statements)

References 23 publications

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“…2), as a previously reported polymorphism in the 3' untranslated region was described with a frequency of 6% (25) . No homozygous 2.5-kb EcoRI sub- Figure (10,34,36) or contain in exchange ACC = Thr at amino acid position 26 (9, 35,36). We wondered whether the NcoI RFLP is linked with the presence of AAC at amino acid position 26.…”

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confidence: 99%

Polymorphic structure of the tumor necrosis factor (TNF) locus: an NcoI polymorphism in the first intron of the human TNF-beta gene correlates with a variant amino acid in position 26 and a reduced level of TNF-beta production.

Messer

Spengler

Jung

et al. 1991

The Journal of Experimental Medicine

527

360

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SummarySince a dysregulated synthesis of tumor necrosis factor a (TNF-a) may be involved in the pathogenesis of autoimmune diseases, it was ofinterest to precisely locate the recently reported Ncol restriction fragment length polymorphism (RFLP) of the TNF-a region . However, by mapping of 56.8 kb of overlapping cosmid clones and direct sequencing, we could localize the polymorphic Ncol restriction site within the first intron of the TNF-0 gene and not in the TNF-a gene. To study whether regulatory mechanisms are affected by this polymorphism, we analyzed the TNF-a/TNF-0 production of phytohemagglutinin-stimuated peripheral blood mononuclear cells ofindividuals homozygous for the TNF-0 Ncol RFLP by ELISA and concomitant Northern blot analysis. On days 2-4 after stimulation with mitogen, the TNFB*1 allele corresponding to a 5.3-kb Ncol fragment presented with a significantly higher TNF-0 response. A mRNA analysis demonstrated that higher protein levels of TNF-0 correlate also with increased amounts ofTNF-0 transcripts . No allelic association was found in respect to TNF-a production . To further investigate a possible allelic influence on transcription, we determined the DNA sequence of 2 kb ofthe 5' portion of our cloned TNFB*2 allele and compared it with the available TNF-0 sequences. By computer-aided recognition motif search ofDNA binding factors, we report putative binding sites conserved between mouse and man in the 5' flanking region as well as in intron 1 ofthe TNF-0 gene, found also in other cytokine promoter sequences. In addition, by polymerase chain reaction amplification and sequencing of 740 by of the 5' part of TNF-0 of individuals typed homozygously for the Ncol RFLP, we could show that amino acid position 26 is conserved as asparagine in the TNFB*1 and as threonine in the TNFB* 2 sequence. A previously reported, EcoRI RFLP in the 3' untranslated region of TNF-0 does not segregate with either of the two alleles. Thus, four TNFB alleles can de defined at the DNA level .

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confidence: 99%

Polymorphic structure of the tumor necrosis factor (TNF) locus: an NcoI polymorphism in the first intron of the human TNF-beta gene correlates with a variant amino acid in position 26 and a reduced level of TNF-beta production.

Messer

Spengler

Jung

et al. 1991

The Journal of Experimental Medicine

527

360

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“…In general, sighted persons receive about 80% of their external information through vision (1]. In Japan, the number of the visually impaired is estimated to be approximately 360,000, of which about a half are totally blind [2]. The visually impaired must use other senses, such as hearing and touch, to replace the lost visual information.…”

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confidence: 99%

“…It is reported, however, that 67% of the visually impaired are elderly people over 60 years of age [2]. It is also reported that the elderly exhibit the following tendencies [3].…”

Section: Introductionmentioning

confidence: 99%

A communication aid for deaf‐blind people using vibration motors

Kanno

Nagano

Asihara

et al. 1999

Electron. Comm. Jpn. Pt. III

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This paper proposes a Braille type input/output device as a communication aid for the deaf-blind. By means of this device, both input and output operations can be handled in 6-point braille format. The input/output unit of this device has a structure in which a vibration motor for the pager and a push-button switch are combined. Braille information can be input or presented using the same hand position. In presenting the Braille information, the palm sides of the forefinger, the middle finger, and the ring finger near the proximal and distal finger joints are used, to allow for ease during the input operation. In order to determine the optimal position for presenting Braille information in this device, an experiment was performed on single-point vibration stimulation sensitivity as well as two-point simultaneous vibration stimulation sensitivity. The experiment showed that the visually impaired subjects were able to recognize Braille information with a recognition rate of approximately 84%, after training for ten minutes. The input of Braille information was performed by a sighted subject, who could input the information at a rate of ap-proximately 36 character/min. after training for five hours. © 1998 Scripta Technica, Electron Comm Jpn Pt 3, 82(1): 918, 1999

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“…LT has recently been reported to be involved in the pathogenesis of human demyelinating disease, multiple sclerosis (8). In addition, the anti-tumor effects of LT are also reported (3). Therefore, the attempt to introduce LT gene into the CNS of an experimental host will give great potential to contributing to further research work on the CNS diseases.…”

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confidence: 99%

Expression of Lymphotoxin Gene Inserted into Theiler's Murine Encephalomyelitis Virus

Obuchi

Odagiri

Lizuka

et al. 1999

Microbiology and Immunology

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Theiler's murine encephalomyelitis virus (TMEV) belongs to the Picornaviridae genus. DA subgroup strains of this virus induce early, non-fatal polioencephalomyelitis followed by demyelination in the spinal cord, with virus persistence. We investigated the use of DA strain as avector for the introduction of a foreign gene into the central nervous system. Human lymphotoxin (LT) gene was inserted in the L region, the most upstream part of the polyprotein coding region of DA genome. Expression of LT was demonstrated by an immunoblot and an enzyme-linked immunosorbent assay on BHK-21 cells that were infected with the recombinant virus. In addition, the expressed LT showed cytotoxicity against L-929 cells.

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Lymphotoxin cDNA Clones from a HTLV-I-Carrying T Cell Line HUT-102

Cited by 10 publications

References 23 publications

Polymorphic structure of the tumor necrosis factor (TNF) locus: an NcoI polymorphism in the first intron of the human TNF-beta gene correlates with a variant amino acid in position 26 and a reduced level of TNF-beta production.

Polymorphic structure of the tumor necrosis factor (TNF) locus: an NcoI polymorphism in the first intron of the human TNF-beta gene correlates with a variant amino acid in position 26 and a reduced level of TNF-beta production.

A communication aid for deaf‐blind people using vibration motors

Expression of Lymphotoxin Gene Inserted into Theiler's Murine Encephalomyelitis Virus

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